厚叶Phakopsora pachyrhizi是哥伦比亚大豆锈病的主要病原体

Anibal Leonidas Tapiero-Ortiz, N. López-Cardona, A. Guevara-Castro, Sandra Milena Rodríguez-Triana, Edisson Chavarro-Mesa, J. Ángel-Diaz
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引用次数: 1

摘要

种植在哥伦比亚东部平原的大豆作物受到锈病的影响。这种疾病仍被哥伦比亚农业部(ICA)正式认定为美洲大豆锈病(由黑腹Phakopsora meibomiae引起),对大豆种植区造成严重损害。自2004年以来,在植物营养阶段的上半部分已观察到锈病的症状,如红褐色病变。在2005年和2018年,从实验区和商业大豆田收集了受感染的叶组织和脲孢子。一旦形态鉴定和Koch的假设证实了Phakopsora spp.的存在,就进行分子鉴定,以确定与该疾病相关的病原体到物种水平。这是基于rDNA的内部转录间隔区(ITS)和PCR扩增特异性引物的标准程序,用于黑腹Phakopsora meibomiae和厚根假单胞菌。将得到的序列与GenBank/NCBI数据库进行blast比对。考虑到2005年和2018年采集的样本,结果表明,P. pachyrhizi Sydow实际上是哥伦比亚大豆锈病的病原体。厚根菌ITS-rDNA序列保存于GenBank,登录号为MK933723 ~ MK933731。这一发现已报告给哥伦比亚国际检疫机构,以便他们能够正式更新哥伦比亚大豆病原菌的植物检疫状况。
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Phakopsora pachyrhizi is the major pathogen associated with soybean rust in Colombia
ABSTRACT Soybean crops grown in the plains of eastern Colombia have been affected by the incidence of rust. This disease, still officially regarded as American soybean rust (caused by Phakopsora meibomiae) by the Colombian Department of Agriculture (ICA), causes serious damage to soybean growing areas. Symptoms of rust, such as reddish-brown lesions, have been observed since 2004 in the upper half of plants during vegetative stages. In 2005 and 2018, infected leaf tissues and uredinospores were collected from an experimental area and from commercial soybean fields. Once morphological identification and Koch’s postulates confirmed the presence of Phakopsora spp., molecular characterization was performed to identify the pathogen associated with the disease to species level. This was based on standard procedures using internal transcribed spacer (ITS) regions of rDNA and PCR amplification with specific primers for Phakopsora meibomiae and P. pachyrhizi. The obtained sequences were BLASTed against GenBank/NCBI data bank. Results indicated that P. pachyrhizi Sydow is in fact the causative agent of soybean rust in Colombia, considering the samples collected in 2005 and 2018. The ITS-rDNA sequences of P. pachyrhizi were deposited at GenBank under the accession numbers MK933723 to MK933731. This finding was reported to ICA, so that they could officially update the phytosanitary status of this soybean pathogen in Colombia.
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来源期刊
Summa Phytopathologica
Summa Phytopathologica Agricultural and Biological Sciences-Plant Science
CiteScore
0.90
自引率
0.00%
发文量
13
期刊介绍: The Summa Phytopathologica is a publication of the São Paulo State Plant Pathology Association (APF), Botucatu SP. Summa Phytopathologica (SP) is dedicated to publishing technical and scientific articles that describe original research in the area of Plant Pathology that may contribute significantly to its progress. SP accepts papers written in Portuguese, English, or Spanish. Its abbreviated title, Summa Phytopathol., should be used in bibliographies, footnotes and bibliographical references and strips.
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