异体输血对兔免疫指标的影响

IF 0.5 Q4 BIOLOGY Regulatory Mechanisms in Biosystems Pub Date : 2023-05-24 DOI:10.15421/022343
M. O. Malyuk, O. V. Yehorov, M. A. Kulida
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引用次数: 1

摘要

输血总是与免疫风险有关。在动物中,可能会发生免疫冲突,因为除了红细胞和血浆因子外,许多其他抗原(白细胞、长白细胞)都没有被考虑在内。本出版物的重点是同种异体输血后受体兔血液的形态学变化。通过静脉注射异基因全血对5只临床健康兔进行输血建模,估计体重为5.5mL/kg。研究材料为输血后第3、7和23天采集的血液样本。我们确定,在受体动物的机体中,出现了输血后的白细胞增多。实验动物中白细胞数量的增加没有超过生理参数。全血输注后,受体兔的淋巴细胞和粒细胞数量在实验的23天内没有显著变化。我们记录到,在接受同种异体输血后的第3-23天,受体兔体内的单核细胞数量显著减少。输血后,受体兔的T淋巴细胞百分比在实验研究的第3天和第7天与初始条件相比增加,在第23天下降。异基因输血后受体兔的B淋巴细胞百分比比初始水平增加,表明特异性免疫激活。与初始条件相比,全血输注导致受体中O淋巴细胞的百分比在实验的第3天降低,在实验的7天和23天增加。对异体全血移植进行的研究表明,全血输血在受体动物中会引起一系列复杂的免疫反应。进一步研究的前景是研究同种异体输血对受体动物特异性和非特异性免疫环节参数的影响。
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Effects of allogeneic blood transfusion on the immunity parameters in recipient rabbits
Blood transfusion is always associated with immunological risks. In animals, immune conflicts can occur, because other than erythrocytes and plasma factors, many other antigens (leukocytes, trombocytes) are not accounted for. This publication is focused on morphological changes in blood of recipient rabbits following allogeneic transfusion. Modeling of blood transfusion was performed on five clinically healthy rabbits by intravenous injection of allogeneic whole blood in estimation of 5.5 mL/kg of body weight. The materials for the study were the blood samples, gathered on the 3rd, 7th, and 23th days after the transfusion. We determined that in the organism of the recipient animals, there occurred post-transfusion leukocytosis. Increase in the number of leukocytes in the experimental animals did not exceed the physiological parameters. After whole-blood transfusion, the amount of lymphocytes and granulocytes in the recipient rabbits did not significantly change over 23 days of the experiment. We recorded significant decrease in the amount of monocytes on the 3–23rd day after allogeneic transfusion in the organism of the recipient rabbits. Following the blood transfusion, percentage of T-lymphocytes in the recipient rabbits increased compared with the initial condition on the 3rd and 7th days of the experimental studies, and decreased on the 23rd day. Percentage of B-lymphocytes in the recipient rabbits after the allogeneic blood transfusion increased compared with the initial level, indicating activation of specific immunity. The whole-blood transfusion led to decrease in the percentage of O-lymphocytes in the recipient on the 3rd day of the experiment, and increase on the 7th and 23rd days of the experiment, as compared with the initial condition. The conducted studies of allogeneic transplantation of whole blood demonstrate that whole-blood transfusion entails a cascade of complex immunological reactions in the recipient animals. The prospects for further research are studies of influence of allogeneic transfusion of blood components on the parameters of specific and non-specific links of immunity of the recipient animals.
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来源期刊
CiteScore
0.90
自引率
0.00%
发文量
25
审稿时长
10 weeks
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