四种常见蔬菜的清除自由基活性、总还原力、总酚和黄酮含量

Doriane E. Djeussi, J. A. Noumedem Kenfack, Christelle Noumedem Anangmo, S. R. Simeni Njonnou, C. Ngongang Ouankou, I. B. Yossa Nzeuwa, C. Yamssi, François Zambou Ngoufack, J. Kuiaté, V. Kuete
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引用次数: 3

摘要

目的:本研究旨在评价四种蔬菜提取物的抗氧化活性,这四种蔬菜分别是:Beta vulgaris、Raphanus sativus和Brassica oleracea的两个品种(红色和绿色)。方法:采用2,2-二苯基-1-丙酰肼(DPPH)、氮氧化物(NO)、总还原力、总酚类和黄酮类化合物含量五种不同的方法对这些提取物的抗氧化性能进行评价。结果:四种蔬菜提取物对DPPH的自由基清除活性均在129.77~323.64µg/ml之间,对NO自由基的抑制活性在1454、52~4479、97µg/ml之间。四种蔬菜提取物还显示出在2.41和9.37AAE之间的总还原能力(毫克抗坏血酸当量/克干燥提取物)。这些抗氧化活性可以通过存在不同的抗氧化化合物来证明,如存在于所有研究的蔬菜提取物中的总酚含量,或黄酮类化合物,其也存在于所有植物中,总含量在0.1和0.25RE之间(芦丁当量/克干燥提取物)。结论:本研究中显示的不同抗氧化活性提供了科学证据,证明喀麦隆常见的一些蔬菜,包括马齿苋、马齿苋和寻常B.vulgaris,可以作为膳食补充剂或预防药物,用于管理氧化应激和相关病理。
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Radical Scavenging Activities, Total Reducing Power, Total Phenolic and Flavonoids Contents of Four Common Vegetables
Aim: This study aimed at evaluating the antioxidant activities of extracts from four vegetables namely: Beta vulgaris, Raphanus sativus, and two varieties (red and green) of Brassica oleracea. Method: The antioxidant properties of these extracts were assessed using five different methods including 2,2-diphényl 1-picrylhydrazyl (DPPH), Nitrogen Oxyde (NO), total reducing power, total phenolic and flavonoids content. Results: All the four vegetable extracts showed free radical-scavenging activity against DPPH· with RSa50 (Radical scavenging activity 50) ranging between 129.77 and 323.64 µg/ml, and inhibitory activity against NO radical (RSa50 ranging from 1454,52 to 4479,97 µg/ml). The four vegetable extracts also showed total reducing powers ranging between 2.41 and 9.37 AAE (mg ascorbic acid equivalents per gram of dried extract). These antioxidant activities can be justified by the presence of different antioxidant compounds like total phenol contents which were present in all studied vegetable extracts with quantities varying between 4.37 and 11.83 GAE (mg of garlic acid equivalents per gram of dried extract) of dry extract, or flavonoids which were also present in all the plants with total contents ranging between 0.1 and 0.25 RE (rutin equivalents per gram of dried extract). Conclusion: The different antioxidant activities demonstrated in this study provide scientific evidence that some vegetables commonly consumed in Cameroon including B. oleracea, R. sativus and B. vulgaris can serve as a dietary supplement or in preventive medicine in the management of oxidative stress and associated pathologies.  
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