乳化/内凝胶微胶囊化对嗜粘阿克曼氏菌长时间储存和模拟胃肠道生存能力的影响

IF 4.6 Q1 CHEMISTRY, APPLIED Food Hydrocolloids for Health Pub Date : 2022-12-01 DOI:10.1016/j.fhfh.2022.100084
Diana Almeida , Daniela Machado , Sérgio Sousa , Catarina Leal Seabra , Joana Cristina Barbosa , José Carlos Andrade , Ana Maria Gomes , Ana Cristina Freitas
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引用次数: 3

摘要

嗜粘菌是一种常见的人类肠道共生菌,具有降解粘蛋白的性质。它的免疫调节特性和黏液层和肠道屏障完整性的调节作用突出了使用这种细菌作为炎症/心脏代谢紊乱的介入参与者的潜在益处。在这项工作中,我们评估了乳化/内凝胶法微胶囊化对嗜粘杆菌在有氧储存(0、15、30和95天)和随后暴露于模拟胃肠道中的存活的影响,并与自由细胞进行了比较。本研究结果表明,内部凝胶微胶囊化可使嗜粘杆菌细胞的包封效率提高64.4%(维持细胞活力108个数量级)。物理表征表明,微胶囊的平均粒径为53,5±12,1µm,电子扫描显微镜观察到微胶囊呈球形。更重要的是,随着储存时间的增加,包封的嗜粘杆菌在胃肠道条件下表现出更高的稳定性。总之,内部凝胶微胶囊化似乎是保护嗜粘杆菌在长时间有氧冷藏后免受有害胃肠道运输的一种适当策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Effect of emulsification/internal gelation-based microencapsulation on the viability of Akkermansia muciniphila upon prolonged storage and simulated gastrointestinal passage

Akkermansia muciniphila is a common human intestinal commensal with a mucin-degrading nature. Its immunomodulatory characteristics and regulatory role of mucus layer and gut barrier integrity highlight the potential benefits of using this bacterium as an interventional player against inflammatory/cardio-metabolic disorders. In this work, we evaluate the effect of microencapsulation by the emulsification/internal gelation method on A. muciniphila survival during aerobic storage (0, 15, 30 and 95 days) and subsequent exposure to simulated gastrointestinal passage, in comparison with that of free cells. The present results show that microencapsulation by internal gelation promotes a 64.4 % entrapment efficacy of A. muciniphila cells (maintaining a 108 order of magnitude for cell viability). Moreover, physical characterization showed that microparticles mean size was 53,5 ± 12,1 µm and, as observed by electron scanning microscopy, microcapsules were spherical in shape. More importantly, as storage time increased, encapsulated A. muciniphila demonstrated higher stability in GI conditions, when compared to its free counterpart. In conclusion, microencapsulation by internal gelation seems to be an appropriate strategy in protecting A. muciniphila against the detrimental gastrointestinal transit after long periods of aerobic refrigerated storage.

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CiteScore
4.50
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审稿时长
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