Pub Date : 2023-09-01DOI: 10.15421/10.15421/022369
M. Malyuk, O. Yehorov, I. O. Kharkevych, T. L. Savchuk, V. V. Klymchuk
The study of activation of the humoral link of immunity of recipient rabbits following allogeneic transfusion of erythrocyte mass is important for a fundamental understanding of the formation of immunity in model species of animals. We measured the contents of B-lymphocytes, immunoglobulins of M, G, A classes, circulating immune complexes in blood of the recipient rabbits and antibody-dependent cytotoxic activity of lymphocytes. Number of B-lymphocytes in the blood of the rabbits was determined according to Bianco. Immunoglobulin content in blood serum of the rabbits was measured based on the Mancini method. The spectrophotometry method was used to measure circulating immune complexes. The antibody-dependent cytotoxic activity of lymphocytes was identified by the colorimetric method. Modeling of erythrocyte mass transfusion was performed on five clinically healthy rabbits by intravenous injection of allogeneic erythrocyte mass in the dose of 5.5 mL/kg of body weight. The materials for the study were the blood samples, gathered on the 3rd, 7th, and 23rd days after transfusion. We found that the content of B-lymphocytes in blood of the recipient rabbits increased throughout the research: on the 3rd day the content of B lymphocytes, compared with the initial state (11.4%), increased by 1.94 times and was 22.2%; on the 7th day their content increased by 2.22 times and was 25.4%; and on the 23rd day of the experiment the content of lymphocytes increased by 2.46 times, compared with the initial state, equaling 28.6%. The content of IgM on the 3rd day after transfusion of erythrocyte mass increased by 54.9% and was 2.20 ± 0.70 g/L. On the 7th day it increased by 19.0%, equaling 1.69 ± 0.44 g/L, and on the 23rd day the content of immunoglobulins decreased by 54.2% compared with the initial state (1.42 ± 0.18 g/L, intact rabbits), accounting for 0.77 ± 0.25 g/L. The content of immunoglobulins of class G on the 3rd, 7th, and 23rd days of the experiment was nearly the same as in the initial state. The content of class A immunoglobulins decreased 4.16-fold on the 3rd day of the experiment, approaching the control values afterwards. After the transfusion of erythrocyte mass to the recipient rabbits, the content of circulating immune complexes was higher compared with the initial state throughout the research: on the 3rd day the CICs content increased by 2.13 times, measuring 6.40 conventional units, and on the 7th and 23rd days it approached the initial values of the control group. On the 3rd day after transfusion, the antibody-dependent cytotoxic activity of blood lymphocytes of erythrocyte mass of the rabbits increased compared with the initial state (15.9%), measuring 17.3%; on the 7th day it was 19.4%; and on the 23rd day it increased to 27.9%. The results revealed presence of characteristic immunological changes after allogeneic transfusion of erythrocyte mass to the recipient rabbits, which were a consequence of progressive increase in the activity of t
{"title":"The state of the humoral and cellular links of immunity of recipient rabbits following allogeneic transfusion of erythrocyte mass","authors":"M. Malyuk, O. Yehorov, I. O. Kharkevych, T. L. Savchuk, V. V. Klymchuk","doi":"10.15421/10.15421/022369","DOIUrl":"https://doi.org/10.15421/10.15421/022369","url":null,"abstract":"The study of activation of the humoral link of immunity of recipient rabbits following allogeneic transfusion of erythrocyte mass is important for a fundamental understanding of the formation of immunity in model species of animals. We measured the contents of B-lymphocytes, immunoglobulins of M, G, A classes, circulating immune complexes in blood of the recipient rabbits and antibody-dependent cytotoxic activity of lymphocytes. Number of B-lymphocytes in the blood of the rabbits was determined according to Bianco. Immunoglobulin content in blood serum of the rabbits was measured based on the Mancini method. The spectrophotometry method was used to measure circulating immune complexes. The antibody-dependent cytotoxic activity of lymphocytes was identified by the colorimetric method. Modeling of erythrocyte mass transfusion was performed on five clinically healthy rabbits by intravenous injection of allogeneic erythrocyte mass in the dose of 5.5 mL/kg of body weight. The materials for the study were the blood samples, gathered on the 3rd, 7th, and 23rd days after transfusion. We found that the content of B-lymphocytes in blood of the recipient rabbits increased throughout the research: on the 3rd day the content of B lymphocytes, compared with the initial state (11.4%), increased by 1.94 times and was 22.2%; on the 7th day their content increased by 2.22 times and was 25.4%; and on the 23rd day of the experiment the content of lymphocytes increased by 2.46 times, compared with the initial state, equaling 28.6%. The content of IgM on the 3rd day after transfusion of erythrocyte mass increased by 54.9% and was 2.20 ± 0.70 g/L. On the 7th day it increased by 19.0%, equaling 1.69 ± 0.44 g/L, and on the 23rd day the content of immunoglobulins decreased by 54.2% compared with the initial state (1.42 ± 0.18 g/L, intact rabbits), accounting for 0.77 ± 0.25 g/L. The content of immunoglobulins of class G on the 3rd, 7th, and 23rd days of the experiment was nearly the same as in the initial state. The content of class A immunoglobulins decreased 4.16-fold on the 3rd day of the experiment, approaching the control values afterwards. After the transfusion of erythrocyte mass to the recipient rabbits, the content of circulating immune complexes was higher compared with the initial state throughout the research: on the 3rd day the CICs content increased by 2.13 times, measuring 6.40 conventional units, and on the 7th and 23rd days it approached the initial values of the control group. On the 3rd day after transfusion, the antibody-dependent cytotoxic activity of blood lymphocytes of erythrocyte mass of the rabbits increased compared with the initial state (15.9%), measuring 17.3%; on the 7th day it was 19.4%; and on the 23rd day it increased to 27.9%. The results revealed presence of characteristic immunological changes after allogeneic transfusion of erythrocyte mass to the recipient rabbits, which were a consequence of progressive increase in the activity of t","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":"210 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139346602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-28DOI: 10.15421/10.15421/022367
