{"title":"HPLC-MS/MS测定尿肌酐的新方法:与Jaffè方法的比较研究","authors":"Lidia Caporossi, Enrico Paci, Silvia Capanna, Bruno Papaleo, Giovanna Tranfo, Daniela Pigini","doi":"10.1016/j.urine.2023.04.002","DOIUrl":null,"url":null,"abstract":"<div><p>Urinary biomarkers are usually reported as a normalized ratio to creatinuria, a lack of correct creatinine determination lead to wrong final data. This study aimed to evaluate the Jaffè method accuracy, proposing a new HPLC-MS/MS method. The validation parameters were: run time of 4 min, LOD of 0.02 mg/L, CV% lower than 4%, recovery >96.1%, no matrix effect.</p><p>The comparison between HPLC-MS/MS and Jaffè method showed an average bias, variable from high (1.5–3.00 g/L) to low concentrations (below than 0.7 g/L) of creatinine, ranging from +31% to −25%. The critical issue for the normalization of biomonitoring data was highlighted.</p></div>","PeriodicalId":75287,"journal":{"name":"Urine (Amsterdam, Netherlands)","volume":"5 ","pages":"Pages 23-28"},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"A new HPLC-MS/MS method for urinary creatinine determination: Comparison study with Jaffè’s method\",\"authors\":\"Lidia Caporossi, Enrico Paci, Silvia Capanna, Bruno Papaleo, Giovanna Tranfo, Daniela Pigini\",\"doi\":\"10.1016/j.urine.2023.04.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Urinary biomarkers are usually reported as a normalized ratio to creatinuria, a lack of correct creatinine determination lead to wrong final data. This study aimed to evaluate the Jaffè method accuracy, proposing a new HPLC-MS/MS method. The validation parameters were: run time of 4 min, LOD of 0.02 mg/L, CV% lower than 4%, recovery >96.1%, no matrix effect.</p><p>The comparison between HPLC-MS/MS and Jaffè method showed an average bias, variable from high (1.5–3.00 g/L) to low concentrations (below than 0.7 g/L) of creatinine, ranging from +31% to −25%. The critical issue for the normalization of biomonitoring data was highlighted.</p></div>\",\"PeriodicalId\":75287,\"journal\":{\"name\":\"Urine (Amsterdam, Netherlands)\",\"volume\":\"5 \",\"pages\":\"Pages 23-28\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Urine (Amsterdam, Netherlands)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2590280623000037\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Urine (Amsterdam, Netherlands)","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590280623000037","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A new HPLC-MS/MS method for urinary creatinine determination: Comparison study with Jaffè’s method
Urinary biomarkers are usually reported as a normalized ratio to creatinuria, a lack of correct creatinine determination lead to wrong final data. This study aimed to evaluate the Jaffè method accuracy, proposing a new HPLC-MS/MS method. The validation parameters were: run time of 4 min, LOD of 0.02 mg/L, CV% lower than 4%, recovery >96.1%, no matrix effect.
The comparison between HPLC-MS/MS and Jaffè method showed an average bias, variable from high (1.5–3.00 g/L) to low concentrations (below than 0.7 g/L) of creatinine, ranging from +31% to −25%. The critical issue for the normalization of biomonitoring data was highlighted.
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