酸浆对肠道细菌和真菌微生态系统的调节作用

IF 4.7 4区 医学 Q1 CHEMISTRY, MEDICINAL Chinese Herbal Medicines Pub Date : 2023-10-01 DOI:10.1016/j.chmed.2023.02.003
Yanan Yang , Xiaohui Zhao , Yong Xie , Chongming Wu
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引用次数: 0

摘要

目的肠道微生物群是调节人体健康和药物疗效的复杂微生态系统。白Physalis alkekengi (PAL)是一种历史悠久的食用中药。PAL的几种药理作用已得到证实,其治疗作用与肠道细菌有关。然而,PAL对肠道细菌种类和肠道真菌的详细调节在很大程度上仍然未知。因此,我们设计了正常小鼠的初步实验,以揭示PAL对肠道细菌和真菌的调节作用,并探讨它们之间的相互作用。方法以正常C57BL/6小鼠为实验对象,口服PAL水提物4周。利用16S rRNA全长和ITS1/2基因测序分别检测PAL处理后肠道细菌和肠道真菌的分类群。结果口服PAL可显著增加Duncaniella spp和Kineothrix alysoides等抗炎细菌种类,而减少Mucispirillum schaedleri等促炎细菌种类。同时,PAL增加了肠道真菌赭曲霉、枝孢霉和Alternaria sp.的丰度,减少了紫青霉。相关网络分析发现两个共存的微生物群(组1和组2)彼此负相关。第1组以富含pal的细菌和真菌为主,第2组以富含正常食物的细菌和真菌为主。在1组中,单歧Antrodia、clavatus Aspergillus、kuhlmanii Mortierella、Sarcinomyces sp. MA 4787与globosum双歧杆菌、Romboutsia ilealis等呈显著正相关。在第2组中,毛藻(Chaetomium subspirilliferum)、兰花Septoria orchidearum和热带头孢(Cephaliophora tropica)与乳酸菌(Lactobacillus sp .)呈正相关。结论本初步研究首次证实了PAL对肠道细菌和肠道真菌的调节作用,为阐明PAL的药理作用机制提供了线索。
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Modulative effect of Physalis alkekengi on both gut bacterial and fungal micro-ecosystem

Objective

Gut microbiome is an intricate micro-ecosystem mediating the human health and drug efficacy. Physalis alkekengi (PAL) is an edible and time-honored traditional Chinese medicine. Several pharmacological effects of PAL have been verified and gut bacteria are implied in its therapeutic actions. However, the detailed modulation of PAL on gut bacterial species and on gut fungi remains largely unknown. We, therefore, designed a preliminary experiment in normal mice to reveal the modulation effect of PAL on both gut bacteria and fungi, and explore the interaction between them.

Methods

Herein, the aqueous extract of PAL was orally administrated to normal C57BL/6 mice for four weeks. The full-length 16S rRNA and ITS1/2 gene sequencing were explored to detect the taxa of gut bacteria and gut fungi after PAL treatment, respectively.

Results

Oral administration of PAL notably enriched anti-inflammatory bacterial species such as Duncaniella spp. and Kineothrix alysoides, whereas decreased pro-inflammatory species such as Mucispirillum schaedleri. Simultaneously, PAL increased the abundance of gut fungi Aspergillus ochraceus, Cladosporium sp. and Alternaria sp., and decreased Penicillium janthinellum. Correlation network analysis identified two co-existing microbial groups (groups 1 and 2) that were negatively associated with each other. The group 1 comprised PAL-enriched bacteria and fungi, while group 2 was mainly normal chow-enriched bacteria and fungi. In group 1, Antrodia monomitica, Aspergillus clavatus, Mortierella kuhlmanii and Sarcinomyces sp. MA 4787 were positively correlated with Bifidobacterium globosum, Romboutsia ilealis and so on. In group 2, Chaetomium subspirilliferum, Septoria orchidearum and Cephaliophora tropica were positively related to Lactobacillus spp.

Conclusion

Altogether, this preliminary study first demonstrated the modulation effect of PAL on both gut bacteria and gut fungi, which may shed light on the elucidation of PAL’s pharmacological mechanism.

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来源期刊
Chinese Herbal Medicines
Chinese Herbal Medicines CHEMISTRY, MEDICINAL-
CiteScore
4.40
自引率
5.30%
发文量
629
审稿时长
10 weeks
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