{"title":"二甲双胍对实验性骨关节炎大鼠保护作用的评价","authors":"Ali K. Al-buhadily, Rabab Al-Uqabi, A. Al-Gareeb","doi":"10.4103/mj.mj_43_22","DOIUrl":null,"url":null,"abstract":"Objective: The objective of this study is to evaluate metformin proposed protective effect against the development of osteoarthritis (OA) in rats. Materials and Methods: A total of 40 male Sprague–Dawley rats were included, divided into four groups: negative control (n = 10), positive control (OA induced by monoiodoacetate [MIA]) (n = 10), metformin 200mg/kg group (n = 10) preinduction of OA, and metformin 200 mg/kg group (n = 10) postinduction of OA. Serum C-telopeptide type II collagen (CTX-II), inflammatory biomarkers were evaluated for each group. For data analysis, SPSS version 26.00 was used. Results: Metformin-treated groups showed a significant reduction in inflammatory biomarkers and CTX-II serum levels compared with OA group (P < 0.05). Pretreatment with 200 mg/kg metformin imparted extra cartilage protective effect and further decreased inflammatory cytokines compared to posttreated (P < 0.05). Conclusion: Metformin produced a beneficial protective effect in experimental OA in rats. It attenuates the inflammatory reactions progression by preventing the release of pro-inflammatory cytokines in rats with experimental OA. Furthermore, metformin reduced cartilage degradation evidenced by lowering CTX-II serum levels experimental OA in rats.","PeriodicalId":33069,"journal":{"name":"mjl@ lmstnSry@ lTby@","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of protective effect of metformin in rats with experimental osteoarthritis\",\"authors\":\"Ali K. Al-buhadily, Rabab Al-Uqabi, A. Al-Gareeb\",\"doi\":\"10.4103/mj.mj_43_22\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective: The objective of this study is to evaluate metformin proposed protective effect against the development of osteoarthritis (OA) in rats. Materials and Methods: A total of 40 male Sprague–Dawley rats were included, divided into four groups: negative control (n = 10), positive control (OA induced by monoiodoacetate [MIA]) (n = 10), metformin 200mg/kg group (n = 10) preinduction of OA, and metformin 200 mg/kg group (n = 10) postinduction of OA. Serum C-telopeptide type II collagen (CTX-II), inflammatory biomarkers were evaluated for each group. For data analysis, SPSS version 26.00 was used. Results: Metformin-treated groups showed a significant reduction in inflammatory biomarkers and CTX-II serum levels compared with OA group (P < 0.05). Pretreatment with 200 mg/kg metformin imparted extra cartilage protective effect and further decreased inflammatory cytokines compared to posttreated (P < 0.05). Conclusion: Metformin produced a beneficial protective effect in experimental OA in rats. It attenuates the inflammatory reactions progression by preventing the release of pro-inflammatory cytokines in rats with experimental OA. Furthermore, metformin reduced cartilage degradation evidenced by lowering CTX-II serum levels experimental OA in rats.\",\"PeriodicalId\":33069,\"journal\":{\"name\":\"mjl@ lmstnSry@ lTby@\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"mjl@ lmstnSry@ lTby@\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/mj.mj_43_22\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"mjl@ lmstnSry@ lTby@","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/mj.mj_43_22","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Evaluation of protective effect of metformin in rats with experimental osteoarthritis
Objective: The objective of this study is to evaluate metformin proposed protective effect against the development of osteoarthritis (OA) in rats. Materials and Methods: A total of 40 male Sprague–Dawley rats were included, divided into four groups: negative control (n = 10), positive control (OA induced by monoiodoacetate [MIA]) (n = 10), metformin 200mg/kg group (n = 10) preinduction of OA, and metformin 200 mg/kg group (n = 10) postinduction of OA. Serum C-telopeptide type II collagen (CTX-II), inflammatory biomarkers were evaluated for each group. For data analysis, SPSS version 26.00 was used. Results: Metformin-treated groups showed a significant reduction in inflammatory biomarkers and CTX-II serum levels compared with OA group (P < 0.05). Pretreatment with 200 mg/kg metformin imparted extra cartilage protective effect and further decreased inflammatory cytokines compared to posttreated (P < 0.05). Conclusion: Metformin produced a beneficial protective effect in experimental OA in rats. It attenuates the inflammatory reactions progression by preventing the release of pro-inflammatory cytokines in rats with experimental OA. Furthermore, metformin reduced cartilage degradation evidenced by lowering CTX-II serum levels experimental OA in rats.