微生物对生物合成有机物内生输入的土壤呼吸反应及其在固碳中的意义

IF 0.9 4区 环境科学与生态学 Q4 ENVIRONMENTAL SCIENCES Carpathian Journal of Earth and Environmental Sciences Pub Date : 2023-01-25 DOI:10.26471/cjees/2023/018/240
S. Matei, G. Matei, S. Dumitru, V. Mocanu
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引用次数: 0

摘要

活性C作为有机物化学氧化水平的衡量标准,反映了微生物可获得的碳。土壤呼吸作为估计土壤微生物活性的直接方法,可以测量土壤中碳储量的波动。为了确定土壤呼吸评估这种波动的能力,我们使用了有机碳含量增加的土壤,在恒定的最佳条件下,消除干扰因素,并在短时间内进行了分析。在标准实验条件下,通过对土壤呼吸的现场测定和对非原位土壤样品中CO2通量的分析来评估这些土壤的比分解速率的影响,以突出这些土壤中的碳储存。可以通过分析这些参数来积累参考数据,这些参数与关于微生物生物量含量、黄腐菌亚组分含量、溶解有机物质的荧光和铁载体含量演变的定量/定性测定结果相比,可以通过它们自身的演变来考虑,作为支持在有效估计土壤中碳储存演变中使用呼吸的论据。这些参数的分析分两个阶段进行,用于比较实验的初始和最终数据(30天后)。不同阶段土壤的呼吸潜能水平不同。随着时间的推移,每种土壤的呼吸水平降低了4.27-14.60%。随着时间的推移,两种土壤的CO2通量都呈现出持续下降的趋势。在Mollic Histic Gleysol(Salinic)的情况下,对于在最终阶段确定的通量,确定系数的值为R2=0.92。实验结束时,两种土壤的微生物生物量水平都显著增加。在Mollic Histic Gleysol(Salinac)的情况下,微生物生物量从456±23.12μgC∙g-1增加到514±24.57μgC・g-1土壤。在标准条件下培养30天后,两种土壤的黄腐病亚组分A-D都显示出具有不同分子量和复杂程度的可溶性有机化合物的显著积累。利用成像法对水可提取有机物中的荧光成分进行了重点分析。新生物合成的碳化合物在软体动物组织Gleysol(Salinic)的有机物中的储存程度最高。铁载体生物合成的强度随着时间的推移而增加,从最初在Mollic Gleysol(Salinic)中较低的存在开始(具有Ö11 mm光晕),随后在实验期结束时铁载体含量和铁的可用性增加。Mollic Histic Gleysol(Salinic)中铁载体的积累确定了直径为PXS 31 mm的晕圈。
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SOIL RESPIRATION AS MICROBIAL RESPONSE TO THE ENDOGEN INPUT OF BIO-SYNTHESIZED ORGANIC MATTER AND ITS IMPLICATION IN CARBON SEQUESTRATION
Active C, as a measure of the level of chemical oxidation of organic matter, reflects the carbon available to microorganisms. Soil respiration, as a direct way to estimate edaphic microbial activity, could be a measure of the fluctuations of carbon stocks in soils. To determine the ability of soil respiration to evaluate such fluctuations we used soils with an increased content of organic carbon, constant optimal conditions, to eliminate the disturbing factors, analyzed in a short period of time. The influence of the specific decomposition rates of these soils was assessed by both spot determinations of soil respiration and analyzing the flux of CO2 from ex situ soil samples, under standard experimental conditions, to highlight carbon storage in such soils. Reference data can be accumulated through the analysis of these parameters, which compared with the results of quantitative/qualitative determinations regarding the changes in the content of microbial biomass, the content of fulvic sub-fractions, the fluorescence of dissolved organic material and the evolution of the content of siderophores, could be considered, by their own evolutions, as arguments in sustaining the use of respiration in the efficient estimation of carbon storage evolution in the soils. The analyses of these parameters were carried out in two phases, for comparing initial and final data of experiment (after 30 days). The soils had different levels of the respiration potential between phases. The level of soil respiration was reduced in time between 4.27-14.60%, in each soil. The CO2 flux showed, in time, a continuous decreasing trend in both soils. In the case of Mollic Histic Gleysol (Salinic), the coefficient of determination has the value R2=0.92 for the flux determined in the final phase. The levels of microbial biomass of both soils were increased significantly at the end of the experiment. In the case of Mollic Histic Gleysol (Salinic), microbial biomass increased from 456±23.12 μgC∙g-1 to 514±24.57 μgC∙g-1 soil. The fulvic sub-fractions A-D of both soils revealed significant accumulates of soluble organic compounds, with different molecular weights and complexity levels, after 30 days of incubations in standard conditions. The fluorescent components present in the water-extractable organic matter were highlighted by imagistic method. The highest degree of storages of newly bio-synthesized compounds of carbon was registered in organic matter of Mollic Histic Gleysol (Salinic). The intensity of siderophores biosynthesis increased over time, starting from an initial lower presence in the Mollic Gleysol (Salinic) (with Ø 11 mm halo), which were followed by an increasing of siderophores content and availability of iron, at the end of the experimental period. Accumulations of siderophores in the Mollic Histic Gleysol (Salinic) determined a Ø 31 mm halo diameter.
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来源期刊
CiteScore
2.30
自引率
25.00%
发文量
42
审稿时长
12-24 weeks
期刊介绍: The publishing of CARPATHIAN JOURNAL of EARTH and ENVIRONMENTAL SCIENCES has started in 2006. The regularity of this magazine is biannual. The magazine will publish scientific works, in international purposes, in different areas of research, such as : geology, geography, environmental sciences, the environmental pollution and protection, environmental chemistry and physic, environmental biodegradation, climatic exchanges, fighting against natural disasters, protected areas, soil degradation, water quality, water supplies, sustainable development.
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