Study on the activity of Mori Fructus polysaccharides and its derivatives against acute alcoholic liver injury in mice

Four polysaccharides, MFPA1, MFPA2, MFPB1, and MFPB2, were isolated from Mori Fructus using DEAE-52 cellulose chromatography. MFPA1 (177 kDa) was composed of mannose, rhamnose, glucose, and xylose, and MFPB1 (165 kDa) was composed of mannose, rhamnose, galacturonic acid, glucose, and xylose, while MFPA2 (638 kDa) and MFPB2 (380 kDa) were consisted of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, xylose, and arabinose. These polysaccharides were sulfated to obtained Four sulfated polysaccharides, S-MFPA1, S-MFPA2, S-MFPB1, and S-MFPB2. The characteristic absorptive bands of purified polysaccharides and sulfated polysaccharides were determined by FT-IR. MFPA1, MFPB1, S-MFPA1, and S-MFPB1 showed excellent activities to activate alcohol dehydrogenase in vitro. Subsequently, it was found that MFPA1 had the strongest antiacute alcoholic liver injury activity through the experiments with acute alcoholic liver injury in mice. These results provide important scientific basis for Mori fructus polysaccharides as a potential therapeutic agent against acute alcoholic liver injury.