龙葵通过TLR4介导的JNK活化诱导巨噬细胞活化，并通过TLR4中介导的p38和JNK活化介导巨噬细胞自噬

IF 1.7 3区农林科学 Q3 CHEMISTRY, APPLIED Food and Agricultural Immunology Pub Date : 2022-08-16 DOI:10.1080/09540105.2022.2113507

Julia Yu, Min Yeong Choi, So-Jung Park, Na Gyeong Geum, Jae Won Lee, G. Park, H. Eo, J. Jeong

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引用次数: 0

摘要

摘要在本研究中，我们研究了龙葵地上部分（SNAP）对RAW264.7细胞巨噬细胞活化和巨噬细胞自噬的影响。SNAP增加了RAW264.7细胞中免疫刺激因子的产生和吞噬作用。TLR4抑制阻断SNAP介导的免疫刺激因子的产生。此外，JNK抑制降低了SNAP介导的免疫刺激因子的产生，并且SNAP介介导的JNK激活被TLR4抑制所阻断。SNAP激活巨噬细胞自噬。TLR4的抑制阻断了SNAP介导的巨噬细胞自噬，p38和JNK的抑制减弱了SNAP中介导的巨噬细胞自噬。这些发现表明SNAP可能诱导TLR4/JNK介导的巨噬细胞活化以及TLR4/p38和JNK介导的细胞自噬。

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Solanum nigrum induces macrophage activation through TLR4-mediated activation of JNK and macrophage autophagy through TLR4-mediated activation of p38 and JNK

ABSTRACT In this study, we investigated the effect of Solanum nigrum aerial parts (SNAP) on macrophage activation and macrophage autophagy in RAW264.7 cells. SNAP increased the production of immunostimulatory factors and phagocytosis in RAW264.7 cells. TLR4 inhibition blocked SNAP-mediated production of immunostimulatory factors. In addition, the JNK inhibition reduced the SNAP-mediated production of immunostimulatory factors, and the SNAP-mediated JNK activation was blocked by the TLR4 inhibition. SNAP activated macrophage autophagy. TLR4 inhibition blocked SNAP-mediated macrophage autophagy and inhibition of p38 and JNK attenuated SNAP-mediated macrophage autophagy. These findings indicate that SNAP may induce TLR4/JNK-mediated macrophage activation and TLR4/p38 and JNK-mediated macrophage autophagy.

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来源期刊

Food and Agricultural Immunology 农林科学-毒理学

CiteScore

5.30

自引率

6.70%

发文量

审稿时长

2 months

期刊介绍： Food and Agricultural Immunology is an international open access journal publishing original immunological research with applications in food, agricultural, environmental and veterinary science. Submissions describing the use of immunological techniques and methods are particularly welcomed. The journal aims to expand our understanding of the interactions at the interface of food and immune systems including studies on: -Development of diagnostic systems – all types of ligand-based assays, e.g. antibody, aptamer -Application of ligand-based assays for the detection or identification of molecules of interest in food science, agricultural research, veterinary investigations and clinical systems relating to food allergy or sensitivity to agricultural chemicals -Effects of food on the immune system -Studies on allergy and allergic reactions -Investigations into food allergies -Development of allergen-free food systems -Development of novel assay formats -Applications of assay systems to the monitoring of food items in relation to safety and labelling -Food quality issues, e.g. speciation, adulteration and contamination -Comparisons between different analytical techniques The journal publishes research and review articles and is essential reading for food scientists, immunologists and all those concerned with the interaction between food and immune systems.