M. Sargsyan
Since ancient times, Armenians have used wild plants in their diet in both fresh and processed forms. Their gastronomic traditions, knowledge about useful plants, and methods of their application have been passed from generation to generation. In this review we analyse the species composition of plants that are used as spices in the national cuisine, how many there are, and what prospects there are for their use. Field surveys were conducted to collect ethnobotanical data of the use of aromatic plants in all of regions in Armenia during the years 2016–2022. 58 informants (42 women and 16 men) were interviewed after obtaining their prior informed oral consent. The informants included farmers, housewives and cooks engaged in preparing the national cuisine. All the informants participated voluntarily and shared their life experience, knowledge received from grandmothers and their professional skills. For the first time, a list of spicy plants of the flora of Armenia which were used in the preparation of national dishes is presented. Some species are no longer used, because classic spices are already available for sale, but some species of plants are still used today as a seasoning in national dishes. In the flora of Armenia there are 142 species of spicy aromatic plants belonging to 115 genera and 46 families. Studies have shown that the largest number of spicy-aromatic plants grow in Central and Southern Armenia (Armeno-Iranian floral province). On the basis of this rich plant resource, it is possible to organize the cultivation of organic spicy plants and spices in order to use them in various branches of the food industry. The recollected knowledge of our ancestors in the area of cheesemaking, winemaking, beermaking, combining centuries of experience with today's new technologies and use of spice plants can be used to develop the country's economy.
{"title":"Usage of spicy aromatic plants of the flora of Armenia in the national cuisine","authors":"M. Sargsyan","doi":"10.15421/10.15421/022367","DOIUrl":"https://doi.org/10.15421/10.15421/022367","url":null,"abstract":"Since ancient times, Armenians have used wild plants in their diet in both fresh and processed forms. Their gastronomic traditions, knowledge about useful plants, and methods of their application have been passed from generation to generation. In this review we analyse the species composition of plants that are used as spices in the national cuisine, how many there are, and what prospects there are for their use. Field surveys were conducted to collect ethnobotanical data of the use of aromatic plants in all of regions in Armenia during the years 2016–2022. 58 informants (42 women and 16 men) were interviewed after obtaining their prior informed oral consent. The informants included farmers, housewives and cooks engaged in preparing the national cuisine. All the informants participated voluntarily and shared their life experience, knowledge received from grandmothers and their professional skills. For the first time, a list of spicy plants of the flora of Armenia which were used in the preparation of national dishes is presented. Some species are no longer used, because classic spices are already available for sale, but some species of plants are still used today as a seasoning in national dishes. In the flora of Armenia there are 142 species of spicy aromatic plants belonging to 115 genera and 46 families. Studies have shown that the largest number of spicy-aromatic plants grow in Central and Southern Armenia (Armeno-Iranian floral province). On the basis of this rich plant resource, it is possible to organize the cultivation of organic spicy plants and spices in order to use them in various branches of the food industry. The recollected knowledge of our ancestors in the area of cheesemaking, winemaking, beermaking, combining centuries of experience with today's new technologies and use of spice plants can be used to develop the country's economy.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":"105 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139348697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-28DOI: 10.15421/10.15421/022366
I. V. Kizub
The discovery of methods for reprogramming adult somatic cells into induced pluripotent stem cells (iPSCs) opens up prospects of developing personalized cell-based therapy options for a variety of human diseases as well as disease modeling and new drug discovery. Like embryonic stem cells, iPSCs can give rise to various cell types of the human body and are amenable to genetic correction. This allows usage of iPSCs in the development of modern therapies for many virtually incurable human diseases. The review summarizes progress in iPSC research in the context of application in the cardiovascular field including modeling cardiovascular disease, drug study, tissue engineering, and perspectives for personalized cardiovascular medicine.
