Xiao Han, Maria Levkovets, Dmitry Lesovoy, Renhua Sun, Johan Wallerstein, Tatyana Sandalova, Tatiana Agback, Adnane Achour, Peter Agback, Vladislav Yu. Orekhov
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引用次数: 2
摘要
粘膜相关淋巴组织蛋白1 (MALT1)通过调节控制T细胞和B细胞发育和增殖的特异性细胞内信号通路,在适应性免疫应答中发挥关键作用。这些途径的功能障碍与高度侵袭性淋巴瘤的进展以及一系列不同免疫疾病的潜在发展有关。与其他信号介质不同的是,MALT1不仅通过与CARMA1和Bcl10蛋白形成CBM复合物而被激活,而且还作为蛋白酶裂解多种底物,通过NF-κB信号通路促进淋巴细胞增殖和存活。在此,我们提出了部分1H, 13C Ile/Val/ leu -甲基共振分配的人MALT1的paracaspase-IgL3结构域的单体载子形式。我们的研究结果为未来阐明MALT1的三维结构和动力学提供了坚实的基础,这是充分开发抑制剂的关键,并对其功能进行彻底的分子理解。
Assignment of IVL-Methyl side chain of the ligand-free monomeric human MALT1 paracaspase-IgL3 domain in solution
Mucosa-associated lymphoid tissue protein 1 (MALT1) plays a key role in adaptive immune responses by modulating specific intracellular signalling pathways that control the development and proliferation of both T and B cells. Dysfunction of these pathways is coupled to the progress of highly aggressive lymphoma as well as to potential development of an array of different immune disorders. In contrast to other signalling mediators, MALT1 is not only activated through the formation of the CBM complex together with the proteins CARMA1 and Bcl10, but also by acting as a protease that cleaves multiple substrates to promote lymphocyte proliferation and survival via the NF-κB signalling pathway. Herein, we present the partial 1H, 13C Ile/Val/Leu-Methyl resonance assignment of the monomeric apo form of the paracaspase-IgL3 domain of human MALT1. Our results provide a solid ground for future elucidation of both the three-dimensional structure and the dynamics of MALT1, key for adequate development of inhibitors, and a thorough molecular understanding of its function(s).
期刊介绍:
Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties.
Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.