高效薄层色谱-质谱法评价食用芥菜油中墨西哥银皂籽中血根碱和二氢血根碱的含量

Ramakant Yadav
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引用次数: 0

摘要

本研究建立了一种简便、快速、廉价、灵敏、重现性好的高效液相色谱-质谱联用方法,用于鉴定墨西哥银Argemone mexicana Linn种子中两种重要的生物活性成分血根碱和双氢血根碱。本工作进一步讨论并建立了一种灵敏的HPTLC - MS方法,以血根碱和双氢血根碱为生物标志物,分析食用芥菜油中亚银酮油的掺假和/或污染。以正己烷:乙醚(1:1 v/v)溶剂体系为萃取介质,从种子中提取血根碱和二氢血根碱,并进行HPLC-MS检测。CAMAG HPTLC系统模块由ATS-4、ADC-2、可视化器-2、TLC扫描仪-4组成;衍生剂和TLC-MS接口-2分别用于样品应用、HPTLC板显影、板照片记录、板扫描、衍生试剂喷涂和直接从HPTLC板上洗脱生物标志物。正己烷:丙酮(23:7,v/v)和dragendorff试剂分别作为流动相和衍生化试剂。紫外光谱和质谱数据证实在样品/加标样品中检测到选定的生物标志物。因此,本工作强调利用HPTLC-MS建立一种简便、常规、灵敏的检测墨西哥银荷种子中血碱和双氢血碱的方法,并可为其他样品(如各种食用油、农产品、药物和生物医药产品等)中这些生物标志物的评价提供基础。
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High Performance Thin-Layer Chromatography-Mass Spectrometry Evaluation of Sanguinarine and Dihydrosanguinarine from Argemone mexicana Seeds in Edible Mustard Oil
In this present study, a simple, rapid, cheap, sensitive and reproducible HPTLC-MS Method has been developed for the identification of two important bioactive compounds, Sanguinarine and Dihydrosanguinarine in Argemone mexicana Linn seeds. The work further discussed and developed a sensitive HPTLC – MS method to analyse the adulteration and/or contamination of argemone oil in the edible mustard oil by spiking Sanguinarine and Dihydrosanguinarine as biomarkers. The n-hexane: diethyl ether (1:1 v/v) solvent system has been used as an extraction medium to extract the Sanguinarine and Dihydrosanguinarine from the seeds followed by HPLC-MS detection.The CAMAG HPTLC system modules consisted with ATS-4, ADC-2, visualizer-2, TLC scanner-4; Derivatizer and TLC-MS interface-2 have been used for sample application, HPTLC plate development, plate photo documentation, scanning of plate, spraying of derivatization reagent and elution of biomarkers directly from HPTLC plate respectively. The n-hexane: acetone (23:7, v/v) and dragendorff’s reagent has been used as mobile phase and derivatization reagent respectively. The UV- spectra and MS data confirmed the detection of selected biomarkers in the samples/spiked samples. Thus, the work highlights the use of HPTLC-MS to develop simple and routine method for the sensitive detection of Sanguinarine and Dihydrosanguinarine in Argemone mexicana seeds and able to become bases to these biomarkers’ evaluations in other samples such as various edible oils, agro-products, drugs and biomedical products etc.
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Bioscience Biotechnology Research Communications
Bioscience Biotechnology Research Communications BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
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