胡椒干果水醇提取物对Wistar大鼠急性和亚急性毒性研究

IF 1.8 Q3 PHARMACOLOGY & PHARMACY Advances in Traditional Medicine Pub Date : 2023-03-31 DOI:10.1007/s13596-023-00680-8
Swati Vinod Jogdand, Ghanshyam B. Jadhav, Yogesh P. Talekar
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引用次数: 0

摘要

本研究通过对 Wistar 大鼠进行急性和亚急性口服给药,评估了瓜蒌干果水醇提取物(HEPL)的前瞻性毒性。在急性毒性研究中,雌性大鼠口服 HEPL 的单次剂量为 300 毫克/千克,重复剂量为 2000 毫克/千克(OECD 准则-423)。研究了 HEPL(250、500 和 1000 毫克/千克口服)的亚急性毒性,对照组(1% CMC)的 Wistar 雌雄大鼠每天服药 28 天。为评估可逆性,其他卫星组在治疗后再保留两周。急性毒性研究结果表明,无致死效应,毒性症状和半数致死剂量比 2000 毫克/千克更明显。在亚急性毒性研究中,大鼠口服提取物 28 天后,体重、食量和饮水量均无明显变化。此外,也没有观察到提取物对大鼠的血液学和生化指标产生毒性影响。组织病理学分析表明,大鼠的肾脏、睾丸和卵巢没有退化迹象,但肝脏出现轻度多灶性肝细胞坏死,并伴有炎性细胞浸润。这些结果表明,250 毫克/千克和 500 毫克/千克的剂量是安全的,此外,1000 毫克/千克的剂量也会对肝脏产生毒性。结论是 HEPL 的口服半数致死剂量比 2000 毫克/千克更显著,被认为是安全的。因此,胡椒龙葵的短期和长期口服剂量都是安全的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Acute and sub-acute toxicity studies of hydro-alcoholic extract of dried fruits of Piper longum Linn in Wistar rats

The current study evaluated the prospective toxicity of hydroalcoholic extract of Piper longum L. (HEPL) dried fruits with acute and sub-acute oral administration in Wistar rats. During acute toxicity study, female rats were orally administered with HEPL at a single dose of 300 mg/kg and repeated dose of 2000 mg/kg (OECD guidelines-423). Subacute toxicity of HEPL (250, 500, and 1000 mg/kg p.o.) was studied with the control group (1% CMC) by daily dosing in Wistar rats of both sexes for 28 days. To assess reversibility, other satellite groups were kept for another two weeks post-treatment. The acute toxicity study showed no lethal effects, and toxicity signs and LD50 transpire more significantly than 2000 mg/kg. In subacute toxicity study, oral administration of extract for 28 days; caused no significant changes in body weights; food and water consumption of rats were observed. In addition, no toxic effects of the extract on rats for hematological and biochemical parameters were observed. Histopathological analysis revealed no signs of degeneration for kidneys, testis, and ovaries; however liver showed mild multifocal hepatocellular necrosis with infiltration of inflammatory cells in rats treated with 1000 mg/kg of HEPL. These results showed that 250 mg/kg and 500 mg/kg were found safe, in addition to some liver toxicity was found for 1000 mg/kg. It is concluded that the oral LD50 of HEPL was more significant than 2000 mg/kg and is considered safe. Hence, Piper longum is safe in the short and long term for an oral dose.

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来源期刊
Advances in Traditional Medicine
Advances in Traditional Medicine PHARMACOLOGY & PHARMACY-
CiteScore
4.30
自引率
0.00%
发文量
50
期刊介绍: Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.
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