{"title":"远红光和营养水平对夏枯草离体培养物生长和次生代谢产物的影响","authors":"Caiyun Chen, Jing-Ping Yang, Wen-Dar Huang, Chang-Chang Chen","doi":"10.3390/agronomy13092250","DOIUrl":null,"url":null,"abstract":"Prunella vulgaris, a medicinal plant with antioxidant capacity, was investigated for its response to varying intensities of far-red light and nutrient levels. Plantlets were cultured for 30 d under low far-red light (LFR) or high far-red light (HFR) conditions and different nutrient levels (full, half, and quarter). HFR reduced leaf and branch number, dry weight, and accumulation of chlorophylls (Chl) and carotenoids (Car), while increasing plant height. Lower nutrient levels increased plant height and leaf number, but decreased branch number, Chl, and Car. HFR significantly increased total phenolic content (TPC), rutin, and rosmarinic acid levels, while total flavonoid content decreased. As nutrient levels decreased, TPC and rosmarinic acid declined. HFR induced significant DPPH scavenging activity, while reducing power increased with higher far-red light and nutrient levels. The ferrous ion chelating effect under LFR reduced with lower nutrient levels. There were strong correlations among TPC, rosmarinic acid, DPPH scavenging activity, and reducing power. In conclusion, HFR inhibited plantlet growth but enhanced secondary metabolite accumulation and antioxidant capacity. Different nutrient levels stimulated diverse growth responses, while elevated nutrient levels promoted secondary metabolite production. This study demonstrated the responses of growth, secondary metabolite accumulation, and antioxidant activity in the in vitro cultured P. vulgaris to supplemental far-red light and various nutrient levels.","PeriodicalId":56066,"journal":{"name":"Agronomy-Basel","volume":" ","pages":""},"PeriodicalIF":3.3000,"publicationDate":"2023-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Effect of Far-Red Light and Nutrient Level on the Growth and Secondary Metabolites of the In Vitro Culture of Prunella vulgaris\",\"authors\":\"Caiyun Chen, Jing-Ping Yang, Wen-Dar Huang, Chang-Chang Chen\",\"doi\":\"10.3390/agronomy13092250\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Prunella vulgaris, a medicinal plant with antioxidant capacity, was investigated for its response to varying intensities of far-red light and nutrient levels. Plantlets were cultured for 30 d under low far-red light (LFR) or high far-red light (HFR) conditions and different nutrient levels (full, half, and quarter). HFR reduced leaf and branch number, dry weight, and accumulation of chlorophylls (Chl) and carotenoids (Car), while increasing plant height. Lower nutrient levels increased plant height and leaf number, but decreased branch number, Chl, and Car. HFR significantly increased total phenolic content (TPC), rutin, and rosmarinic acid levels, while total flavonoid content decreased. As nutrient levels decreased, TPC and rosmarinic acid declined. HFR induced significant DPPH scavenging activity, while reducing power increased with higher far-red light and nutrient levels. The ferrous ion chelating effect under LFR reduced with lower nutrient levels. There were strong correlations among TPC, rosmarinic acid, DPPH scavenging activity, and reducing power. In conclusion, HFR inhibited plantlet growth but enhanced secondary metabolite accumulation and antioxidant capacity. Different nutrient levels stimulated diverse growth responses, while elevated nutrient levels promoted secondary metabolite production. This study demonstrated the responses of growth, secondary metabolite accumulation, and antioxidant activity in the in vitro cultured P. vulgaris to supplemental far-red light and various nutrient levels.\",\"PeriodicalId\":56066,\"journal\":{\"name\":\"Agronomy-Basel\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2023-08-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Agronomy-Basel\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.3390/agronomy13092250\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agronomy-Basel","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3390/agronomy13092250","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
The Effect of Far-Red Light and Nutrient Level on the Growth and Secondary Metabolites of the In Vitro Culture of Prunella vulgaris
Prunella vulgaris, a medicinal plant with antioxidant capacity, was investigated for its response to varying intensities of far-red light and nutrient levels. Plantlets were cultured for 30 d under low far-red light (LFR) or high far-red light (HFR) conditions and different nutrient levels (full, half, and quarter). HFR reduced leaf and branch number, dry weight, and accumulation of chlorophylls (Chl) and carotenoids (Car), while increasing plant height. Lower nutrient levels increased plant height and leaf number, but decreased branch number, Chl, and Car. HFR significantly increased total phenolic content (TPC), rutin, and rosmarinic acid levels, while total flavonoid content decreased. As nutrient levels decreased, TPC and rosmarinic acid declined. HFR induced significant DPPH scavenging activity, while reducing power increased with higher far-red light and nutrient levels. The ferrous ion chelating effect under LFR reduced with lower nutrient levels. There were strong correlations among TPC, rosmarinic acid, DPPH scavenging activity, and reducing power. In conclusion, HFR inhibited plantlet growth but enhanced secondary metabolite accumulation and antioxidant capacity. Different nutrient levels stimulated diverse growth responses, while elevated nutrient levels promoted secondary metabolite production. This study demonstrated the responses of growth, secondary metabolite accumulation, and antioxidant activity in the in vitro cultured P. vulgaris to supplemental far-red light and various nutrient levels.
Agronomy-BaselAgricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
6.20
自引率
13.50%
发文量
2665
审稿时长
20.32 days
期刊介绍:
Agronomy (ISSN 2073-4395) is an international and cross-disciplinary scholarly journal on agronomy and agroecology. It publishes reviews, regular research papers, communications and short notes, and there is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.