{"title":"Induced pluripotent stem cells for cardiovascular therapeutics: Progress and perspectives","authors":"I. V. Kizub","doi":"10.15421/10.15421/022366","DOIUrl":"https://doi.org/10.15421/10.15421/022366","url":null,"abstract":"The discovery of methods for reprogramming adult somatic cells into induced pluripotent stem cells (iPSCs) opens up prospects of developing personalized cell-based therapy options for a variety of human diseases as well as disease modeling and new drug discovery. Like embryonic stem cells, iPSCs can give rise to various cell types of the human body and are amenable to genetic correction. This allows usage of iPSCs in the development of modern therapies for many virtually incurable human diseases. The review summarizes progress in iPSC research in the context of application in the cardiovascular field including modeling cardiovascular disease, drug study, tissue engineering, and perspectives for personalized cardiovascular medicine.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":"61 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139348718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-27DOI: 10.15421/10.15421/022368
V. Zazharskyi, K. Alifonova, V. Brygadyrenko, N. M. Zazharska, V. P. Goncharenko, V. V. Solomon
The problem of tuberculosis has been relevant for many years due to active spread of the infection pathogen around the globe, in particular in Ukraine. In this article, we determined the epizootic role of the rice weevil (Sitophilus oryzae (Linnaeus, 1763); Coleoptera, Curculionidae) in the spread of Mycobacterium bovis. We identified the effect of the beetle on properties of the pathogen, particularly the changes in morphology, cultural, biochemical, and biological properties after the bacteria had travelled through the body of the rice weevil. To achieve our objectives, we used the museum 100th passage of the virulent strain of M. bovis. We employed microscopic, cultural, biochemical, biological (infecting the biological model), pathoanatomic, histological methods, and PCR studies. The rice weevil is able to retain mycobacteria and release them into their environment for 30 days after becoming infected, with gradual decrease in the number of microbial cells. According to morphology and cultural properties, the pathogen we isolated in the experiment was identical to the initial culture. Enzymatic activity of the bacteria varied. The bacteria that had passed through the intestines of M. bovis were observed to have changes in the biochemical parameters which helped them to adapt to the new environment. We measured the effect of the rice weevil on pathogenicity of M. bovis, isolated directly from the beetle and from grain contaminated with the insects during the experiment. The practical importance of the study consists in expansion of our understanding of the ways M. bovis spreads, identification of effect the rice weevil has on mycobacteria. It also might help in the search for ways to interrupt the chain of tuberculosis transmission – prevention of spread of the disease to favourable areas.
{"title":"The ability of Sitophilus oryzae (Coleoptera, Curculionidae) to transmit Mycobacterium bovis: Morphology, cultural biochemical properties of the bacteria","authors":"V. Zazharskyi, K. Alifonova, V. Brygadyrenko, N. M. Zazharska, V. P. Goncharenko, V. V. Solomon","doi":"10.15421/10.15421/022368","DOIUrl":"https://doi.org/10.15421/10.15421/022368","url":null,"abstract":"The problem of tuberculosis has been relevant for many years due to active spread of the infection pathogen around the globe, in particular in Ukraine. In this article, we determined the epizootic role of the rice weevil (Sitophilus oryzae (Linnaeus, 1763); Coleoptera, Curculionidae) in the spread of Mycobacterium bovis. We identified the effect of the beetle on properties of the pathogen, particularly the changes in morphology, cultural, biochemical, and biological properties after the bacteria had travelled through the body of the rice weevil. To achieve our objectives, we used the museum 100th passage of the virulent strain of M. bovis. We employed microscopic, cultural, biochemical, biological (infecting the biological model), pathoanatomic, histological methods, and PCR studies. The rice weevil is able to retain mycobacteria and release them into their environment for 30 days after becoming infected, with gradual decrease in the number of microbial cells. According to morphology and cultural properties, the pathogen we isolated in the experiment was identical to the initial culture. Enzymatic activity of the bacteria varied. The bacteria that had passed through the intestines of M. bovis were observed to have changes in the biochemical parameters which helped them to adapt to the new environment. We measured the effect of the rice weevil on pathogenicity of M. bovis, isolated directly from the beetle and from grain contaminated with the insects during the experiment. The practical importance of the study consists in expansion of our understanding of the ways M. bovis spreads, identification of effect the rice weevil has on mycobacteria. It also might help in the search for ways to interrupt the chain of tuberculosis transmission – prevention of spread of the disease to favourable areas.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":"51 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139348878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-24DOI: 10.15421/10.15421/022364
Q. S. Basman, I. A. Huda, J. A. Shayma’a
The antifertility effects of ketoconazole can be avoided or diminished by administering nano-selenium-based-antioxidant plant extract simultaneously or sequentially. Using selenium as nanoparticles (SeNPs) is one of the essential methods for enhancing its therapeutic effects and lowering toxicities. This study aimed to analyze the changes made to the parameters of androgens, such as testosterone and gonadotropin hormones: luteinizing hormone and follicle stimulating hormones together with sperm indexes after administration of antioxidant phytofabricated nanoselenium to mitigate oxidative damage brought on by ketoconazole. In brief, 1% weight-per-volume of the extract was loaded into a solution of 10 mM sodium selenite in various ratios on a magnetic stirrer (50 °C, PH 9) in the dark for 12 hours, left for 48 hours and then sent for characterization, which was performed using ultraviolet-visible spectroscopy (UV-vis spectra), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS). Then after the selection of the solution containing the optimal fabricated selenium nanoparticles it was administered to three groups out of seven groups of albino rats with eight animals in each one as follows: Gr. A negative control (no treatment), Gr. B oral ketoconazole 50 mg/kg for fourteen days, Gr. BC, BD, BE1, BE2 and BE3; each one received oral ketoconazole 50 mg/kg for fourteen days followed by: 200 mg/kg Eruca sativa (Gr. BC), 0.5 mg/kg oral sodium selenite (Gr. BD), 0.5 mg/kg/cm2 skin area local nanoselenium (Gr. BE1), 0.25 mg/kg oral nanoselenium (Gr. BE2) and 0.5 mg/kg oral nano selenium (Gr. BE3) respectively for 28 days. After this period, the animals were anesthetized, and plasma testosterone, luteinizing, and follicle stimulating hormones were assessed