鱼藤酮处理海马神经元中l -丝氨酸对溶酶体的影响

Q3 Immunology and Microbiology Applied Microscopy Pub Date : 2023-01-10 DOI:10.1186/s42649-022-00084-z

Sumin Shin, Su-Kyeong Hwang, Ji Young Mun

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引用次数: 0

摘要

氧化应激会破坏细胞器，破坏DNA，最终导致大脑退行性疾病。氧化应激引起的持续线粒体损伤最终导致细胞抑制溶酶体的功能。本研究中使用的鱼藤酮抑制线粒体电子传递链复合体1。由于这种抑制，自由基的产生被促进，并可能发生氧化应激。为了测试l -丝氨酸作为抗氧化剂的作用，在鱼藤酮治疗前处理l -丝氨酸对HT22海马细胞的作用。然后分析溶酶体活性和结构的变化。结果表明，鱼藤酮引起的HT22细胞氧化应激受到l -丝氨酸的保护。l -丝氨酸减少了细胞中的自由基，并保护了受损的溶酶体结构和活性。

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Changes of lysosome by L-serine in rotenone-treated hippocampal neurons

Oxidative stress destroys cellular organelles and damages DNA, eventually leading to degenerative brain disorders. Persistent mitochondrial damage by oxidative stress eventually causes cells to inhibit the function of lysosomes. Rotenone used in this study inhibits complex 1 of the mitochondrial electron transport chain. Due to this inhibition, the production of free radicals is promoted, and oxidative stress can occur. To test as a role of antioxidant, L-serine was treated before treatment of rotenone to HT22 hippocampal cells. Then, changes in the activity and structure of lysosomes were analyzed. As a result, the oxidative stress caused by rotenone in HT22 cells was protected by L-serine. L-serine reduced free radicals in cells, and the damaged lysosomal structure and lysosome activity were also protected.

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来源期刊

Applied Microscopy Immunology and Microbiology-Applied Microbiology and Biotechnology

CiteScore

3.40

自引率

0.00%

发文量

审稿时长

10 weeks

期刊介绍： Applied Microscopy is a peer-reviewed journal sponsored by the Korean Society of Microscopy. The journal covers all the interdisciplinary fields of technological developments in new microscopy methods and instrumentation and their applications to biological or materials science for determining structure and chemistry. ISSN: 22875123, 22874445.