香蕉中维生素B6和B12的替代光学定性检测方法

I. Isnaeni, B. S. Zufara, I. Lewa
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引用次数: 0

摘要

众所周知,香蕉含有人体必需的纤维和维生素。因此,检测香蕉中这些维生素的能力是至关重要的。信息中的维生素含量可以影响采收程序和采收后的过程。测定食品营养成分的方法通常采用高效液相色谱法。然而,这种方法需要复杂的样品制备和化学反应过程。由于这一弱点,需要替代技术以简单的方式检测维生素。本研究建立了一种简便、快速测定香蕉中维生素含量的方法。利用反射光谱和光致发光光谱技术,鉴定了5种不同品种香蕉的维生素。从反射光谱结果可以观察到两个峰,在波长325 nm处的第一个峰是维生素B6的吸收峰,在波长450 nm处的第二个峰是维生素B12的吸收峰。在激发波长为325 nm的光致发光光谱中,在波长450 nm处发现了一个发光峰,这是维生素B6的发光峰。结果表明,本方法可简便、快捷地检测香蕉中维生素的含量。
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ALTERNATIVE OPTICAL METHODS FOR QUALITATIVE DETECTION OF VITAMIN B6 AND B12 OF BANANA
Bananas are known to contain fiber and vitamins essential for human body. Thus, the ability to detect these of vitamin in bananas is crucial. Information in the vitamin content of can affect procedures for harverst and post-harvest process. Methods to determine the nutrition content of foods are usually carried out using High Performance Liquid Chromatography (HPLC). However, this method requires complex sample preparation and chemical reaction processes. Due to this weakness, alternative techniques are needed to detect vitamin in simple ways. In this study, a simple, easy and fast methods to determine the vitamin content of banana was developed. Using reflectance and photoluminence spectroscopy, the vitamin of bananas from five different species were able to be identified. From the reflectance spectra results, two peaks were observed, the first peak at a wavelength of 325 nm is the absorption peak of vitamin B6 and the second peak at 450 nm is the absorption peak of vitamin B12. From the photoluminence spectra using excitation wavelength at 325 nm, an emission peak was found at wavelength 450 nm which is the peak emission from vitamin B6. These results proved that by using the methods proposed, the detection of vitamins in bananas can be done in an easy and simple ways.
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发文量
10
审稿时长
24 weeks
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