布帕伐酮在水牛中同时感染伊文氏锥虫和环孢杆菌的pcr效果

IF 0.2 4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Buffalo Bulletin Pub Date : 2023-06-30 DOI:10.56825/bufbu.2023.4224141
V. Agrawal, G. Das, Amit Jaiswal, A. Jayraw, G. Jatav, M. Shakya, G. Goyal
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引用次数: 0

摘要

在Madhypradesh Mahakaushal地区的一个农场饲养了五头水牛,它们在2015年7月5至7岁,有体温升高、鼻腔分泌物、眼球突出、兴奋、流泪、流涎、呼吸困难、全身淋巴结病,尤其是肩胛前淋巴结。通过血液涂片和聚合酶链式反应分析,确定所有水牛都有埃文氏锥虫和环状锥虫的混合感染。所有五头水牛的治疗都是在隔日通过单次深肌注射加速地美那嗪3.5mg/kg体重以及三个剂量的长效土霉素20mg/kg体重开始的。在第四天(3例)单次注射布帕奎酮,并在48小时间隔(2例)注射两剂布帕奎(2.5 mg kg-1)。只有3头水牛在单次注射布帕奎酮后表现出平静的恢复。当再次通过PCR分析所有五只水牛的血液样本时,发现除了两只水牛(两例服用了两剂布帕奎酮)外,所有样本都对埃文西和环状曲霉菌呈阴性,尽管服用了第二剂布帕奎酮,但它们对环状曲霉菌仍呈阳性。该研究表明,环孢霉对布帕奎酮的药效或耐药性可能降低。
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Efficacy of buparvaquone in pcr confirmed concurrent infection of Trypansoma evansi and Theileria annulata in buffaloes
Five buffaloes, 5 to 7 years old in the month of July 2015 with history of high rise of temperature, nasal discharge, exophthalmia, excitement, lacrimation, salivation, dyspnea, generalised lymphadenopathy, especially in the prescapular lymph nodes were attended at a farm in Mahakaushal region of Madhypradesh. By blood smear and polymerase chain reaction analysis, it was determined that all the buffaloes had mixed infections of T. evansi and T. annulata. The treatment of all five buffaloes was initiated with single deep intramuscular injection of diminazine acceturate 3.5 mg/kg body weight along with three dosages of long acting oxytetracycline 20 mg/kg b.wt. on alternate day. A single injection of buparvaquone on the fourth day (in 3 cases) and two doses of buparvaquone (2.5 mg kg-1) at 48 h intervals (in 2 cases) were administered. Only 3 buffaloes showed uneventful recovery after a single injection with buparvaquone. When the blood samples of all the five buffaloes were again analyzed by PCR and found all the samples were negative for T. evansi and T. annulata except two buffaloes (Two cases hading two doses of buparvaquone) which were positive for T. annulata in spite of giving the second dose of  buparvaquone. The study indicated that there might be reduced efficacy or resistance of T. annulata against buparvaquone.
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Buffalo Bulletin
Buffalo Bulletin AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
0.50
自引率
0.00%
发文量
53
审稿时长
>12 weeks
期刊介绍: Buffalo Bulletin is published quarterly in January-March, April-June, July-September and October-December. Contributions on any aspect of research or development, progress reports of projects and news on buffalo will be considered for publication in the bulletin.
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