牙科x线摄影方式对儿童口腔黏膜细胞的基因毒性和细胞毒性作用

IF 0.3 Q4 PEDIATRICS Journal of Child Science Pub Date : 2023-01-01 DOI:10.1055/s-0043-1772206
B. Salman, Sona Rafieyan, Kasra Rahimipour, Narges Bayat
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引用次数: 0

摘要

摘要牙科射线照相术是检测和评估龋齿程度以及制定准确治疗计划的重要诊断工具。X射线照射没有安全限制。与成人相比,儿童暴露于X射线的相关风险更高,因为他们的细胞增殖率更高。本研究旨在评估牙科放射学方法对儿童口腔粘膜细胞的遗传毒性和细胞毒性影响。这项介入性研究评估了80名年龄在3至12岁之间的儿童,他们需要根尖周、全景、咬合或咬合加全景放射照相术来制定治疗计划。20名符合条件的患者被分配到上述四组中的每一组。口腔完全冲洗后,用塑料抹刀刮双侧颊粘膜细胞。将收集的样品直接安装在显微镜载玻片上,空气干燥后,用80%甲醇和Giemsa染色固定。然后在400倍放大的光学显微镜下检查细胞的细胞遗传学变化。数据用SPSS版本20制成表格并进行分析 < 0.001的显著性水平。结果显示,在所有四组中,放射线照相术后,核溶解、核破裂和固缩的频率都显著增加(p < 0.001)。此外,全景加咬合射线照相术后微核数量显著增加(p < 0.05)。全景、根尖周、咬合和咬合加全景射线照相的X射线照射可能具有细胞毒性,而咬合加全景放射照相对儿童也可能具有遗传毒性。
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Genotoxic and Cytotoxic Effects of Dental Radiographic Modalities on Buccal Mucosal Cells in Children
Abstract Dental radiography is an important diagnostic tool for the detection and assessment of the extent of dental caries and accurate treatment planning. There is no safe limit for X-ray exposure. The associated risks of X-ray exposure are higher in children due to a higher rate of cell proliferation in them, compared with adults. This study aimed to assess the genotoxic and cytotoxic effects of dental radiographic modalities on buccal mucosal cells in children. This interventional study evaluated 80 children between 3 and 12 years who required periapical, panoramic, bitewing, or bitewing plus panoramic radiography for treatment planning. Twenty eligible patients were assigned to each of the aforementioned four groups. Buccal mucosal cells were scraped bilaterally by a plastic spatula after complete rinsing of the oral cavity. The collected specimens were directly mounted on microscopic slides and after air-drying, they were fixed with 80% methanol and Giemsa stain. The cells were then inspected under a light microscope at 400x magnification for cytogenetic changes. Data were tabulated and analyzed by SPSS version 20 at a p  < 0.001 level of significance. The results showed a significant increase in the frequency of karyolysis, karyorrhexis, and pyknosis in all four groups after dental radiography ( p  < 0.001). Also, the number of micronuclei significantly increased after panoramic plus bitewing radiography ( p  < 0.05). X-ray exposure in panoramic, periapical, bitewing, and bitewing plus panoramic radiographies can be cytotoxic, while bitewing plus panoramic radiography can be genotoxic in children as well.
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