Li Xiaohua , Li Haitao , Jin Lingyu , Chen Leixi , Niu Yingfeng , Guan Yanhong , Ma Xiaojun , Zhang Lixia
{"title":"大白节根乙醇提取物乙酸乙酯部分:对A549癌细胞的抗肿瘤活性","authors":"Li Xiaohua , Li Haitao , Jin Lingyu , Chen Leixi , Niu Yingfeng , Guan Yanhong , Ma Xiaojun , Zhang Lixia","doi":"10.1016/S0254-6272(18)30905-1","DOIUrl":null,"url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To evaluate the anti-tumor activity of ethyl acetate fraction (EFA), extracted with ethanol from the root of “Dai-Bai-Jie” in A549 cancer cells and its underlying mechanism.</p></div><div><h3>METHODS</h3><p>“Dai-Bai-Jie” was extracted with 95% ethanol-aqueous (DBJ-1), 50% ethanol-aqueous (DBJ-2), and water (DBJ-3) by reflux method. 95% ethanol-aqueous extract was separated byethyl acetate (EFA) and n-butyl alcohol (DBJ-5), consecutively. The SRB method was used to evaluate the cytotoxic activity. Annexin V-FITC staining was applied to observe the apoptosis and analyze the cell cycle activated by EFA in A549 tumor cell. Western blot was used to detect the apoptosis/related proteins expressions. A549 tumor cellsbearing nude mice model was employed to measure the tumor volume, mice weight, and tumor inhibition ratio in order to verify the antitumor activity <em>in vivo.</em></p></div><div><h3>RESULTS</h3><p>DBJ-1 and EFA showed better cytotoxic activity on A549 tumor cells with IC<sub>50</sub> 25 and 3.5 µg/mL, respectively. EFA can exhibit the proliferation, arrest cell cycle at G0/G1 phase, and induce apoptosis in A549 tumor cells <em>in vitro.</em> The mechanisms of apoptosis induced by EFA may be associated with decreasing Bcl-2 protein expression and increasing p53, Bax, Caspase-3, and Caspase-8 proteins expression. EFA also possessed significant anti-tumor efficacy in nude mice, and little toxicity was observed in the host.</p></div><div><h3>CONCLUSION</h3><p>EAF could induce A549 tumor cells apoptosis and G0/G1 cell cycle arrest. A549 tumor cells apoptosis induced by EAF may be associated with the decrease in the ratio of Bcl-2 and Bax mRNA levels, and increase in the expression of p53, Caspase-3, and Caspase-8 proteins.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 668-675"},"PeriodicalIF":2.0000,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30905-1","citationCount":"1","resultStr":"{\"title\":\"Ethyl acetate fraction in ethanol extract from root of “Dai-Bai-Jie” (Marsdenia tenacissima): anti-tumor activity in A549 cancer cells\",\"authors\":\"Li Xiaohua , Li Haitao , Jin Lingyu , Chen Leixi , Niu Yingfeng , Guan Yanhong , Ma Xiaojun , Zhang Lixia\",\"doi\":\"10.1016/S0254-6272(18)30905-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>OBJECTIVE</h3><p>To evaluate the anti-tumor activity of ethyl acetate fraction (EFA), extracted with ethanol from the root of “Dai-Bai-Jie” in A549 cancer cells and its underlying mechanism.</p></div><div><h3>METHODS</h3><p>“Dai-Bai-Jie” was extracted with 95% ethanol-aqueous (DBJ-1), 50% ethanol-aqueous (DBJ-2), and water (DBJ-3) by reflux method. 95% ethanol-aqueous extract was separated byethyl acetate (EFA) and n-butyl alcohol (DBJ-5), consecutively. The SRB method was used to evaluate the cytotoxic activity. Annexin V-FITC staining was applied to observe the apoptosis and analyze the cell cycle activated by EFA in A549 tumor cell. Western blot was used to detect the apoptosis/related proteins expressions. A549 tumor cellsbearing nude mice model was employed to measure the tumor volume, mice weight, and tumor inhibition ratio in order to verify the antitumor activity <em>in vivo.</em></p></div><div><h3>RESULTS</h3><p>DBJ-1 and EFA showed better cytotoxic activity on A549 tumor cells with IC<sub>50</sub> 25 and 3.5 µg/mL, respectively. EFA can exhibit the proliferation, arrest cell cycle at G0/G1 phase, and induce apoptosis in A549 tumor cells <em>in vitro.</em> The mechanisms of apoptosis induced by EFA may be associated with decreasing Bcl-2 protein expression and increasing p53, Bax, Caspase-3, and Caspase-8 proteins expression. EFA also possessed significant anti-tumor efficacy in nude mice, and little toxicity was observed in the host.</p></div><div><h3>CONCLUSION</h3><p>EAF could induce A549 tumor cells apoptosis and G0/G1 cell cycle arrest. A549 tumor cells apoptosis induced by EAF may be associated with the decrease in the ratio of Bcl-2 and Bax mRNA levels, and increase in the expression of p53, Caspase-3, and Caspase-8 proteins.</p></div>\",\"PeriodicalId\":17513,\"journal\":{\"name\":\"Journal of Traditional Chinese Medicine\",\"volume\":\"38 5\",\"pages\":\"Pages 668-675\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2018-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30905-1\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Traditional Chinese Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0254627218309051\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Traditional Chinese Medicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0254627218309051","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
Ethyl acetate fraction in ethanol extract from root of “Dai-Bai-Jie” (Marsdenia tenacissima): anti-tumor activity in A549 cancer cells
OBJECTIVE
To evaluate the anti-tumor activity of ethyl acetate fraction (EFA), extracted with ethanol from the root of “Dai-Bai-Jie” in A549 cancer cells and its underlying mechanism.
METHODS
“Dai-Bai-Jie” was extracted with 95% ethanol-aqueous (DBJ-1), 50% ethanol-aqueous (DBJ-2), and water (DBJ-3) by reflux method. 95% ethanol-aqueous extract was separated byethyl acetate (EFA) and n-butyl alcohol (DBJ-5), consecutively. The SRB method was used to evaluate the cytotoxic activity. Annexin V-FITC staining was applied to observe the apoptosis and analyze the cell cycle activated by EFA in A549 tumor cell. Western blot was used to detect the apoptosis/related proteins expressions. A549 tumor cellsbearing nude mice model was employed to measure the tumor volume, mice weight, and tumor inhibition ratio in order to verify the antitumor activity in vivo.
RESULTS
DBJ-1 and EFA showed better cytotoxic activity on A549 tumor cells with IC50 25 and 3.5 µg/mL, respectively. EFA can exhibit the proliferation, arrest cell cycle at G0/G1 phase, and induce apoptosis in A549 tumor cells in vitro. The mechanisms of apoptosis induced by EFA may be associated with decreasing Bcl-2 protein expression and increasing p53, Bax, Caspase-3, and Caspase-8 proteins expression. EFA also possessed significant anti-tumor efficacy in nude mice, and little toxicity was observed in the host.
CONCLUSION
EAF could induce A549 tumor cells apoptosis and G0/G1 cell cycle arrest. A549 tumor cells apoptosis induced by EAF may be associated with the decrease in the ratio of Bcl-2 and Bax mRNA levels, and increase in the expression of p53, Caspase-3, and Caspase-8 proteins.
期刊介绍:
Journal of Traditional Chinese Medicine(JTCM) is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.