制备用于毒理学反应病理和生化分析的特定肺室

Laura S. Van Winkle, Jacklyn S. Kelty, Charles G. Plopper
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引用次数: 5

摘要

本单元侧重于评估胸肺组织中微环境特异性反应的方案。整个呼吸系统的各个方面都是候选毒物的潜在靶点,但由于毒物或靶细胞的不同分布,每种候选毒物可能影响不同的部位。在传导气道内,驻留细胞群的组成和细胞群的代谢能力变化很大。因此,对肺这一区域的研究需要独特的、部位选择性的方法来明确定义毒性反应。如本章所述,如果没有特定地点的采样,在传导气道中检测毒性效应的实验极限就会被削弱,因为与治疗无关但与位置有关的差异可能会主导反应。这里包括的方案允许评估气管支气管和肺气体交换区域的毒理学反应,并具体应用于实验室哺乳动物。©2017 by John Wiley &儿子,Inc。
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Preparation of Specific Compartments of the Lungs for Pathologic and Biochemical Analysis of Toxicologic Responses

This unit focuses on protocols for assessing microenvironment-specific responses in the thoracic lung tissues. Aspects of the entire respiratory system serve as potential targets for candidate toxicants, but each candidate toxicant may impact distinct sites due to differential distribution of either the toxicant or the target cells. Within the conducting airways, the composition of resident cell populations and the metabolic capabilities of the cell populations vary greatly. Thus, studies of this region of the lung require unique, site-selective methods to clearly define the toxic response. Without site-specific sampling, as described in this chapter, the experimental limit of detection for toxicant effects in conducting airways is weakened because differences unrelated to treatment, but related to location, may dominate the response. The protocols included here allow assessment of toxicological responses in the tracheobronchial airways and the gas exchange area of the lung, with specific application to laboratory mammals. © 2017 by John Wiley & Sons, Inc.

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