using Elisa Kit; after that, they were euthanized, and the epididymis of the right testis was carefully removed for evaluation of sperm indices (count, viability, abnormality, and motility). The reduction of selenium ions into PF-SeNPs induced by Eruca sativa extracts at a ratio of (1:2) (Na2SeO3: Eruca sativa) solution was confirmed by the gradual conversion of colour from dark brown to light yellow and then to reddish-orange after the addition of acidic sodium selenite solution and reacting for 12 h. The final reddish-orange colour is the most significant property of nanoparticles. In UV-vis spectroscopy, a strong absorption peak appeared between 268–964 nm with maxima at 268 nm, confirming the formation of nanoselenium. The optimal phytofabricated nanoselenium particles were obtained with a spherical shape, highly stable, and the smallest in size (39.4 nm in diameter) as proved by DLS with Poly Dispersity Index of 0.242 and zeta potential value of –56.57 mV. In the current study, testicles were damaged by administering ketoconazole at a dose of 50 mg/kg/day orally for 14 days. This testicular damage was linked to significant reductions in testosterone levels, elevated levels
{"title":"Screening the possible effect of a phytofabricated nanoselenium-composite from Eruca sativa extract in reducing infertility in males","authors":"Q. S. Basman, I. A. Huda, J. A. Shayma’a","doi":"10.15421/10.15421/022364","DOIUrl":"https://doi.org/10.15421/10.15421/022364","url":null,"abstract":"The antifertility effects of ketoconazole can be avoided or diminished by administering nano-selenium-based-antioxidant plant extract simultaneously or sequentially. Using selenium as nanoparticles (SeNPs) is one of the essential methods for enhancing its therapeutic effects and lowering toxicities. This study aimed to analyze the changes made to the parameters of androgens, such as testosterone and gonadotropin hormones: luteinizing hormone and follicle stimulating hormones together with sperm indexes after administration of antioxidant phytofabricated nanoselenium to mitigate oxidative damage brought on by ketoconazole. In brief, 1% weight-per-volume of the extract was loaded into a solution of 10 mM sodium selenite in various ratios on a magnetic stirrer (50 °C, PH 9) in the dark for 12 hours, left for 48 hours and then sent for characterization, which was performed using ultraviolet-visible spectroscopy (UV-vis spectra), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS). Then after the selection of the solution containing the optimal fabricated selenium nanoparticles it was administered to three groups out of seven groups of albino rats with eight animals in each one as follows: Gr. A negative control (no treatment), Gr. B oral ketoconazole 50 mg/kg for fourteen days, Gr. BC, BD, BE1, BE2 and BE3; each one received oral ketoconazole 50 mg/kg for fourteen days followed by: 200 mg/kg Eruca sativa (Gr. BC), 0.5 mg/kg oral sodium selenite (Gr. BD), 0.5 mg/kg/cm2 skin area local nanoselenium (Gr. BE1), 0.25 mg/kg oral nanoselenium (Gr. BE2) and 0.5 mg/kg oral nano selenium (Gr. BE3) respectively for 28 days. After this period, the animals were anesthetized, and plasma testosterone, luteinizing, and follicle stimulating hormones were assessed using Elisa Kit; after that, they were euthanized, and the epididymis of the right testis was carefully removed for evaluation of sperm indices (count, viability, abnormality, and motility). The reduction of selenium ions into PF-SeNPs induced by Eruca sativa extracts at a ratio of (1:2) (Na2SeO3: Eruca sativa) solution was confirmed by the gradual conversion of colour from dark brown to light yellow and then to reddish-orange after the addition of acidic sodium selenite solution and reacting for 12 h. The final reddish-orange colour is the most significant property of nanoparticles. In UV-vis spectroscopy, a strong absorption peak appeared between 268–964 nm with maxima at 268 nm, confirming the formation of nanoselenium. The optimal phytofabricated nanoselenium particles were obtained with a spherical shape, highly stable, and the smallest in size (39.4 nm in diameter) as proved by DLS with Poly Dispersity Index of 0.242 and zeta potential value of –56.57 mV. In the current study, testicles were damaged by administering ketoconazole at a dose of 50 mg/kg/day orally for 14 days. This testicular damage was linked to significant reductions in testosterone levels, elevated levels","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":"36 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139349321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-23DOI: 10.15421/10.15421/022363
Y. Bilonozhko, T. Shut, T. Krupodorova, N. Pirko, O. Holubchak, S. Pryvalikhin, O. Lykholat, Y. Pirko
The phytochemical and pharmacological potential of Viscum album extracts are widely used for the treatment of various diseases in many countries. The effect of aqueous extract from different mistletoe host trees (Abies alba, Acer saccharinum, Malus domestica, Pinus sylvestris) on model objects (Allium cepa and Drosophila milanogaster), bacteria (Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus), and fungi (Aspergillus niger, Heterobasidion annosum, Inonotus obliquus) was evaluated. The influence of mistletoe extracts on A. cepa, D. melanogaster and basidiomycetes species H. annosum and I. obliquus was demonstrated for the first time. The study findings revealed that the impact of mistletoe aqueous extract exhibits considerable variation, influenced by multiple factors. These factors include the specific tree from which mistletoe is obtained, the concentration used, and the organism targeted. Mistletoe extracts caused the appearance of cells with pathologies in the meristematic tissues of A. cepa at different stages of the plant cell cycle. A clear cytostatic effect on A. cepa and D. melanogaster was shown, with the highest effect of mistletoe extract obtained from A. saccharinum. The main action of the solution on cells is probably manifested in the inhibition of cell division according to the results of the cytotoxicity assessment of aqueous extracts of mistletoe. Antibacterial activity of concentated mistletoe extract obtained from A. saccharinum was found only against S. aureus with zone of growth inhibition 11.0 ± 0.1 mm. In the assay on agar plates the tested extracts stimulated the mycelial growth of A. niger 1.85 times higher compared to the control, but inhibited the growth of H. annosum, I. obliquus at 65.5% and 62.2%, respectively. However, the biomass production of H. annosum was strongly dependent on the host mistletoe tree of the aqueous extract, and both the inhibition of mycelial synthesis of the wood-destroying fungus and its stimulating effect on mycelial accumulation were found. A negative effect (1.46 times decrease in biomass ) of H. annosum was found in the case of application of mistletoe extract obtained from A. saccharinum. The highest stimulation effect on fungus (1.53 times increase in biomass) was established by using mistletoe extract prepared from A. alba. In this study the main effects on living organisms used were indirectly caused by the host trees of mistletoe extracts. The overall negative impact was associated with the host tree Acer saccharinum.
