The Tolerability and Effectiveness of Marine-Based Ingredients in Cosmetics: A Split-Face Clinical Study of a Serum Spray Containing Fucus vesiculosus Extract, Ulva lactuca Extract, and Ectoin

Introduction: Marine-derived compounds, such as seaweed extracts, fucoidan and ulvans, and ectoin, have gained attention in recent years due to their unique structural and functional characteristics, which make them attractive ingredients for skincare products. In this study, we developed a serum spray based on fucoidan, Ulva lactuca extract, and ectoin and evaluated its efficacy on facial skin. Materials and Methods: A split-face design dermatological evaluation of the serum spray was conducted on 33 subjects with visible signs of skin aging, with 29 subjects completing the study according to its protocol. The subjects had a mean age of 50 years and 16 had sensitive skin. The instrumental efficacy and subjective efficacy of the spray were measured on facial skin by evaluating the trans-epidermal water loss (TEWL), skin pH, skin roughness/wrinkle reduction, and skin hydration at baseline, 20 min after its application and 28 days after its continuous use. Results: We found that the application of the serum spray did not significantly affect the TEWL. The hydration in the area treated with Moisturizer + Spray was 17% higher than that in the area treated with Moisturizer alone after 20 min of application (p < 0.001) and 5% higher after 28 days of use (p < 0.05). Twenty minutes after the application of the product, the average roughness in the area treated with Moisturizer + Spray decreased significantly, with an average of 7% compared to baseline (p < 0.001). With regard to the long-term antiwrinkle effect, 28 days after the continuous use of the product, the average roughness in the area treated with Moisturizer + Spray decreased significantly, with an average of 17% in relation to baseline (p < 0.001). The skin pH was significantly lowered by 6% after 28 days of use of the moisturizer + spray (p < 0.05). Conclusion: The results of this study suggest that the marine-derived compounds fucoidan, ulvans, and ectoin have hydrating and anti-wrinkle properties that make them effective ingredients for skincare products. The serum spray developed in this study was demonstrated to be safe and increase hydration, showing a reduction in wrinkles and maintenance of the skin barrier function after 28 days of its continuous use. Therefore, it could be a promising addition to skincare products for improving skin health.