纯橙色叶子作为熏蒸剂对储存中的可可种子的潜在危害

P. Atikah, I. Harahap, D. Sartiami
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引用次数: 0

摘要

可可(Theobroma cacao)是印度尼西亚的重要商品之一。可可豆的品质在储存过程中下降,其中一个原因是由于Araecerus fasciculatus (De Geer)的攻击。这种虫害可以通过使用精油对环境安全的熏蒸来克服。采用气相色谱-质谱联用技术,研究青柠叶精油(kaffir lime leaves精油,KLLEO)对束腹沙蝇(A. fasciculatus)的致死性和驱避作用,并分析其有效部位的化合物含量。研究阶段包括维持和昆虫饲养,毒性和驱避试验,分离和分析精油的活性部分化合物。采用CRD设计,6个处理,4个重复。在熏蒸72 h后,KLLEO正己烷组分对束束夜蛾成虫的死亡率最高。KLLEO正己烷部分的LD50和LD95值分别为0.30和1.66 ml/l空气。空气浓度为1 ml/l时,成虫死亡率最高,达73.75%。用嗅觉仪检测,KLLEO对成虫有较高的驱避力,在1 ml/l空气中驱避指数可达100%。KLLEO正己烷组分中最主要的化合物是香茅醛。因此,KLLEO具有一定的推广应用潜力,可作为植物熏蒸剂应用于束甲。
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Potensi minyak atsiri daun jeruk purut sebagai fumigan nabati terhadap Araecerus fasciculatus (De Geer) (Coleoptera: Anthribidae) pada biji kakao di tempat penyimpanan
Cocoa (Theobroma cacao) is one of the important commodities in Indonesia. The quality of cocoa beans has decreased during storage, one of which is due to the attack of Araecerus fasciculatus (De Geer). This pest attack can be overcome by fumigation that is safe for the environment through the use of essential oil. The study aimed to examine the mortality and repellent effects of kaffir lime leaves essential oil (KLLEO) to A. fasciculatus and to analyze the compound content of active fraction KLLEO using GC-MS. Research stages included maintenance and insect rearing, toxicity and repellency tests, fractionation, and analysis of active fraction compounds of essential oils. The study was designed using CRD with 6 treatments and 4 replications. The n-hexane fraction of KLLEO caused the highest mortality of A. fasciculatus adults after 72 hours of fumigation. The LD50 and LD95 values of the n-hexane fraction of KLLEO were 0.30 and 1.66 ml/l air, respectively. The dose at 1 ml/l air caused the highest mortality of A. fasciculatus adult by 73.75%. Examine using an olfactometer, KLLEO has high repellency against adult A. fasciculatus with the value of the repellency index reaching 100% at 1 ml/l air. The most dominant compound in the n-hexane fraction of KLLEO was the citronellal. Therefore, the KLLEO has some potential to be expanded as a botanical fumigant towards A. fasciculatus.
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