绿色木霉深层培养生产能力的监测

O. Georgieva, N. Karadzhova
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引用次数: 0

摘要

摘要研究了营养培养基、接种量和初始酸度对拮抗真菌绿色三叶草生物量积累的影响。作为所进行的研究的结果,生物技术参数被优化用于通过浸没培养方法生产生物产品。已经开发出一种平衡的半合成营养培养基,在该培养基中,真菌进行完整的个体发生周期,形成厚垣孢子和扁孢子虫。所生产的在1ml中具有超过1.106厚垣孢子滴度的生物质既可用于制备液体形式的制剂,也可用于在固体基质上制备制剂。
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Monitoring the productivity of Trichoderma viride strain in submerged cultivation
Abstract. The influence of the nutrient medium, the amount of inoculate and the initial acidity on the accumulation of the biomass of the antagonistic fungus Trihoderma viride during submerged cultivation was studied. As a result of the conducted research, biotechnological parameters were optimized for the production of a biological product by the method of submerged cultivation. A balanced semi-synthetic nutrient medium has been developed in which the fungus performs a full cycle of ontogenesis, forming chlamydospores and phialoconidia. The produced biomass with a titer of more than 1.106 chlamydospores in 1 ml can be used both for the production of liquid form of the preparation and for the production of preparations on solid substrates.
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