Diana Almeida , Daniela Machado , Sérgio Sousa , Catarina Leal Seabra , Joana Cristina Barbosa , José Carlos Andrade , Ana Maria Gomes , Ana Cristina Freitas
{"title":"乳化/内凝胶微胶囊化对嗜粘阿克曼氏菌长时间储存和模拟胃肠道生存能力的影响","authors":"Diana Almeida , Daniela Machado , Sérgio Sousa , Catarina Leal Seabra , Joana Cristina Barbosa , José Carlos Andrade , Ana Maria Gomes , Ana Cristina Freitas","doi":"10.1016/j.fhfh.2022.100084","DOIUrl":null,"url":null,"abstract":"<div><p><em>Akkermansia muciniphila</em> is a common human intestinal commensal with a mucin-degrading nature. Its immunomodulatory characteristics and regulatory role of mucus layer and gut barrier integrity highlight the potential benefits of using this bacterium as an interventional player against inflammatory/cardio-metabolic disorders. In this work, we evaluate the effect of microencapsulation by the emulsification/internal gelation method on <em>A. muciniphila</em> survival during aerobic storage (0, 15, 30 and 95 days) and subsequent exposure to simulated gastrointestinal passage, in comparison with that of free cells. The present results show that microencapsulation by internal gelation promotes a 64.4 % entrapment efficacy of <em>A. muciniphila</em> cells (maintaining a 10<sup>8</sup> order of magnitude for cell viability). Moreover, physical characterization showed that microparticles mean size was 53,5 ± 12,1 µm and, as observed by electron scanning microscopy, microcapsules were spherical in shape. More importantly, as storage time increased, encapsulated <em>A. muciniphila</em> demonstrated higher stability in GI conditions, when compared to its free counterpart. In conclusion, microencapsulation by internal gelation seems to be an appropriate strategy in protecting <em>A. muciniphila</em> against the detrimental gastrointestinal transit after long periods of aerobic refrigerated storage.</p></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667025922000310/pdfft?md5=67083384d7b2256ca0bd9791f0d42795&pid=1-s2.0-S2667025922000310-main.pdf","citationCount":"3","resultStr":"{\"title\":\"Effect of emulsification/internal gelation-based microencapsulation on the viability of Akkermansia muciniphila upon prolonged storage and simulated gastrointestinal passage\",\"authors\":\"Diana Almeida , Daniela Machado , Sérgio Sousa , Catarina Leal Seabra , Joana Cristina Barbosa , José Carlos Andrade , Ana Maria Gomes , Ana Cristina Freitas\",\"doi\":\"10.1016/j.fhfh.2022.100084\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Akkermansia muciniphila</em> is a common human intestinal commensal with a mucin-degrading nature. Its immunomodulatory characteristics and regulatory role of mucus layer and gut barrier integrity highlight the potential benefits of using this bacterium as an interventional player against inflammatory/cardio-metabolic disorders. In this work, we evaluate the effect of microencapsulation by the emulsification/internal gelation method on <em>A. muciniphila</em> survival during aerobic storage (0, 15, 30 and 95 days) and subsequent exposure to simulated gastrointestinal passage, in comparison with that of free cells. The present results show that microencapsulation by internal gelation promotes a 64.4 % entrapment efficacy of <em>A. muciniphila</em> cells (maintaining a 10<sup>8</sup> order of magnitude for cell viability). Moreover, physical characterization showed that microparticles mean size was 53,5 ± 12,1 µm and, as observed by electron scanning microscopy, microcapsules were spherical in shape. More importantly, as storage time increased, encapsulated <em>A. muciniphila</em> demonstrated higher stability in GI conditions, when compared to its free counterpart. In conclusion, microencapsulation by internal gelation seems to be an appropriate strategy in protecting <em>A. muciniphila</em> against the detrimental gastrointestinal transit after long periods of aerobic refrigerated storage.</p></div>\",\"PeriodicalId\":12385,\"journal\":{\"name\":\"Food Hydrocolloids for Health\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2022-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2667025922000310/pdfft?md5=67083384d7b2256ca0bd9791f0d42795&pid=1-s2.0-S2667025922000310-main.pdf\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food Hydrocolloids for Health\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2667025922000310\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Hydrocolloids for Health","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667025922000310","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
Effect of emulsification/internal gelation-based microencapsulation on the viability of Akkermansia muciniphila upon prolonged storage and simulated gastrointestinal passage
Akkermansia muciniphila is a common human intestinal commensal with a mucin-degrading nature. Its immunomodulatory characteristics and regulatory role of mucus layer and gut barrier integrity highlight the potential benefits of using this bacterium as an interventional player against inflammatory/cardio-metabolic disorders. In this work, we evaluate the effect of microencapsulation by the emulsification/internal gelation method on A. muciniphila survival during aerobic storage (0, 15, 30 and 95 days) and subsequent exposure to simulated gastrointestinal passage, in comparison with that of free cells. The present results show that microencapsulation by internal gelation promotes a 64.4 % entrapment efficacy of A. muciniphila cells (maintaining a 108 order of magnitude for cell viability). Moreover, physical characterization showed that microparticles mean size was 53,5 ± 12,1 µm and, as observed by electron scanning microscopy, microcapsules were spherical in shape. More importantly, as storage time increased, encapsulated A. muciniphila demonstrated higher stability in GI conditions, when compared to its free counterpart. In conclusion, microencapsulation by internal gelation seems to be an appropriate strategy in protecting A. muciniphila against the detrimental gastrointestinal transit after long periods of aerobic refrigerated storage.