肉桂叶精油提取工艺的优化研究

IF 2.3 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Applied Biological Chemistry Pub Date : 2023-08-19 DOI:10.1186/s13765-023-00798-y
Reta Merid Yitbarek, Habtamu Admassu, Fekiya Mohammed Idris, Eskindir Getachew Fentie
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引用次数: 0

摘要

蒸汽蒸馏法提取肉桂叶精油耗时耗力。此外,较低的收率和较高的产品降解率是这种方法的主要缺点。因此,本研究的目的是利用响应面法优化肉桂叶精油的提取工艺参数,以最大限度地提高肉桂叶精油的收率,同时保证肉桂叶精油的质量。本研究的另一个目的是将提取的精油应用于碎牛肉上,以评估其防腐效果。选取提取时间(120 ~ 210 min)、提取温度(105 ~ 115℃)和进料质量(300 ~ 600 g)为自变量,采用中心复合设计(CCD)进行优化实验。此外,还对提取的精油对肉糜的抑菌和微生物防腐活性进行了评价。在提取时间为175.43 min、提取温度为105℃、饲料质量为600 g的条件下,肉桂叶精油得率和肉桂醛浓度(%面积)的最佳预测值分别为2.9%和34.6%。二阶多项式方程与20次运行的实验数据拟合。最佳提取条件下肉桂叶精油的化学成分以丁香酚(60.68%)和肉桂醛(33.94%)为主。此外,最佳提取肉桂精油抑制细菌的生长,特别是革兰氏阳性细菌。在4℃条件下贮存21 d后,肉桂精油浓度为1.2% (v/v)的牛肉肉糜的总活菌数低于106 CFU/g。综上所述,优化后的肉桂叶精油提取工艺在保留肉桂叶精油功能特性的同时,获得了较高的得率。
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Optimizing the extraction of essential oil from cinnamon leaf (Cinnamomum verum) for use as a potential preservative for minced beef

Cinnamon leaf essential oil extraction using steam distillation method is a time-consuming and energy-intensive process. Furthermore, a lower yield and a higher rate of product degradation are this method’s main drawbacks. Thus, the goal of this research is to optimize the extraction process parameters of cinnamon leaf essential oil in response to maximizing the yield while retaining quality by using response surface methodology (RSM). The application of extracted essential oil on minced beef to assess its preservative effect was also the other objective of this research. Extraction time (120–210 min), extraction temperature (105–115 ℃), and feed mass (300–600 g) were the chosen independent variables of the optimization experiment using central composite design (CCD). Furthermore, the extracted essential oil’s antibacterial and microbiological preservative activity on minced beef was evaluated. At extraction time of 175.43 min, extraction temperature of 105 °C, and a feed mass of 600 g, the optimum predicted value of cinnamon leaf essential oil yield and cinnamaldehyde concentration (% area) was 2.9% and 34.6%, respectively. Moreover, the second-order polynomial equation fits the experimental data for 20-run experimental data. The chemical composition of cinnamon leaf essential oil extracted at optimal conditions was dominated by eugenol (60.68%) and cinnamaldehyde (33.94%). Additionally, the optimally extracted cinnamon essential oil inhibited the growth of bacteria, particularly gram-positive bacteria. After twenty-one days of storage at 4 °C, total viable count of minced beef seasoned with cinnamon essential oil at concentration of 1.2% (v/v) was lower than 106 CFU/g. To conclude, optimized cinnamon leaf essential oil extraction process provides better yield while retaining its functional properties.

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来源期刊
Applied Biological Chemistry
Applied Biological Chemistry Chemistry-Organic Chemistry
CiteScore
5.40
自引率
6.20%
发文量
70
审稿时长
20 weeks
期刊介绍: Applied Biological Chemistry aims to promote the interchange and dissemination of scientific data among researchers in the field of agricultural and biological chemistry. The journal covers biochemistry and molecular biology, medical and biomaterial science, food science, and environmental science as applied to multidisciplinary agriculture.
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