M. K. Azameti, Pankaj Singh, K. Gaikwad, M. Dalal, A. Arora, V. Rai, J. Padaria
{"title":"热胁迫下小麦(Triticum aestivum L.)基因型差异表达新基因TaSSRP的分离与鉴定","authors":"M. K. Azameti, Pankaj Singh, K. Gaikwad, M. Dalal, A. Arora, V. Rai, J. Padaria","doi":"10.31742/ijgpb.82.2.12","DOIUrl":null,"url":null,"abstract":"Analysis of heat stress-responsive transcriptome data in wheat genotype Raj 3765 revealed that expression of salt stress root protein (SSRP) RS1 was observed to increase by 8.29 folds in the flag leaf at the grain filling stage. Further validation of the gene under heat stress condition (42oC for 6 hours) by qRT-PCR revealed an increase of 8.42 folds. In-silico studies revealed that the gene is localized mainly in the cytoplasm, and did not contain transmembrane helix. The presence of 15 phosphorylation sites as indicated by in-silico analysis could play significant roles in the heat stress tolerance.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Isolation and characterization of novel gene TaSSRP differentially expressed in wheat (Triticum aestivum L.) genotypes under heat stress\",\"authors\":\"M. K. Azameti, Pankaj Singh, K. Gaikwad, M. Dalal, A. Arora, V. Rai, J. Padaria\",\"doi\":\"10.31742/ijgpb.82.2.12\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Analysis of heat stress-responsive transcriptome data in wheat genotype Raj 3765 revealed that expression of salt stress root protein (SSRP) RS1 was observed to increase by 8.29 folds in the flag leaf at the grain filling stage. Further validation of the gene under heat stress condition (42oC for 6 hours) by qRT-PCR revealed an increase of 8.42 folds. In-silico studies revealed that the gene is localized mainly in the cytoplasm, and did not contain transmembrane helix. The presence of 15 phosphorylation sites as indicated by in-silico analysis could play significant roles in the heat stress tolerance.\",\"PeriodicalId\":13321,\"journal\":{\"name\":\"Indian Journal of Genetics and Plant Breeding\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2022-04-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Indian Journal of Genetics and Plant Breeding\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.31742/ijgpb.82.2.12\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Genetics and Plant Breeding","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.31742/ijgpb.82.2.12","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Isolation and characterization of novel gene TaSSRP differentially expressed in wheat (Triticum aestivum L.) genotypes under heat stress
Analysis of heat stress-responsive transcriptome data in wheat genotype Raj 3765 revealed that expression of salt stress root protein (SSRP) RS1 was observed to increase by 8.29 folds in the flag leaf at the grain filling stage. Further validation of the gene under heat stress condition (42oC for 6 hours) by qRT-PCR revealed an increase of 8.42 folds. In-silico studies revealed that the gene is localized mainly in the cytoplasm, and did not contain transmembrane helix. The presence of 15 phosphorylation sites as indicated by in-silico analysis could play significant roles in the heat stress tolerance.
期刊介绍:
Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.