在许多国家，槲寄生提取物的植物化学成分和药理潜力被广泛用于治疗各种疾病。本研究评估了不同槲寄生寄主树（白枞树、糖槭树、马栗树、欧洲赤松）的水提取物对模型对象（牛肝菌和果蝇）、细菌（大肠杆菌、肺炎克雷伯氏菌、金黄色葡萄球菌）和真菌（黑曲霉、年轮异菌、钝顶稻曲霉）的影响。研究首次证明了槲寄生提取物对牛肝菌、黑曲霉和基枝菌 H. annosum 和 I. obliquus 的影响。研究结果表明，槲寄生水提取物的影响受多种因素的影响，表现出相当大的差异。这些因素包括提取槲寄生水提取物的特定树种、使用的浓度和目标生物。在植物细胞周期的不同阶段，槲寄生提取物会导致牛肝菌的分生组织出现病变细胞。结果表明，槲寄生提取物对 A. cepa 和 D. melanogaster 有明显的细胞抑制作用，其中以 A. saccharinum 提取物的作用最大。根据槲寄生水提取物的细胞毒性评估结果，溶液对细胞的主要作用可能表现为抑制细胞分裂。从 A. saccharinum 中提取的浓缩槲寄生提取物只对金黄色葡萄球菌具有抗菌活性，生长抑制区为 11.0 ± 0.1 毫米。在琼脂平板上进行的试验中，与对照组相比，受试提取物对黑僵菌菌丝生长的刺激作用高出 1.85 倍，但对 H. annosum 和 I. obliquus 的生长抑制率分别为 65.5% 和 62.2%。不过，H. annosum 的生物量产生与水提取物的寄主槲寄生树有很大关系，既有抑制蛀木真菌菌丝合成的作用，也有刺激菌丝积累的作用。在施用从 A. saccharinum 中提取的槲寄生提取物的情况下，H. annosum 受到了负面影响（生物量减少了 1.46 倍）。使用白叶槲寄生提取物对真菌的刺激作用最大（生物量增加 1.53 倍）。在这项研究中，槲寄生提取物对生物的主要影响是由寄主植物间接造成的。总体的负面影响与寄主树 Acer saccharinum 有关。
{"title":"Impact of aqueous extract of Viscum album on different organisms","authors":"Y. Bilonozhko, T. Shut, T. Krupodorova, N. Pirko, O. Holubchak, S. Pryvalikhin, O. Lykholat, Y. Pirko","doi":"10.15421/10.15421/022363","DOIUrl":"https://doi.org/10.15421/10.15421/022363","url":null,"abstract":"The phytochemical and pharmacological potential of Viscum album extracts are widely used for the treatment of various diseases in many countries. The effect of aqueous extract from different mistletoe host trees (Abies alba, Acer saccharinum, Malus domestica, Pinus sylvestris) on model objects (Allium cepa and Drosophila milanogaster), bacteria (Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus), and fungi (Aspergillus niger, Heterobasidion annosum, Inonotus obliquus) was evaluated. The influence of mistletoe extracts on A. cepa, D. melanogaster and basidiomycetes species H. annosum and I. obliquus was demonstrated for the first time. The study findings revealed that the impact of mistletoe aqueous extract exhibits considerable variation, influenced by multiple factors. These factors include the specific tree from which mistletoe is obtained, the concentration used, and the organism targeted. Mistletoe extracts caused the appearance of cells with pathologies in the meristematic tissues of A. cepa at different stages of the plant cell cycle. A clear cytostatic effect on A. cepa and D. melanogaster was shown, with the highest effect of mistletoe extract obtained from A. saccharinum. The main action of the solution on cells is probably manifested in the inhibition of cell division according to the results of the cytotoxicity assessment of aqueous extracts of mistletoe. Antibacterial activity of concentated mistletoe extract obtained from A. saccharinum was found only against S. aureus with zone of growth inhibition 11.0 ± 0.1 mm. In the assay on agar plates the tested extracts stimulated the mycelial growth of A. niger 1.85 times higher compared to the control, but inhibited the growth of H. annosum, I. obliquus at 65.5% and 62.2%, respectively. However, the biomass production of H. annosum was strongly dependent on the host mistletoe tree of the aqueous extract, and both the inhibition of mycelial synthesis of the wood-destroying fungus and its stimulating effect on mycelial accumulation were found. A negative effect (1.46 times decrease in biomass ) of H. annosum was found in the case of application of mistletoe extract obtained from A. saccharinum. The highest stimulation effect on fungus (1.53 times increase in biomass) was established by using mistletoe extract prepared from A. alba. In this study the main effects on living organisms used were indirectly caused by the host trees of mistletoe extracts. The overall negative impact was associated with the host tree Acer saccharinum.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":"147 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139349472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-23DOI: 10.15421/10.15421/022372
B. Verveha, B. Gutyj, M. I. Holubiev, M. M. Kondro, І. V. Dats
In the pathogenesis of acute widespread peritonitis and accompanying diabetes, a vital link is an endogenous intoxication caused by the translocation of microorganisms and their toxins from the intestine into the blood, metabolic changes, and immunological reactivity of the body. Our work aimed to investigate the microbial composition in the parietal intestinal biotope and the features of the blood cytokine profile in animals with acute disseminated peritonitis on the background of streptozotocin-induced diabetes. The study was conducted on 56 sexually mature non-linear white male rats. Diabetes mellitus was modeled by a single intraperitoneal injection of streptozotocin (60 mg/kg). On the 14th day of the development of streptozotocin-induced diabetes mellitus, a 10% filtered fecal suspension (0.5 mL) was injected into the abdominal cavity of animals, and acute generalized peritonitis was initiated. Sowing on nutrient media was carried out for bacteriological research to isolate a pure culture of microorganisms and their identification. The concentration of TNF-α, IL-1β, and IL-6 was studied by solid-phase enzyme immunoassay. The research results demonstrate an imbalance of cytokines in the dynamics of experimental acute disseminated peritonitis against the background of diabetes and quantitative and qualitative changes in the microbiota of the parietal intestinal biotope. A decrease in the number of Escherichia coli strains isolated in monoculture and an increase in the number of two-component and three-component microbial associations were revealed, among which Enterobacter aerogenes, Escherichia coli, Bacteroides spp., Proteus mirabilis, Klebsiella spp. and Candida species prevailed.
