花生AhDGAT3基因的过表达增加了大豆的含油量

IF 3.3 2区 农林科学 Q1 AGRONOMY Agronomy-Basel Pub Date : 2023-09-07 DOI:10.3390/agronomy13092333
Yang Xu, Fan Yan, Zhengwei Liang, Ying Wang, Jingwen Li, Lei Zhao, Xuguang Yang, Qingyu Wang, Jingya Liu
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引用次数: 0

摘要

大豆(Glycine max)是为人类提供植物油的主要油料作物。其育种研究的主要目的是提高总含油量。在肯尼迪途径中,二酰甘油酰基转移酶(DGAT)是一种将二酰甘油(DAG)转化为三酰甘油(TAG)的限速酶。在这里,AhDGAT3基因从花生中克隆并在野生型(WT)拟南芥中过表达。T3AhDGAT3转基因拟南芥种子中的总脂肪酸含量平均是WT的1.1倍。因此,将AhDGAT3。还通过基因组重新测序获得了特异性T-DNA插入区位置信息。高效气相色谱分析结果表明,转基因大豆植株的油酸(18:1)组成和总脂肪酸含量均显著高于WT,而亚油酸(18:2)含量远低于WT。农艺性状调查表明,AhDGAT3转基因大豆的数量性状和产量性状均优于WT。这些结果表明,转基因大豆中的脂肪酸,特别是油酸和总脂肪酸,因其过表达而增强。
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Overexpression of the Peanut AhDGAT3 Gene Increases the Oil Content in Soybean
Soybean (Glycine max) is the main oilseed crop that provides vegetable oil for human nutrition. The main objective of its breeding research is to increase the total oil content. In the Kennedy pathway, Diacylglycerol acyltransferase (DGAT) is a rate-limiting enzyme that converts diacylglycerol (DAG) to triacylglycerol (TAG). Here, the AhDGAT3 gene was cloned from peanut and overexpressed in the wild-type (WT) Arabidopsis. The total fatty acid content in T3AhDGAT3 transgenic Arabidopsis seeds was 1.1 times higher on average than that of the WT. Therefore, AhDGAT3 was transferred into the WT (JACK), and four T3 transgenic soybean lines were obtained, which proved to be positive using molecular biological detection. Specific T-DNA insertion region location information was also obtained via genome re-sequencing. The results of high-performance gas chromatography showed that the contents of oleic acid (18:1) composition and total fatty acids in transgenic soybean plants were significantly higher than that of the WT. However, linoleic acid (18:2) was much lower compared to the WT. The agronomic trait survey showed that the quantitative and yield traits of AhDGAT3 transgenic soybean were better than those of the WT. These results suggest that fatty acids in transgenic soybeans, especially oleic acid and total fatty acid, are enhanced by the over-expression of AhDGAT3.
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来源期刊
Agronomy-Basel
Agronomy-Basel Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
6.20
自引率
13.50%
发文量
2665
审稿时长
20.32 days
期刊介绍: Agronomy (ISSN 2073-4395) is an international and cross-disciplinary scholarly journal on agronomy and agroecology. It publishes reviews, regular research papers, communications and short notes, and there is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.
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