基于减毒疫苗的交叉保护新策略:以RNA病毒为载体控制DNA病毒

IF 3.3 2区 农林科学 Q1 AGRONOMY Agronomy-Basel Pub Date : 2023-09-07 DOI:10.3390/agronomy13092334
Mingjing Zhu, Shanshan Liu, Zhao Wang, Chengming Yu, Xuefeng Yuan
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引用次数: 0

摘要

植物病毒可以感染各种类型的植物,包括粮食和油料作物以及观赏花卉,威胁农业生产和粮食供应。交叉保护是对抗严重病毒株的有效策略。由于感染机制不同,涉及RNA病毒和DNA病毒的交叉保护病例往往依赖于使用相应的减毒株进行控制。在本研究中,我们利用RNA病毒组成员黄瓜花叶病毒(CMV)作为开发减毒疫苗的基础框架。我们通过插入番茄黄卷叶病毒(TYLCV) DNA病毒的相关序列,开发了四种疫苗。所有疫苗均显示可有效预防TYLCV感染,相对控制效率超过80%。随后,我们评估了这些疫苗对巨细胞病毒和TYLCV混合感染的预防作用。我们的研究结果表明,CMV (R2-2bPTI-TYC1C4)、CMV (R2-2bPTI-TYC1C4)和CMV (r2 - 2bptiiii - tyrep)在预防混合感染方面表现出显著的疗效。预接种后,番茄植株的病害指数由100降至56。本研究为通过交叉保护控制TYLCV提供了理论基础和物质资源,同时为利用弱RNA病毒疫苗控制DNA病毒提供了可行的策略。
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A New Strategy of Cross-Protection Based on Attenuated Vaccines: RNA Viruses Are Used as Vectors to Control DNA Viruses
Plant viruses can infect various types of plants, including food and oil crops, and ornamental flowers, threatening agricultural production and food supply. Cross-protection is an efficient strategy against severe viral strains. Due to distinct infection mechanisms, cross-protection cases involving RNA viruses and DNA viruses often rely on the utilization of corresponding attenuated strains for control purposes. In this study, we utilized cucumber mosaic virus (CMV), a member of the RNA virus group, as the foundational framework for developing attenuated vaccines. We developed four vaccines by inserting relevant sequences from tomato yellow leaf curl virus (TYLCV), a DNA virus. All vaccines demonstrated effective prevention against TYLCV infection, with relative control efficacies exceeding 80%. Subsequently, we evaluated the preventive effects of these vaccines on mixed infections of CMV and TYLCV. Our findings demonstrated that CMV (R2-2bPTI-TYC1C4), CMV (R2-2bPTII-TYC1C4), and CMV (R2-2bPTIII-TYRep) displayed significant efficacy in preventing mixed infections. Following pre-inoculation with these vaccines, the disease index of tomato plants decreased from 100 to 56. This work provides theoretical foundations and tangible resources for controlling TYLCV through cross-protection while suggesting a feasible strategy for utilizing weak RNA virus vaccines to control DNA viruses.
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来源期刊
Agronomy-Basel
Agronomy-Basel Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
6.20
自引率
13.50%
发文量
2665
审稿时长
20.32 days
期刊介绍: Agronomy (ISSN 2073-4395) is an international and cross-disciplinary scholarly journal on agronomy and agroecology. It publishes reviews, regular research papers, communications and short notes, and there is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.
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