骨髓间充质干细胞抑制膝关节炎大鼠炎症并促进软骨细胞活性

Hua Huang, Yang‐xiong Zhu, Sining Li
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摘要

本研究旨在评估骨髓间充质干细胞是否抑制炎症并促进膝关节关节炎的软骨细胞活性。将36只SD大鼠随机分为H组、K组、M组和B组,分别建立膝关节关节炎大鼠模型。造模后,B组大鼠输注骨髓间充质干细胞,M组大鼠输注甲氨蝶呤6周,分析TNF-α、IL-6、IL-1水平、膝关节软骨形态、软骨细胞活性及NO、ERα、cGMP蛋白表达。H&E染色发现H组膝关节软骨表面光滑,软骨细胞形态正常。K组关节软骨表面出现骨裂,深部细胞增生紊乱。B、M组关节软骨表面逐渐光滑。与H组比较,K组TNF-α、IL-6、IL-1水平升高(P < 0.05);M组和B组均较K组降低(P < 0.05)。与K组比较,H、M、B组软骨细胞活性均升高(P < 0.05)。K组膝关节软骨组织NO、ERα和cGMP水平较H组降低(P < 0.05), M组和B组升高(P < 0.05)。综上所述,BMSCs可下调IL-6、IL-1、TNF-α,增强软骨细胞活性,上调NO、ERα、cGMP水平,为治疗膝关节关节炎提供了新的思路。
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Bone Marrow Mesenchymal Stem Cells Inhibits Inflammation and Promotes Chondrocyte Activity in Knee Arthritis Rats
This study intends to assess whether BMSCs inhibits inflammation and promotes chondrocyte activity in knee arthritis. 36 SD rats were randomly assigned into group H, group K, group M and group B. The rat models of knee arthritis were established in Group K, group M and group B. After modeling, BMSCs were infused into rats in group B and methotrexate to rats in group M for 6 weeks followed by analysis of TNF-α, IL-6 and IL-1 levels, morphology of knee cartilage, chondrocyte activity and the expression of NO, ERα and cGMP protein. H&E staining found that the surface of knee cartilage in group H was smooth and the morphology of chondrocytes was normal. In group K, bone fissure was formed on articular cartilage surface, and the hyperplasia of deep cells was disorder. The surface of articular cartilage in group B and M gradually became smooth. Compared to group H, TNF-α, IL-6 and IL-1 levels in group K were increased (P < 0.05); their levels in group M and group B were decreased relative to K group (P < 0.05). Compared to group K, chondrocytes activity in groups H, M and B was increased (P < 0.05). NO, ERα and cGMP levels were decreased in knee cartilage of group K relative to H group (P < 0.05) and increased in group M and group B (P < 0.05). In conclusion, BMSCs can down-regulate IL-6, IL-1 and TNF-α, enhance chondrocytes activity, and up-regulate the levels of NO, ERα and cGMP, thus providing a new idea for treating knee arthritis.
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