Humicola鞋垫角质酶催化聚对苯二甲酸乙二醇酯和AkestraTM解聚的比较

IF 2.5 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Frontiers in chemical engineering Pub Date : 2022-12-13 DOI:10.3389/fceng.2022.1048744
Lucía Aristizábal-Lanza, Smita V. Mankar, Cecilia Tullberg, Baozhong Zhang, Javier A. Linares-Pastén
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引用次数: 3

摘要

合成聚酯的酶解聚合已成为塑料回收研究的热点。由于聚对苯二甲酸乙二醇酯(PET)的广泛应用,该领域的大部分研究都集中在其解聚上。然而,对其他商业聚酯的酶活性研究较少。因此,AkestraTM引起了我们的注意,这是一种由PET衍生的具有部分生物基螺旋环缩醛结构的共聚物。本研究研究了葎草枯皮酶(HiCut)在PET和AkestraTM薄膜和粉末上的活性。HiCut在非晶PET薄膜上的解聚活性高于在Akestra™薄膜上的解聚活性。然而,AkestraTM粉体在235h内解聚率达到38%,而PET粉体的解聚率仅为12%。这些结果与酶对聚合物结晶度的依赖性一致,因为Akestra™是无定形的,而PET粉末的结晶度为14%。另一方面,HiCut对接研究和分子动力学模拟(MD)表明pet衍生的单(羟乙基)对苯二甲酸二聚体(MHET)2是一种可水解的配体,产生对苯二甲酸(TPA),而Akestra™衍生的TPA-螺二醇酯不是,这与实验确定的解聚产物一致。MD研究也提示配体诱导活性部位局部构象改变。
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Comparison of the enzymatic depolymerization of polyethylene terephthalate and AkestraTM using Humicola insolens cutinase
The enzymatic depolymerization of synthetic polyesters has become of great interest in recycling plastics. Most of the research in this area focuses on the depolymerization of polyethylene terephthalate (PET) due to its widespread use in various applications. However, the enzymatic activity on other commercial polyesters is less frequently investigated. Therefore, AkestraTM attracted our attention, which is a copolymer derived from PET with a partially biobased spirocyclic acetal structure. In this study, the activity of Humicola insolens cutinase (HiCut) on PET and AkestraTM films and powder was investigated. HiCut showed higher depolymerization activity on amorphous PET films than on Akestra™ films. However, an outstanding performance was achieved on AkestraTM powder, reaching 38% depolymerization in 235h, while only 12% for PET powder. These results are consistent with the dependence of the enzymes on the crystallinity of the polymer since Akestra™ is amorphous while the PET powder has 14% crystallinity. On the other hand, HiCut docking studies and molecular dynamic simulations (MD) suggested that the PET-derived mono (hydroxyethyl)terephthalate dimer (MHET)2 is a hydrolyzable ligand, producing terephthalic acid (TPA), while the Akestra™-derived TPA-spiroglycol ester is not, which is consistent with the depolymerization products determined experimentally. MD studies also suggest ligand-induced local conformational changes in the active site.
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CiteScore
3.50
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审稿时长
13 weeks
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