{"title":"Gut microbiota and changes in cytokine profile in animals with experimental acute disseminated peritonitis on the background of diabetes","authors":"B. Verveha, B. Gutyj, M. I. Holubiev, M. M. Kondro, І. V. Dats","doi":"10.15421/10.15421/022372","DOIUrl":"https://doi.org/10.15421/10.15421/022372","url":null,"abstract":"In the pathogenesis of acute widespread peritonitis and accompanying diabetes, a vital link is an endogenous intoxication caused by the translocation of microorganisms and their toxins from the intestine into the blood, metabolic changes, and immunological reactivity of the body. Our work aimed to investigate the microbial composition in the parietal intestinal biotope and the features of the blood cytokine profile in animals with acute disseminated peritonitis on the background of streptozotocin-induced diabetes. The study was conducted on 56 sexually mature non-linear white male rats. Diabetes mellitus was modeled by a single intraperitoneal injection of streptozotocin (60 mg/kg). On the 14th day of the development of streptozotocin-induced diabetes mellitus, a 10% filtered fecal suspension (0.5 mL) was injected into the abdominal cavity of animals, and acute generalized peritonitis was initiated. Sowing on nutrient media was carried out for bacteriological research to isolate a pure culture of microorganisms and their identification. The concentration of TNF-α, IL-1β, and IL-6 was studied by solid-phase enzyme immunoassay. The research results demonstrate an imbalance of cytokines in the dynamics of experimental acute disseminated peritonitis against the background of diabetes and quantitative and qualitative changes in the microbiota of the parietal intestinal biotope. A decrease in the number of Escherichia coli strains isolated in monoculture and an increase in the number of two-component and three-component microbial associations were revealed, among which Enterobacter aerogenes, Escherichia coli, Bacteroides spp., Proteus mirabilis, Klebsiella spp. and Candida species prevailed.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":"60 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139349384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-23DOI: 10.15421/10.15421/022362
V. Vlizlo, O. Prystupa, L. Slivinska, B. Gutyj, I. Maksymovych, A. Shcherbatyy, M. Lychuk, U. Partyka, B. Chernushkin, V. Rusyn, M. Leno, K. Leskiv
Medicinal plants are a source of various therapeutic preparations. Therefore, the aim of our work was to prepare a liposomal drug from extract from the seeds of Silybum marianum (L.) Gaertn., adding tocopherol acetate, lecithin, squalene, and Tween 80. The drug was used on the laboratory animals (rats) intramuscularly to measure the efficacy of treatment of experimentally modeled toxic fatty liver disease. The fatty infiltration of the liver in the rats was caused by tetrachloromethane (ССl4). The efficacy of the liposomal drug based on the extract from S. marianum seeds was studied on 25 animals in which the liver pathology had been caused by 50% oil solution of ССl4, administered in the dose of 5 mL per kg of body weight. The diseased rats were divided into five groups, each consisting five animals. Animals of the four experimental groups – first, second, third, and fourth - received the drug intramuscularly in the doses of 0.05, 0.25, 0.50, and 1.50 mL/kg of body weight three times every two days, respectively. At the same time, the control rats received three-time intramuscular injection of physiological solution in the dose of 0.5 mL/kg of body weight. Treatment of the animals with fatty liver disease by injections of the drug based on the extract from S. marianum seeds normalized the general condition, significantly improved the functions and structure of the liver. Biochemical studies of blood serum of the sick animals after the treatment revealed increase in albumin content, which may suggest reduction of the protein-synthesizing function of the liver. The normalization of the bile-forming and bile-excreting functions of the liver, and also elimination of cholestasis were evidenced by reduced contents of bile acids and total bilirubin and increased total cholesterol in the blood serum of the rats. After treating the animals with the created drug, we saw decrease in the activity of the liver-indicator enzymes (aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltransferase, and glutamate dehydrogenase) in the blood serum, which is a sign of recovery of the structure of hepatocytes and elimination of cytolysis. Histological studies of the liver of the treated rats confirmed the positive effect of the liposomal drug on the organ’s structure. In the future studies, we plan to test this combination of agents in treatment of agricultural and domestic animals with liver pathologies.
