光谱法测定乙酰胆碱(ACh)的研究进展

IF 7.1 2区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Topics in Current Chemistry Pub Date : 2023-05-11 DOI:10.1007/s41061-023-00426-9
Paweł Świt, Aleksandra Pollap, Joanna Orzeł
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引用次数: 1

摘要

乙酰胆碱(ACh)是在脊椎动物和无脊椎动物中发现的胆碱能系统中最重要的神经递质之一,负责生物体的许多过程。乙酰胆碱传递障碍与阿尔茨海默病和帕金森病的痴呆密切相关。生物样品中的乙酰胆碱通常使用色谱技术、放射酶测定、酶联免疫吸附测定(ELISA)或电位测定法测定。另一种检测和测定乙酰胆碱的方法是应用光谱技术,由于检测和定量限低,这是不可能用上述方法。在这篇综述文章中,我们详细概述了不同的光谱方法,通过一系列验证参数来确定乙酰胆碱,作为常规分析的透视工具,特别是在中枢神经系统动物模型的基础研究中。此外,还讨论了其他生物材料的临床和临床前研究实例,给出了全光谱光谱方法的应用。介绍了化学传感器的发展情况,以及流动技术的应用。值得强调的是,文章中包含了涉及其他神经递质的多组分分析,以及对测试生物样品和提取程序的描述。本文简要讨论了利用光谱技术进行这类分析的动机和该领域的未来前景。图形抽象
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Spectroscopic Determination of Acetylcholine (ACh): A Representative Review

Acetylcholine (ACh) is one of the most crucial neurotransmitters of the cholinergic system found in vertebrates and invertebrates and is responsible for many processes in living organisms. Disturbances in ACh transmission are closely related to dementia in Alzheimer’s and Parkinson’s disease. ACh in biological samples is most often determined using chromatographic techniques, radioenzymatic assays, enzyme-linked immunosorbent assay (ELISA), or potentiometric methods. An alternative way to detect and determine acetylcholine is applying spectroscopic techniques, due to low limits of detection and quantification, which is not possible with the methods mentioned above. In this review article, we described a detailed overview of different spectroscopic methods used to determine ACh with a collection of validation parameters as a perspective tool for routine analysis, especially in basic research on animal models on central nervous system. In addition, there is a discussion of examples of other biological materials from clinical and preclinical studies to give the whole spectrum of spectroscopic methods application. Descriptions of the developed chemical sensors, as well as the use of flow technology, were also presented. It is worth emphasizing the inclusion in the article of multi-component analysis referring to other neurotransmitters, as well as the description of the tested biological samples and extraction procedures. The motivation to use spectroscopic techniques to conduct this type of analysis and future perspectives in this field are briefly discussed.

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来源期刊
Topics in Current Chemistry
Topics in Current Chemistry Chemistry-General Chemistry
CiteScore
13.70
自引率
1.20%
发文量
48
期刊介绍: Topics in Current Chemistry is a journal that presents critical reviews of present and future trends in modern chemical research. It covers all areas of chemical science, including interactions with related disciplines like biology, medicine, physics, and materials science. The articles in this journal are organized into thematic collections, offering a comprehensive perspective on emerging research to non-specialist readers in academia or industry. Each review article focuses on one aspect of the topic and provides a critical survey, placing it in the context of the collection. Selected examples highlight significant developments from the past 5 to 10 years. Instead of providing an exhaustive summary or extensive data, the articles concentrate on methodological thinking. This approach allows non-specialist readers to understand the information fully and presents the potential prospects for future developments.
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