Zn2+诱导霍山石斛线粒体呼吸链复合物活性和花挥发物排放的变化

IF 2.2 4区 农林科学 Q2 HORTICULTURE Folia Horticulturae Pub Date : 2022-06-01 DOI:10.2478/fhort-2022-0009
Wangsheng Zhu, Jun Dai, Jiahong Wang
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引用次数: 1

摘要

摘要近年来,植物挥发物因其在繁殖、自我保护等方面的重要功能而受到越来越多的关注。虽然在各种环境因素下对花挥发物排放的解析和相关机制方面取得了一些进展,但Zn2+浓度对线粒体功能和花挥发物排放的影响尚未揭示。以霍山石斛花瓣为材料,用0 ~ 8 mM的ZnSO4溶液处理。采用荧光染料法和原子吸收法测定细胞内Zn2+浓度。采用生化法测定线粒体呼吸链复合体活性、前体及ATP含量。采用气相色谱-质谱联用仪(GC-MS)分析了花的挥发性成分。结果表明,施用ZnSO4显著提高了细胞内Zn2+浓度。细胞内Zn2+浓度升高对线粒体呼吸链复合体活性、前体和三磷酸腺苷(ATP)的产生以及花挥发物排放有不同程度的影响。此外,线粒体呼吸链复合物的活性、前体和ATP的产生与花挥发物的排放呈正相关。由此可见,Zn2+浓度可诱导线粒体呼吸链复合物,尤其是复合物II和V的活性变化,通过影响与萜类、苯甲酸和脂肪酸衍生物产生密切相关的前体和ATP水平,促进或抑制花挥发物的排放。该研究将有助于从线粒体功能的角度了解Zn2+浓度与花挥发物排放的关系。
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Zn2+ induces changes in activities of mitochondrial respiratory chain complexes and emissions of floral volatiles in Dendrobium huoshanense
Abstract In recent years, there has been increasing interest in floral volatiles due to their important function in reproduction, self-protection and so on. Although some progress was made on deciphering emissions of floral volatiles and on related mechanisms under a variety of environmental factors, the effects of Zn2+ concentrations on mitochondrial function and floral volatile emissions are yet to be revealed. Dendrobium huoshanense petals were used as materials and were treated with a 0–8 mM ZnSO4 solution. Intracellular Zn2+ concentrations were evaluated by the fluorescent dye method and atomic absorption method. Mitochondrial respiratory chain complex activities and the precursor and ATP contents were determined by the biochemical method. Floral volatile components were analyzed by the gas chromatograph-mass spectrometer (GC-MS). The results indicated that ZnSO4 application significantly increased intracellular Zn2+ concentrations. Elevated intracellular Zn2+ concentrations differently affected mitochondrial respiratory chain complex activities, precursor and adenosine triphosphate (ATP) productions and floral volatile emissions. Moreover, positive correlations exist among the activities of mitochondrial respiratory chain complexes, productions of precursors and ATP and emissions of floral volatiles. It is concluded that Zn2+ concentrations induce the activity changes of mitochondrial respiratory chain complexes, especially complex II and V, which promote or inhibit the emissions of floral volatiles by affecting the precursor and ATP levels that are closely related to the production of terpenoids, benzoids and fatty acid derivatives. The research will contribute to understanding the relationship between Zn2+ concentrations and floral volatile emissions from the perspective of mitochondrial function.
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来源期刊
Folia Horticulturae
Folia Horticulturae Agricultural and Biological Sciences-Horticulture
CiteScore
3.40
自引率
0.00%
发文量
13
审稿时长
16 weeks
期刊介绍: Folia Horticulturae is an international, scientific journal published in English. It covers a broad research spectrum of aspects related to horticultural science that are of interest to a wide scientific community and have an impact on progress in both basic and applied research carried out with the use of horticultural crops and their products. The journal’s aim is to disseminate recent findings and serve as a forum for presenting views as well as for discussing important problems and prospects of modern horticulture, particularly in relation to sustainable production of high yield and quality of horticultural products, including their impact on human health.
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