{"title":"Treatment of animals with fatty liver disease using a drug based on the seeds of Silybum marianum","authors":"V. Vlizlo, O. Prystupa, L. Slivinska, B. Gutyj, I. Maksymovych, A. Shcherbatyy, M. Lychuk, U. Partyka, B. Chernushkin, V. Rusyn, M. Leno, K. Leskiv","doi":"10.15421/10.15421/022362","DOIUrl":"https://doi.org/10.15421/10.15421/022362","url":null,"abstract":"Medicinal plants are a source of various therapeutic preparations. Therefore, the aim of our work was to prepare a liposomal drug from extract from the seeds of Silybum marianum (L.) Gaertn., adding tocopherol acetate, lecithin, squalene, and Tween 80. The drug was used on the laboratory animals (rats) intramuscularly to measure the efficacy of treatment of experimentally modeled toxic fatty liver disease. The fatty infiltration of the liver in the rats was caused by tetrachloromethane (ССl4). The efficacy of the liposomal drug based on the extract from S. marianum seeds was studied on 25 animals in which the liver pathology had been caused by 50% oil solution of ССl4, administered in the dose of 5 mL per kg of body weight. The diseased rats were divided into five groups, each consisting five animals. Animals of the four experimental groups – first, second, third, and fourth - received the drug intramuscularly in the doses of 0.05, 0.25, 0.50, and 1.50 mL/kg of body weight three times every two days, respectively. At the same time, the control rats received three-time intramuscular injection of physiological solution in the dose of 0.5 mL/kg of body weight. Treatment of the animals with fatty liver disease by injections of the drug based on the extract from S. marianum seeds normalized the general condition, significantly improved the functions and structure of the liver. Biochemical studies of blood serum of the sick animals after the treatment revealed increase in albumin content, which may suggest reduction of the protein-synthesizing function of the liver. The normalization of the bile-forming and bile-excreting functions of the liver, and also elimination of cholestasis were evidenced by reduced contents of bile acids and total bilirubin and increased total cholesterol in the blood serum of the rats. After treating the animals with the created drug, we saw decrease in the activity of the liver-indicator enzymes (aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltransferase, and glutamate dehydrogenase) in the blood serum, which is a sign of recovery of the structure of hepatocytes and elimination of cytolysis. Histological studies of the liver of the treated rats confirmed the positive effect of the liposomal drug on the organ’s structure. In the future studies, we plan to test this combination of agents in treatment of agricultural and domestic animals with liver pathologies.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":"65 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139349463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-23DOI: 10.15421/10.15421/022365
N. Bakhshaliyeva, K. Aliyeva, J. Mammadov, A. Hummatov
The relevance of the problem under study lies in the need to develop innovative approaches to the introduction of fruit crops into the diet to avoid the loss of nutrients and thus help prevent micronutrient deficiency in the human body. In this regard, the purpose of this article is to study the content of biologically active substances in the products of promising persimmon varieties and their effect on the normal functioning of the thyroid gland. The following results were obtained during the study: soil and climatic conditions affecting the nutritional value of the agricultural product were presented. It was established that Shaki-Zagatala economic region is an iodine deficiency endemic area and belongs to the zone of moderate iodine (I) deficiency. The quantity of macro- and microelements in fruits of 20 varieties of Oriental persimmon (Diospyros kaki) bred by folk selection growing in Shaki-Zagatala economic region was studied and compared by regions; the use of persimmon plants in the food industry as a way of increasing the amount of iodine in the human body was substantiated. It has been proved that in order to eliminate iodine deficiency, it is advantageous to eat more food enriched with iodine from fruits of the Oriental persimmon (D. kaki), which is rich in microelements. The role of awareness – raising activities among the population regarding health disorders associated with iodine deficiency was revealed. The practical value of the article materials consists in obtaining detailed information about biochemical composition of Oriental persimmon fruit (D. kaki) for the purpose of iodine deficiency prevention. The technologies of processing and storage of the product in fresh and processed form to preserve the useful properties of persimmon have been proposed in order to increase the profitability of production and increase the level of sales of products in the Shaki-Zagatala economic region of Azerbaijan.
