PEMERICAN乙型肝炎表面抗原(HBsAg)和抗HBs PADA IBU HAMIL

Herlando Sinaga, I. Latif, Nasir Pangulu
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Serum tersebut digunakan dalam pemeriksaan HBsAg dengan metode Immunokromatografi sedangkan Pemeriksaan HBsAg didasarkan pada prinsip Double Antibody Sandwich Immunoassay untuk penentuan HBsAg. Pengambilan sempel  dilaksanakan selama 1 bulan, Penelitian ini dilakukan dengan menggunakan Rapid Test HBsAg merk SD Bioline dengan sensitivity >99%, specificity >99% dan strip test Anti-HBs merk Answer dengan sensitivity >99% dan specificity >99,5%. Penelitian ini menggunakan metode analisis deskriptif untuk mengetahui hasil pemeriksaan HBsAg dan Anti-HBs pada ibu hamil. Pemeriksaan HBsAg dan Anti-HBs didapatkan hasil untuk HBsAg sebanyak 13% positif terinfeksi Hepatitis B dan sebanyak 87% lainnya diperoleh hasil negatif, dan hasil pemeriksaan Anti-HBs didapatkan hasil sebanyak 15% memiliki antibodi Hepatitis B dan sebanyak 85% lainnya diperoleh hasil negatif. Diisimpulkan bahwa dari pemeriksaan HBsAg dan Anti-HBs masih ditemukan sempel positif Hepatitis B. 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Sampling was carried out for 1 month, this research  was carried out using HBsAg Rapid Test brand SD Bioline with sensitivity >99% specificity >99%  and Anti-HBs test strip brand Answer with sensitivity >99% specificity >99.5%. This research  used a descriptive analysis method to determine the results of HBsAg and Anti-HBs examination in pregnant women. HBsAg and Anti-HBs examination were obtained for 13% HBsAg positive infected with Hepatitis B and as many as 87% obtained negative results, and the results of Anti-HBs examination showed that 15% had Hepatitis B antibodies and 85% obtained negative results. 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引用次数: 3

