使用膜过滤从墨西哥锡那罗亚culiacan当地超市的生鸡肉样品中回收弯曲杆菌

IF 0.5 Q4 BIOLOGY Revista Bio Ciencias Pub Date : 2019-10-11 DOI:10.15741/revbio.07.e698
J. Soto-Beltrán, B. Quiñones, A. Ibarra-Rodríguez, B. A. Amézquita-López
{"title":"使用膜过滤从墨西哥锡那罗亚culiacan当地超市的生鸡肉样品中回收弯曲杆菌","authors":"J. Soto-Beltrán, B. Quiñones, A. Ibarra-Rodríguez, B. A. Amézquita-López","doi":"10.15741/revbio.07.e698","DOIUrl":null,"url":null,"abstract":"Campylobacter  jejuni  and  Campylobacter coli  are responsible for human gastroenteritis worldwide, and the consumption of raw and undercooked poultry contributes to human infections. The present study examined the presence of  C. jejuni  and  C. coli  from retail chicken carcasses, purchased from local markets in the Culiacan Municipality in Sinaloa, Mexico.  To improve the recovery of  Campylobacter , the package liquid from the raw chicken carcasses was subjected to membrane filtration after an overnight enrichment under microaerophilic conditions.  The presence of  C. jejuni  and  C. coli  was further determined by PCR amplification of the  lpxA  gene.  Presumptive  Campylobacter  colonies were recovered from 56.7% (17/30) of the chicken carcasses purchased from 63.6% (7/11) of the retail markets examined.  Genotyping assays detected  C . jejuni  in 33.3% (10/30) of the carcasses examined.  This study identified for the first time  C. jejuni  in raw chicken carcasses in Northwestern Mexico and provided valuable information for monitoring  Campylobacter in retail meats.","PeriodicalId":41763,"journal":{"name":"Revista Bio Ciencias","volume":" ","pages":""},"PeriodicalIF":0.5000,"publicationDate":"2019-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Uso de filtración por membrana para la recuperación de Campylobacter de muestras de pollo crudo provenientes de supermercados locales en Culiacán, Sinaloa, México\",\"authors\":\"J. Soto-Beltrán, B. Quiñones, A. Ibarra-Rodríguez, B. A. Amézquita-López\",\"doi\":\"10.15741/revbio.07.e698\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Campylobacter  jejuni  and  Campylobacter coli  are responsible for human gastroenteritis worldwide, and the consumption of raw and undercooked poultry contributes to human infections. The present study examined the presence of  C. jejuni  and  C. coli  from retail chicken carcasses, purchased from local markets in the Culiacan Municipality in Sinaloa, Mexico.  To improve the recovery of  Campylobacter , the package liquid from the raw chicken carcasses was subjected to membrane filtration after an overnight enrichment under microaerophilic conditions.  The presence of  C. jejuni  and  C. coli  was further determined by PCR amplification of the  lpxA  gene.  Presumptive  Campylobacter  colonies were recovered from 56.7% (17/30) of the chicken carcasses purchased from 63.6% (7/11) of the retail markets examined.  Genotyping assays detected  C . jejuni  in 33.3% (10/30) of the carcasses examined.  This study identified for the first time  C. jejuni  in raw chicken carcasses in Northwestern Mexico and provided valuable information for monitoring  Campylobacter in retail meats.\",\"PeriodicalId\":41763,\"journal\":{\"name\":\"Revista Bio Ciencias\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.5000,\"publicationDate\":\"2019-10-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista Bio Ciencias\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15741/revbio.07.e698\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Bio Ciencias","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15741/revbio.07.e698","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

空肠弯曲杆菌和大肠弯曲杆菌是全世界人类肠胃炎的主因,食用生的和未煮熟的家禽会导致人类感染。本研究检查了从墨西哥锡那罗亚州库利亚坎市当地市场购买的零售鸡尸体中是否存在空肠杆菌和大肠杆菌。为了提高弯曲杆菌的回收率,将生鸡胴体的包装液在微气条件下富集过夜后进行膜过滤。通过lpxA基因的PCR扩增进一步确定了空肠杆菌和大肠杆菌的存在。从63.6%(7/11)的零售市场购买的鸡尸体中检出56.7%(17/30)的推测弯曲杆菌菌落。基因分型检测到C。33.3%(10/30)为空肠菌。本研究首次在墨西哥西北部的生鸡肉中发现了空肠梭菌,为零售肉类中弯曲杆菌的监测提供了有价值的信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Uso de filtración por membrana para la recuperación de Campylobacter de muestras de pollo crudo provenientes de supermercados locales en Culiacán, Sinaloa, México
Campylobacter  jejuni  and  Campylobacter coli  are responsible for human gastroenteritis worldwide, and the consumption of raw and undercooked poultry contributes to human infections. The present study examined the presence of  C. jejuni  and  C. coli  from retail chicken carcasses, purchased from local markets in the Culiacan Municipality in Sinaloa, Mexico.  To improve the recovery of  Campylobacter , the package liquid from the raw chicken carcasses was subjected to membrane filtration after an overnight enrichment under microaerophilic conditions.  The presence of  C. jejuni  and  C. coli  was further determined by PCR amplification of the  lpxA  gene.  Presumptive  Campylobacter  colonies were recovered from 56.7% (17/30) of the chicken carcasses purchased from 63.6% (7/11) of the retail markets examined.  Genotyping assays detected  C . jejuni  in 33.3% (10/30) of the carcasses examined.  This study identified for the first time  C. jejuni  in raw chicken carcasses in Northwestern Mexico and provided valuable information for monitoring  Campylobacter in retail meats.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
20
审稿时长
32 weeks
期刊最新文献
Análisis del ejercicio de las instituciones, extorsiones y vínculos exteriores sobre la estructura productiva de una economía rural Condición reproductiva de Centropomus robalito (Perciformes: Centropomidae) en la laguna costera de Chautengo, Guerrero, México Registros norteños de Mazama temama y de Leopardus wiedii en el noreste de México Microbiological quality assessment of treated municipal wastewater effluents for crop irrigation purposes Optimización de la hidrolisis enzimática del almidón en el jugo de caña de azúcar (Saccharum officinarum)
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1