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引用次数: 15
摘要
摘要/ ABSTRACT摘要:对棘翅目:棘翅科(Acanthacorydalis fruhstorferi van der Weele)蝇类进行了线粒体全基因组测序。该基因组全长15286 bp,包括13个蛋白质编码基因、22个转移RNA基因(tRNAs)、2个核糖体RNA基因(rnas)和一个假定的a + t富区。基因排列与大多数常见的大翅目线粒体基因组相同。我们分析了蛋白质编码基因的首选密码子使用,并预测了所有RNA转录本的二级结构。此外,基于13个蛋白编码基因的序列构建了系统发育树,结果支持了目前大翅目与神经翅目亲缘关系密切的观点。我们的分析表明,完整的线粒体基因组序列是一种很有希望的方法来全面解决大翅目动物的系统发育关系。
Characterization of the Complete Mitochondrial Genome of Acanthacorydalis fruhstorferi van der Weele (Megaloptera: Corydalidae)
ABSTRACT: We sequenced the complete mitochondrial genome of the fishfly species Acanthacorydalis fruhstorferi van der Weele (Megaloptera: Corydalidae). The genome, which was 15,286 bp in length, included a standard set of 13 protein-coding genes, 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), and a putative A+T-rich region. The gene arrangement is identical to that of most common Megaloptera mitochondrial genomes. We analyzed the preferred codon usage of the protein-coding genes and predicted the secondary structures of all the RNA transcripts. In addition, a phylogenetic tree was constructed based on the sequences of the 13 protein-coding genes, and the results supported the current view of a close relationship between Megaloptera and Neuroptera. Our analyses suggest that complete mitochondrial genome sequences are a promising method to fully resolve the phylogenetic relationships within Megaloptera.
期刊介绍:
The Journal of the Kansas Entomological Society is a publication of the Kansas (Central States) Entomological Society, publishes research on all aspects of the sciences of entomology, and has world-wide authorship and readership.