从aśodhita和śodhita tāmra bhasma制备的Hridayarnava Rasa(草药矿物阿育吠陀配方)的急性口服毒性评价

C. Jagtap, M. Nariya, V. Shukla, P. Prajapati
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引用次数: 0

摘要

背景:在阿育吠陀中使用的草药矿物制剂如今被视为一个令人担忧的问题;尤其是含有汞、铅和铜等金属的。其中一种配方是hṛdayārṇava rasa (HR),它含有汞的黑色硫化物和tāmra巴斯玛。到目前为止,还没有这种配方的安全性资料。在本研究中,我们对aśodhita和śodhita形式的tāmra巴斯马制备的HR进行了急性口服毒性评估,以提供急性给药的安全性。材料与方法:śodhita tāmra bhasma制备的HR (STBHR)和aśodhita tāmra bhasma制备的HR (ATBHR)参照经典文献制备。急性毒性试验按照OECD 425准则进行评估,限量试验为2000 mg/kg。选取褐家鼠Wistar系白化雌性大鼠15只,随机分为3组,每组5只。一组作为对照组,另外两组作为实验组。将试验配方口服禁食过夜的雌性大鼠,记录14天的详细行为变化和死亡率。评估体重、血液学和生化指标以及一些重要器官的组织病理学研究等参数。结果:所有动物的行为、死亡率、体重和血液学参数均无明显变化。两种药物对血尿素、碱性磷酸酶、血清谷草酰转氨酶、血清谷丙转氨酶等生化指标均有影响。组织病理学检查,肝、肾切片出现退行性改变;而在ATBHR中相对较多。结论:两种试验药物的LD50均大于2000 mg/kg。结果表明,两种试验药物在较高剂量下均可引起肝肾毒性。然而,需要进一步的慢性毒性评价来确定长期给药的安全性。
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Acute oral toxicity evaluation of Hridayarnava Rasa (A Herbo-Mineral Ayurvedic Formulation) prepared from aśodhita and śodhita tāmra bhasma
Background: Herbo-mineral preparations used in Ayurveda are seen as a matter of concern nowadays; especially for containing metals such as mercury, lead, and copper. One of such formulations is hṛdayārṇava rasa (HR) which contains black sulfide of mercury and tāmra bhasma. Till date, no safety profile of this formulation is available. In the present study, acute oral toxicity of HR prepared from tāmra bhasma in aśodhita and śodhita form was evaluated to provide the safety profile on acute administration. Materials and Methods: HR prepared from śodhita tāmra bhasma (STBHR) and HR prepared from aśodhita tāmra bhasma (ATBHR) was prepared as per the classical reference. Acute toxicity test was evaluated as per OECD 425 guidelines with 2000 mg/kg as a limit test. Fifteen Wistar strain albino female rats (Rattus norvegicus) were randomly divided into three groups of five animals each. One group served as control, and other two served as experimental groups. Test formulation was administered orally to overnight fasted female rats, and detailed behavioral changes and mortality were recorded for 14 days. Parameters such as body weight, hematological and biochemical parameters, and histopathological study of some important organs were assessed. Results: No significant changes in behavior, mortality, body weight, and hematological parameters were observed in all the animals. Some biochemical parameters such as blood urea, alkaline phosphatase, serum glutamic oxaloacetic transaminase, and serum glutamic pyruvic transaminase were affected in both the test drugs. In histopathological study, sections of liver and kidney showed some degenerative changes; comparatively more in ATBHR. Conclusion: The results of the study demonstrate that LD50 of both the test drugs are higher than 2000 mg/kg. It was concluded that both the test drugs at higher dose levels can cause hepatorenal toxicity. However, further chronic toxicity evaluation is necessary to establish the safety profile on chronic administration.
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