Monika Ňorbová, A. Vollmannová, Eduard Pintér, Marek Šnirc, H. Franková, Silvia Fedorková, Natália Čeryová
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引用次数: 0
摘要
本研究旨在测定不同类型果实的维生素C含量和抗氧化活性:山茱萸(Cornus mas L.)、服务树(Sorbus domestica)、枸杞(Mespilus germanica L.)、木瓜(Asimina triloba L.)、柿子(Diospyros kaki L.)。通过HPLC系统Waters Separations Module 2695和UV检测器2996测定水果的维生素C含量。维生素C的含量达到72.238µg。g-1(Diospyros kaki L.)至1114.61µg.g-1(Sorbus domestica)。采用分光光度法测定其抗氧化活性(DPPH)。DPPH自由基清除活性值为2.28(德国麦穗)-12.34μmol TE.g-1 DW(美国玉米)。Spearman试验显示两个监测参数之间呈正相关。研究物种的维生素C含量与抗氧化活性呈正相关。由于维生素C和抗氧化活性含量高,未来的分析应侧重于测定这些水果中的其他生物活性物质。
THE CONTENT OF VITAMIN C AND ANTIOXIDANT ACTIVITY IN LESS-KNOWN TYPES OF FRUIT
This study aims to determine the vitamin C content and antioxidant activity in different types of fruit: cornelian cherry (Cornus mas L.), service tree (Sorbus domestica), medlar (Mespilus germanica L.), pawpaw (Asimina triloba L.), persimmon (Diospyros kaki L.). The aim of the work was to compare these used methods. The fruit’s vitamin C content was determined by HPLC system Waters Separations Module 2695 with UV detector 2996. The content of vitamin C reached values from 72.238 µg. g-1 (Diospyros kaki L.) to 1114.61 µg.g-1 (Sorbus domestica). The antioxidant activity (DPPH) was determined spectrophotometrically. DPPH radical scavenging activity values were 2.28 (Mespilus germanica L.) – 12.34 μmol TE.g-1 DW (Cornus mas L.). Spearman's test showed a positive relationship between both monitored parameters. The content of vitamin C in the studied species showed a positive relationship with antioxidant activity. Due to the high content of vitamin C and antioxidant activity, future analyses should focus on the determination of other bioactive substances in these fruits.
期刊介绍:
The Journal of Microbiology, Biotechnology and Food Sciences is an Open Access, peer-reviewed online scientific journal published by the Faculty of Biotechnology and Food Sciences (Slovak University of Agriculture in Nitra). The major focus of the journal is regular publishing of original scientific articles, short communications and reviews about animal, plant and environmental microbiology (including bacteria, fungi, yeasts, algae, protozoa and viruses), microbial, animal and plant biotechnology and physiology, microbial, plant and animal genetics, molecular biology, agriculture and food chemistry and biochemistry, food control, evaluation and processing in food science and environmental sciences.