时间压缩超分辨率显微镜,帧率为1200帧/秒,空间分辨率为100纳米

IF 20.6 1区 物理与天体物理 Q1 OPTICS Advanced Photonics Pub Date : 2023-03-01 DOI:10.1117/1.AP.5.2.026003
Yilin He, Yunhua Yao, D. Qi, Yuping He, Zhen-Jian Huang, Pengpeng Ding, C. Jin, Chonglei Zhang, L. Deng, K. Shi, Zhenrong Sun, Xiaocong Yuan, Shian Zhang
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引用次数: 0

摘要

摘要各种超分辨率显微技术已经被提出来探索生物标本的精细结构。然而,通过点扫描或多帧计算,通常以降低成像速度为代价来获得超分辨率能力。空间分辨率与成像速度之间的矛盾严重阻碍了精细结构高速动力学的观测。为了克服这一矛盾,我们提出并演示了一种时间压缩超分辨率显微镜(TCSRM)技术。该技术是将增强时间压缩显微镜与基于深度学习的超分辨率图像重建相融合,利用增强时间压缩显微镜提高成像速度,利用基于深度学习的超分辨率图像重建实现分辨率增强。通过在微流控芯片上捕获流动的荧光珠,实验证明了TCSRM具有1200帧/秒、100nm空间分辨率的高速超分辨率成像能力。由于具有高速超分辨率的优异成像性能,TCSRM为精细结构,特别是生物医学领域的高速动力学行为研究提供了理想的工具。
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Temporal compressive super-resolution microscopy at frame rate of 1200 frames per second and spatial resolution of 100 nm
Abstract. Various super-resolution microscopy techniques have been presented to explore fine structures of biological specimens. However, the super-resolution capability is often achieved at the expense of reducing imaging speed by either point scanning or multiframe computation. The contradiction between spatial resolution and imaging speed seriously hampers the observation of high-speed dynamics of fine structures. To overcome this contradiction, here we propose and demonstrate a temporal compressive super-resolution microscopy (TCSRM) technique. This technique is to merge an enhanced temporal compressive microscopy and a deep-learning-based super-resolution image reconstruction, where the enhanced temporal compressive microscopy is utilized to improve the imaging speed, and the deep-learning-based super-resolution image reconstruction is used to realize the resolution enhancement. The high-speed super-resolution imaging ability of TCSRM with a frame rate of 1200 frames per second (fps) and spatial resolution of 100 nm is experimentally demonstrated by capturing the flowing fluorescent beads in microfluidic chip. Given the outstanding imaging performance with high-speed super-resolution, TCSRM provides a desired tool for the studies of high-speed dynamical behaviors in fine structures, especially in the biomedical field.
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来源期刊
CiteScore
22.70
自引率
1.20%
发文量
49
审稿时长
18 weeks
期刊介绍: Advanced Photonics is a highly selective, open-access, international journal that publishes innovative research in all areas of optics and photonics, including fundamental and applied research. The journal publishes top-quality original papers, letters, and review articles, reflecting significant advances and breakthroughs in theoretical and experimental research and novel applications with considerable potential. The journal seeks high-quality, high-impact articles across the entire spectrum of optics, photonics, and related fields with specific emphasis on the following acceptance criteria: -New concepts in terms of fundamental research with great impact and significance -State-of-the-art technologies in terms of novel methods for important applications -Reviews of recent major advances and discoveries and state-of-the-art benchmarking. The journal also publishes news and commentaries highlighting scientific and technological discoveries, breakthroughs, and achievements in optics, photonics, and related fields.
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