{"title":"The content of microelements in fruits of the oriental persimmon (Diospyros kaki) and its dietary role in remedying micronutrient deficiency in the region","authors":"N. Bakhshaliyeva, K. Aliyeva, J. Mammadov, A. Hummatov","doi":"10.15421/10.15421/022365","DOIUrl":"https://doi.org/10.15421/10.15421/022365","url":null,"abstract":"The relevance of the problem under study lies in the need to develop innovative approaches to the introduction of fruit crops into the diet to avoid the loss of nutrients and thus help prevent micronutrient deficiency in the human body. In this regard, the purpose of this article is to study the content of biologically active substances in the products of promising persimmon varieties and their effect on the normal functioning of the thyroid gland. The following results were obtained during the study: soil and climatic conditions affecting the nutritional value of the agricultural product were presented. It was established that Shaki-Zagatala economic region is an iodine deficiency endemic area and belongs to the zone of moderate iodine (I) deficiency. The quantity of macro- and microelements in fruits of 20 varieties of Oriental persimmon (Diospyros kaki) bred by folk selection growing in Shaki-Zagatala economic region was studied and compared by regions; the use of persimmon plants in the food industry as a way of increasing the amount of iodine in the human body was substantiated. It has been proved that in order to eliminate iodine deficiency, it is advantageous to eat more food enriched with iodine from fruits of the Oriental persimmon (D. kaki), which is rich in microelements. The role of awareness – raising activities among the population regarding health disorders associated with iodine deficiency was revealed. The practical value of the article materials consists in obtaining detailed information about biochemical composition of Oriental persimmon fruit (D. kaki) for the purpose of iodine deficiency prevention. The technologies of processing and storage of the product in fresh and processed form to preserve the useful properties of persimmon have been proposed in order to increase the profitability of production and increase the level of sales of products in the Shaki-Zagatala economic region of Azerbaijan.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":"2015 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139349497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-20DOI: 10.15421/10.15421/022370
V. Langraf, A. Svoradová, K. Petrovičová, V. Brygadyrenko
Bioinformatics is a scientific field on the border between informatics and biology where problems in the field of biology are solved using statistical methods. Another part of it are database systems which serve to store data necessary for meta-analysis. In recent years, there has been a boom mainly thanks to enabling technologies that make it possible to obtain big data about the functioning of living cells of organisms. Bioinformatics tools are necessary to process these data and form an integral part of research in modern biological and medical sciences. Scientific research focused on molecular biology, as well as medicine, is increasingly focusing on data storage. It is understood that the correct structure of the database is important for the correct interpretation of the results of their research activities. For communication between tables in the database, it is essential to set the data type, assign Primary key and Foreign key, ensure data integrity, remove data plurality and understand the research logic. Based on these needs, we created a relational database using SQL Server 2017 and Microsoft SQL Server Management Studio 2017 (SSMS). We created the source code for programming the database and filling it with data in Structured Query Language (SQL) and T-SQL on the Microsoft platform. Of the data types, we used float for numbers with a floating decimal line, integer values were assigned an integer (int), date had a date data type, and text strings had a defined nvarchar data type. Our results bring new information in the field of bioinformatics about the creation of a database structure for data storage in cell biology research. These new insights will help big data in meta-analyses of data and applying scientific results to medical and scientific practice. The database will store data obtained in real time, which will ensure relevance in pointing out biological trends, regularities, relationships and links between cellular structures. All these aspects are very important for the spatial modeling of data and the creation of models of interactions of cell structures with use for applications in medical and biological practice.
生物信息学是介于信息学和生物学之间的一个科学领域，在这一领域中，生物学领域的问题通过统计方法得以解决。它的另一部分是数据库系统，用于存储元分析所需的数据。近年来，生物信息学的蓬勃发展主要归功于使获取生物活细胞功能大数据成为可能的使能技术。生物信息学工具是处理这些数据的必要手段，也是现代生物和医学研究不可或缺的一部分。以分子生物学和医学为重点的科学研究越来越重视数据存储。据了解，数据库的正确结构对于正确解释其研究活动的结果非常重要。为了实现数据库表之间的通信，必须设置数据类型、分配主键和外键、确保数据完整性、消除数据多重性并了解研究逻辑。根据这些需求，我们使用 SQL Server 2017 和 Microsoft SQL Server Management Studio 2017（SSMS）创建了一个关系数据库。我们在微软平台上使用结构化查询语言（SQL）和 T-SQL 创建了源代码，用于对数据库进行编程并填充数据。在数据类型中，我们对带有浮动小数行的数字使用了 float，对整数值分配了整数（int），对日期使用了日期数据类型，对文本字符串使用了定义好的 nvarchar 数据类型。我们的研究结果为生物信息学领域带来了新的信息，即在细胞生物学研究中创建用于数据存储的数据库结构。这些新见解将有助于大数据的数据荟萃分析，并将科学成果应用于医疗和科学实践。数据库将存储实时获得的数据，这将确保在指出生物学趋势、规律性、细胞结构之间的关系和联系方面的相关性。所有这些方面对于数据的空间建模和细胞结构相互作用模型的创建都非常重要，可用于医学和生物学实践。
{"title":"Structure of data in cell biology research","authors":"V. Langraf, A. Svoradová, K. Petrovičová, V. Brygadyrenko","doi":"10.15421/10.15421/022370","DOIUrl":"https://doi.org/10.15421/10.15421/022370","url":null,"abstract":"Bioinformatics is a scientific field on the border between informatics and biology where problems in the field of biology are solved using statistical methods. Another part of it are database systems which serve to store data necessary for meta-analysis. In recent years, there has been a boom mainly thanks to enabling technologies that make it possible to obtain big data about the functioning of living cells of organisms. Bioinformatics tools are necessary to process these data and form an integral part of research in modern biological and medical sciences. Scientific research focused on molecular biology, as well as medicine, is increasingly focusing on data storage. It is understood that the correct structure of the database is important for the correct interpretation of the results of their research activities. For communication between tables in the database, it is essential to set the data type, assign Primary key and Foreign key, ensure data integrity, remove data plurality and understand the research logic. Based on these needs, we created a relational database using SQL Server 2017 and Microsoft SQL Server Management Studio 2017 (SSMS). We created the source code for programming the database and filling it with data in Structured Query Language (SQL) and T-SQL on the Microsoft platform. Of the data types, we used float for numbers with a floating decimal line, integer values were assigned an integer (int), date had a date data type, and text strings had a defined nvarchar data type. Our results bring new information in the field of bioinformatics about the creation of a database structure for data storage in cell biology research. These new insights will help big data in meta-analyses of data and applying scientific results to medical and scientific practice. The database will store data obtained in real time, which will ensure relevance in pointing out biological trends, regularities, relationships and links between cellular structures. All these aspects are very important for the spatial modeling of data and the creation of models of interactions of cell structures with use for applications in medical and biological practice.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":"39 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139349938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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