用直接紫外检测法快速简便高效液相色谱法定量药物剂型中磷霉素-卓元醇的新方法的概念

IF 2.3 Q3 PHARMACOLOGY & PHARMACY Scientia Pharmaceutica Pub Date : 2022-05-24 DOI:10.3390/scipharm90020035
M. Piponski, Tanja Bakovska Stoimenova, T. Melnyk, S. Kovalenko, Elena Lazarevska Todevska, Marjan Velkovski, S. Deeb, Yuriy Mysula, L. Logoyda
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引用次数: 1

摘要

在没有对分析物进行任何衍生和修饰的情况下,开发了两种不同的概念来开发直接HPLC-UV方法来定量磷霉素-氨丁醇。在第一个概念中,在流动相中不使用烷基胺作为离子对的情况下,通过使用氰基丙基CN和强阴离子交换柱,我们研究了它们的高极性和阴离子交换力的可能性以及在没有额外试剂或改性剂的帮助下保留、分离和检测氨丁醇的机制。在第二个概念中,具有不同特性和供应商的最常见的反相C18柱与链中具有3-10个C原子的不同长度的烷基胺组合进行了测试。在我们的研究中,我们发现磷霉素与6–10个碳原子烷基长度的脂族链的离子配对表现出烷基配对的丁三醇分子与十八烷基硅烷或C18键合的RP柱之间最合适的相互作用强度,以在色谱图上实现最佳的保留率、选择性和峰形,并有可能微调洗脱时间。我们方法概念的简单性省略了对昂贵而复杂的柱的需求,如HILIC、C30石墨碳和基于混合模式的柱,以更容易保留、分离和测定磷霉素,并实现其定量目的,特别是在常规质量控制实验室的高通量分析中。
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Concepts for New Rapid Simple HPLC Method for Quantification of Fosfomycin Trometamol in Pharmaceutical Dosage Forms with Direct UV Detection
Two different concepts for developing direct HPLC-UV methods for quantifying fosfomycin trometamol were developed without any derivatization and modification of the analyte. In the first concept, without the use of alkylamines as ion-pairs in the mobile phase, by using cyanopropyl CN and a strong anion-exchanger column, we investigated the possibility of their highly polar and anion-exchanging forces and mechanisms to retain, separate and detect trometamol without the help of additional agents or modifiers. In the second concept, the most frequent reversed-phase C18 columns with different characteristics and vendors were tested in combination with different length-based alkylamines with 3–10 C atoms in their chains. In our research, we found that the ion-pairing of fosfomycin with 6–10 C-atom-based alkyl-length of aliphatic chains manifested the most appropriate strength of interactions between alkyl-paired trometamol molecules and octadecylsilane or C18 bonded RP column to achieve optimal retention, selectivity and peak shape on chromatograms, with the possibility for the fine-tuning of elution time. The simplicity of our method concept omits the need for expensive and sophisticated columns like HILIC, C30 graphite carbon, and mixed-mode-based columns for easier retaining, separation, and determination of fosfomycin, and for its quantification purposes, especially in high-throughput analyses in regular quality-control laboratories.
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来源期刊
Scientia Pharmaceutica
Scientia Pharmaceutica Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
4.60
自引率
4.00%
发文量
67
审稿时长
10 weeks
期刊最新文献
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