摘要

摘要孕妇孕前HBsAg检测是对乙型肝炎垂直传播的筛查。本研究旨在了解城市艺术中心和Marthen Indey Jayapura医院对孕妇进行HBsAg和抗-HBs检查的结果。本研究中孕妇的血清样本数量为60份;30份来自城市艺术中心的样本和30份来自Marthen Indey医院的样本。所使用的HBsAg和抗-HBs检测方法是免疫层析方法。它从孕妇身上的Vena血液开始,然后通过离心机产生血清。血清用于使用免疫色谱法的HBsAg检测,而HBsAg的检测基于双抗体夹心免疫测定原理来测定HBsAg。本研究使用灵敏度>99%、特异性>99%的快速检测HBsAg SD Bioline品牌和灵敏度>99%和特异性>99.5%的条形检测抗-HBs品牌Answer进行1个月的简单摄取[UNK]。本研究采用描述性分析方法来确定孕妇的HBsAg和抗-HBs检查结果。HBsAg和抗-HBs检测结果显示,HBsAg阳性的乙型肝炎感染率高达13%,其他阴性的结果高达87%,抗-HBss检测结果显示有15%的乙型肝炎抗体,其他阴性结果高达85%。结论HBsAg和抗-HBs对乙型肝炎仍呈阳性。本研究旨在确定Sentani Kota健康中心和Marthen Indey医院孕妇的HBsAg和抗-HBs检查结果。本研究孕妇血清样本数量为60份;Sentani Kota健康中心的30份样本和Marthen Indies医院的30份样品。所用的HBsAg和抗-HBs检查方法是免疫色谱法。从孕妇采集静脉血开始,比用离心机生产血清更容易。血清用于HBsAg检测采用免疫色谱法,而HBsAg检查则基于双抗体夹心免疫测定原理进行HBsAg测定。采样1个月,本研究采用HBsAg快速检测品牌SD Bioline,灵敏度>99%,特异性>99%,抗-HBs试纸条品牌Answer,灵敏度>99%,特异性>99.5%。HBsAg和抗-HBs检测结果显示,13%的HBsAg阳性者感染了乙型肝炎,多达87%的人获得了阴性结果,抗-HBss检测结果显示15%的人具有乙型肝炎抗体,85%的人获得阴性结果。结论HBsAg和抗-HBs检查仍发现乙型肝炎阳性。
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PEMERIKSAAN HEPATITIS B SURFACE ANTIGEN (HBsAg) DAN Anti-HBs PADA IBU HAMIL
Abstrak Pemeriksaan HBsAg pada ibu hamil sebelum melakukan persalinan merupakan skrining adanya penularan Hepatitis B secara vertikal. Penelitian ini bertujuan untuk mengetahui hasil pemeriksaan HBsAg dan Anti-HBs pada ibu hamil di Puskesmas Sentani Kota dan Rumah Sakit Marthen Indey Jayapura. Jumlah sampel serum ibu hamil dalam penelitian ini adalah 60 sampel; 30 sampel dari Puskesmas Sentani Kota dan 30 Sampel Rumah Sakit Marthen Indey. Metode pemeriksaan HBsAg dan Anti-HBs yang digunakan adalah metode Immunokromatografi. Diawali dengan pengambilan darah Vena pada ibu hamil kemudian melakukan sentrifuge untuk menghasilkan serum. Serum tersebut digunakan dalam pemeriksaan HBsAg dengan metode Immunokromatografi sedangkan Pemeriksaan HBsAg didasarkan pada prinsip Double Antibody Sandwich Immunoassay untuk penentuan HBsAg. Pengambilan sempel  dilaksanakan selama 1 bulan, Penelitian ini dilakukan dengan menggunakan Rapid Test HBsAg merk SD Bioline dengan sensitivity >99%, specificity >99% dan strip test Anti-HBs merk Answer dengan sensitivity >99% dan specificity >99,5%. Penelitian ini menggunakan metode analisis deskriptif untuk mengetahui hasil pemeriksaan HBsAg dan Anti-HBs pada ibu hamil. Pemeriksaan HBsAg dan Anti-HBs didapatkan hasil untuk HBsAg sebanyak 13% positif terinfeksi Hepatitis B dan sebanyak 87% lainnya diperoleh hasil negatif, dan hasil pemeriksaan Anti-HBs didapatkan hasil sebanyak 15% memiliki antibodi Hepatitis B dan sebanyak 85% lainnya diperoleh hasil negatif. Diisimpulkan bahwa dari pemeriksaan HBsAg dan Anti-HBs masih ditemukan sempel positif Hepatitis B. Abstract [ EXAMINATION OF HIGATITIS ANTIGEN SURFACE (HBsAg) AND Anti-HBs IN PREGNANT MOMS AS A HEPATITIS B BREEDING SCREENING ] HBsAg examination in pregnant women before labour is a screening for vertical hepatitis B transmission. This research aims to determine the results of HBsAg and Anti-HBs examination in pregnant women at Sentani Kota Health Center and Marthen Indey Hospital. The number of serum samples of pregnant women in this research was 60 samples; 30 samples from Sentani Kota Health Center and 30 Samples of Marthen Indies Hospital. The method of HBsAg and Anti-HBs examination used is the Immunochromatography method. Beginning with taking venous blood in pregnant women than doing a centrifuge to produce serum. The serum is used in HBsAg examination with the Immunochromatography method while HBsAg examination is based on the principle of Double Antibody Sandwich Immunoassay for HBsAg determination. Sampling was carried out for 1 month, this research  was carried out using HBsAg Rapid Test brand SD Bioline with sensitivity >99% specificity >99%  and Anti-HBs test strip brand Answer with sensitivity >99% specificity >99.5%. This research  used a descriptive analysis method to determine the results of HBsAg and Anti-HBs examination in pregnant women. HBsAg and Anti-HBs examination were obtained for 13% HBsAg positive infected with Hepatitis B and as many as 87% obtained negative results, and the results of Anti-HBs examination showed that 15% had Hepatitis B antibodies and 85% obtained negative results. It was concluded that HBsAg and Anti-HBs examination still found positive for hepatitis B.
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