IAA对planifolia香草插管的影响,富含磷酸溶剂细菌和钾溶剂细菌

D. E. Kusbianto, Sri Emiyati, T. Setiawati, G. Subroto, Mohammad Ghufron Rosyady
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引用次数: 0

摘要

香草植物的繁殖通常使用插条等营养方法。使用外部生长调节剂可以支持托儿所的生长。磷酸盐溶解菌和钾溶解菌作为促进植物生长的根际细菌产生微生物IAA。本研究将生长素与来自磷酸盐溶解菌和钾溶解菌的IAA微生物组合用于合成IAA。本研究旨在确定每种微生物在每种剂量的合成IAA下的有效性。这项研究于2021年11月至2022年4月在生物土壤实验室和詹伯温室大学进行。该研究采用随机分组设计,三次重复,因子排列。第一个因素是富含细菌分离物的有机物(B0=对照,B1=BPF分离物,B2=BPK分离物)。第二个因素是IAA的浓度(A0=0 mg.l-1,A1=100 mg.l-1和A2=200 mg.l-1)。数据通过ANOVA进行分析,并用Duncan多范围检验(DMRT)进行进一步检验,置信水平为95%。结果表明,合成IAA处理影响不定根的长度和体积参数,以200ppm的浓度为最佳处理有助于提供养分。同时,磷酸盐溶解菌和钾溶解菌的培养基对香草幼苗的卷须长度、叶片数、不定根和主根数、不定根和主茎长度、不定根和主根体积以及鲜重的观察效果优于对照。
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Pengaruh Pemberian IAA pada Pembibitan Setek Vanili (Vanila planifolia) yang diperkaya Bakteri Pelarut Fosfat dan Bakteri Pelarut Kalium
Propagation of vanilla plants is generally using vegetative methods like cuttings. The use of external growth regulators can support the growth of nurseries. Phosphate solubilising bacteria and potassium solubilising bacteria as plant growth promoting rhizobacteria are producing microbial IAA. This study used auxin in synthetic IAA combined with IAA microbes from phosphate solubilising bacteria and potassium solubilising bacteria. This study aimed to determine the effectiveness of each microbe at each dose of synthetic IAA that was applied. The study was conducted from November 2021-April 2022 in the Biological Soil Laboratory and Greenhouse University of Jember. The research uses a randomised block design with three replications with the factorial arrangement. The first factor was organic matter which was enriched with bacterial isolate (B0 = control, B1 = BPF isolate, and B2 = BPK isolate). The second factor was concentration of IAA (A0 = 0 mg.l-1, A1 = 100 mg.l-1, and A2 = 200 mg.l-1). The data were analysed by ANOVA and further tested with Duncan Multiple Range Test (DMRT) with a 95% confidence level. The results showed that treatment of synthetic IAA affects the parameters of length and volume of adventitious roots, which are effective in helping to supply nutrients with the best treatment at a concentration of 200 ppm. Meanwhile, enrichment of media with isolates of phosphate solubilising bacteria and potassium solubilising bacteria gave a better effect than control on the observation of tendril length, number of leaves, number of adventitious roots and taproots, length of adventitious roots and taproots, the volume of adventitious roots and taproot, and fresh weight of vanilla seedlings.
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