四川硬皮病气味结合蛋白的分子特征、表达及结合特异性分析(膜翅目:白茧蜂科)

IF 0.2 4区 农林科学 Q4 ENTOMOLOGY Journal of the Kansas Entomological Society Pub Date : 2020-01-08 DOI:10.2317/0022-8567-92.2.459
Yuan-Yuan Tao, Xiao-qing Zhu, Wei Yang, Hua Yang, Chun-ping Yang, Fengrong Guan, Qing-Long Han
{"title":"四川硬皮病气味结合蛋白的分子特征、表达及结合特异性分析(膜翅目:白茧蜂科)","authors":"Yuan-Yuan Tao, Xiao-qing Zhu, Wei Yang, Hua Yang, Chun-ping Yang, Fengrong Guan, Qing-Long Han","doi":"10.2317/0022-8567-92.2.459","DOIUrl":null,"url":null,"abstract":"Abstract: Odorant-binding proteins (OBPs) play an important role in the sense of smell in parasitoid wasps, especially for locating hosts and mates. Clarifying the role of OBPs in information exchanges between parasitoid wasps and their environment is beneficial for understanding the sense of smell in parasitoid wasps. To this end, we cloned the OBPs, SsicOBP1 and SsicOBP2 (GenBank accession numbers: AKP80533.1 and AKP80534.1), of Scleroderma sichuanensis Xiao and then proceeded to conduct prokaryotic expression and qPCR analysis, and determine the binding capacity of SsicOBP1 and SsicOBP2 with 18 types of volatiles from Pinus massoniana Lambert using competitive binding (with 1-NPN as fluorescent probes). We found that SsicOBP1 and SsicOBP2 are expressed at high levels in the antennae of Scleroderma sichuanensis Xiao. Of the 18 types of P. massoniana volatiles, SsicOBP1 could bind effectively with ocimene (binding constant of 12.82 µM) and β-pinene (binding constant of 15.57 µM). SsicOBP2 could bind effectively with α-pinene (with a binding constant of 13.52 µM), ocimene (binding constant of 14.38 µM) and β-pinene (binding constant of 14.51 µM). From this, we could infer that SsicOBP1 and SsicOBP2 are general odorant-binding proteins, which play an important role in sensing plant volatiles.","PeriodicalId":17396,"journal":{"name":"Journal of the Kansas Entomological Society","volume":null,"pages":null},"PeriodicalIF":0.2000,"publicationDate":"2020-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Molecular Characterization, Expression and Binding Specificity Analysis of the Odorant-Binding Proteins of Scleroderma sichuanensis Xiao (Hymenoptera: Bethylidae)\",\"authors\":\"Yuan-Yuan Tao, Xiao-qing Zhu, Wei Yang, Hua Yang, Chun-ping Yang, Fengrong Guan, Qing-Long Han\",\"doi\":\"10.2317/0022-8567-92.2.459\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract: Odorant-binding proteins (OBPs) play an important role in the sense of smell in parasitoid wasps, especially for locating hosts and mates. Clarifying the role of OBPs in information exchanges between parasitoid wasps and their environment is beneficial for understanding the sense of smell in parasitoid wasps. To this end, we cloned the OBPs, SsicOBP1 and SsicOBP2 (GenBank accession numbers: AKP80533.1 and AKP80534.1), of Scleroderma sichuanensis Xiao and then proceeded to conduct prokaryotic expression and qPCR analysis, and determine the binding capacity of SsicOBP1 and SsicOBP2 with 18 types of volatiles from Pinus massoniana Lambert using competitive binding (with 1-NPN as fluorescent probes). We found that SsicOBP1 and SsicOBP2 are expressed at high levels in the antennae of Scleroderma sichuanensis Xiao. Of the 18 types of P. massoniana volatiles, SsicOBP1 could bind effectively with ocimene (binding constant of 12.82 µM) and β-pinene (binding constant of 15.57 µM). SsicOBP2 could bind effectively with α-pinene (with a binding constant of 13.52 µM), ocimene (binding constant of 14.38 µM) and β-pinene (binding constant of 14.51 µM). From this, we could infer that SsicOBP1 and SsicOBP2 are general odorant-binding proteins, which play an important role in sensing plant volatiles.\",\"PeriodicalId\":17396,\"journal\":{\"name\":\"Journal of the Kansas Entomological Society\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.2000,\"publicationDate\":\"2020-01-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the Kansas Entomological Society\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.2317/0022-8567-92.2.459\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Kansas Entomological Society","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2317/0022-8567-92.2.459","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 2

