肉制品中单核细胞增生性李斯特菌的检测及基因测序方法综述

B. Setiani, Y. B. Pramono, L. Purwitasari
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引用次数: 0

摘要

对致病菌单核细胞增多性李斯特菌、从肉制品中分离的检测和基因测序方法进行了综述,比较了检测所选生加工肉制品中单核细胞增生性李斯特菌的方法。结果表明,单核细胞增多李斯特菌(原名单核细胞增生杆菌)是一种革兰氏阳性、非孢子转化、高流动性、杆状兼性厌氧菌。它可以在-1.5°C至45°C的温度下生长,在4.4至9.4的pH范围内生长,最适pH为7。快速方法(基于PCR和VIDAS-LDUO®)比传统方法更快地检测出单核细胞增多性李斯特菌。表型鉴定和基因型鉴定是单核细胞增多性李斯特菌的两种鉴定方法。由于环境污染、交叉污染或工艺错误,在生肉和肉制品中可发现单核细胞增多性李斯特菌。
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Review on Pathogenic Bacteria Listeria monocytogenes, The Detection and The Sequencing Gene Methods Isolated from Meat Products
A study was conducted to review on pathogenic bacteria Listeria monocytogenes, the detection and the sequencing gene methods isolated from meat products, compare selected methods that detect the presence of Listeria monocytogenes in selected raw and processed meat products. Results indicate that Listeria monocytogenenes (originally named Bacterium monocytogenes) is a gram-positive, non-sporeforming, highly mobile, rod-type, and facultative anaerobic bacterium species. It can grow under temperatures between -1.5°C to 45°C and at pH range between 4.4 and 9.4, with the optimum pH of 7. Rapid methods (PCR based and VIDAS-LDUO®) detected Listeria monocytogenes faster than the conventional method. It was also gathered that Phenotypic identification and Genotypic identification are two types of confirmation test for Listeria monocytogenes. Listeria monocytogenenes can be found in raw meat and meat product because of environmental contamination, cross contamination or error process.
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