摘要

摘要/ Abstract摘要:气味结合蛋白(OBPs)在寄生蜂的嗅觉功能中起着重要的作用,特别是在定位寄主和配偶方面。阐明OBPs在寄生蜂与环境信息交换中的作用,有助于理解寄生蜂的嗅觉。为此,我们克隆了四川硬皮病萧的OBPs, SsicOBP1和SsicOBP2 (GenBank登录号:AKP80533.1和AKP80534.1),进行原核表达和qPCR分析,并采用竞争结合(以1-NPN为荧光探针)测定了SsicOBP1和SsicOBP2与马尾松18种挥发物的结合能力。我们发现SsicOBP1和SsicOBP2在四川硬皮病小虫的触角中高水平表达。在18种马尾草挥发物中,SsicOBP1能与辛烯(结合常数为12.82µM)和β-蒎烯(结合常数为15.57µM)有效结合。sicobp2能与α-蒎烯(结合常数为13.52µM)、辛烯(结合常数为14.38µM)和β-蒎烯(结合常数为14.51µM)有效结合。由此我们可以推断,SsicOBP1和SsicOBP2是一般的气味结合蛋白,在植物挥发物的感知中发挥重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Molecular Characterization, Expression and Binding Specificity Analysis of the Odorant-Binding Proteins of Scleroderma sichuanensis Xiao (Hymenoptera: Bethylidae)
Abstract: Odorant-binding proteins (OBPs) play an important role in the sense of smell in parasitoid wasps, especially for locating hosts and mates. Clarifying the role of OBPs in information exchanges between parasitoid wasps and their environment is beneficial for understanding the sense of smell in parasitoid wasps. To this end, we cloned the OBPs, SsicOBP1 and SsicOBP2 (GenBank accession numbers: AKP80533.1 and AKP80534.1), of Scleroderma sichuanensis Xiao and then proceeded to conduct prokaryotic expression and qPCR analysis, and determine the binding capacity of SsicOBP1 and SsicOBP2 with 18 types of volatiles from Pinus massoniana Lambert using competitive binding (with 1-NPN as fluorescent probes). We found that SsicOBP1 and SsicOBP2 are expressed at high levels in the antennae of Scleroderma sichuanensis Xiao. Of the 18 types of P. massoniana volatiles, SsicOBP1 could bind effectively with ocimene (binding constant of 12.82 µM) and β-pinene (binding constant of 15.57 µM). SsicOBP2 could bind effectively with α-pinene (with a binding constant of 13.52 µM), ocimene (binding constant of 14.38 µM) and β-pinene (binding constant of 14.51 µM). From this, we could infer that SsicOBP1 and SsicOBP2 are general odorant-binding proteins, which play an important role in sensing plant volatiles.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
1.60
自引率
0.00%
发文量
12
审稿时长
>12 weeks
期刊介绍: The Journal of the Kansas Entomological Society is a publication of the Kansas (Central States) Entomological Society, publishes research on all aspects of the sciences of entomology, and has world-wide authorship and readership.
期刊最新文献
Nanobiopesticides as an Alternative and Sustainable Solution to Tackle Pest Outbreaks Hedgerow Gardens Provide Floral Resources for Diverse Insect Visitors to Avocado Flowers in Southern California New Records of Gynandromorphism in Heriades and Dianthidium and Images of the First Reported Dianthidium Gynandromorph (Hymenoptera: Megachilidae) A Report of Bombyx mori Double Cocoon with Internal Eclosion, Mating and Oviposition Description of Larval Stage of Curculio mexicanus Gibson (Curculioninae; Curculionini) Associated with Several Species of Quercus (Fagaceae)
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1