可见光辅助酶催化级联降解有机磷农药

IF 9.1 Q1 ENGINEERING, CHEMICAL Green Chemical Engineering Pub Date : 2023-03-01 DOI:10.1016/j.gce.2022.02.001
Ying Zhang , Xue Cao , Yufeng Yang , Sumin Guan , Xiaotian Wang , Heyu Li , Xiaobing Zheng , Liya Zhou , Yanjun Jiang , Jing Gao
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引用次数: 8

摘要

有机磷农药在世界范围内的应用促进了农业发展,但其在土壤和水中的逐渐积累会严重影响人类和其他哺乳动物的中枢神经系统。有机磷水解酶(OPH)是一种有效的酶,可以催化降解残留的有机磷。然而,降解产物如对硝基苯酚(p-NP)仍然有毒。因此,开发一种可同时用于固定OPH和进一步降解p-NP的多功能载体具有重要意义。本文通过将OPH固定在中空结构的Au-TiO2(命名为OPH@H-Au-TiO2)用于OP的降解。获得的OPH@H-Au-TiO2在可见光条件下,可以用OPH将甲基对硫磷降解为p-NP,再用H-Au-TiO2将p-NP降解为低毒对苯二酚。采用吸附法将OPH分子固定在H-Au-TiO2上制备OPH@H-Au-TiO2.反应2.5小时后,甲基对硫磷被完全降解,约82.64%的生成的p-NP被进一步降解为对苯二酚。重复使用4次后OPH@H-Au-TiO2保留了80%以上的初始降解活性。本研究为设计和构建多功能生物催化剂提供了新的见解,极大地拓展了酶催化的应用场景和工业价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Visible light assisted enzyme-photocatalytic cascade degradation of organophosphorus pesticides

The worldwide application of organophosphorus pesticides (OPs) has promoted agricultural development, but their gradual accumulation in soil and water can seriously affect the central nervous system of humans and other mammals. Organophosphorus hydrolase (OPH) is an effective enzyme that can catalyze the degradation of the residual OPs. However, the degradation products such as p-nitrophenol (p-NP) is still toxic. Thus, it is of great significance to develop a multi-functional support that can be simultaneously used for the immobilization of OPH and the further degradation of p-NP. Herein, a visible light assisted enzyme-photocatalytic integrated catalyst was constructed by immobilizing OPH on hollow structured Au-TiO2 (named OPH@H-Au-TiO2) for the degradation of OPs. The obtained OPH@H-Au-TiO2 can degrade methyl parathion to p-NP by OPH and then degrade p-NP to hydroquinone with low toxicity by using H-Au-TiO2 under visible light. OPH molecules were immobilized on H-Au-TiO2 through adsorption method to prepare OPH@H-Au-TiO2. After 2.5 h of reaction, methyl parathion is completely degraded, and about 82.64% of the generated p-NP is further degraded into hydroquinone. After reused for 4 times, the OPH@H-Au-TiO2 retains more than 80% of the initial degradation activity. This research presents a new insight in designing and constructing multi-functional biocatalyst, which greatly expands the application scenarios and industrial value of enzyme catalysis.

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来源期刊
Green Chemical Engineering
Green Chemical Engineering Process Chemistry and Technology, Catalysis, Filtration and Separation
CiteScore
11.60
自引率
0.00%
发文量
58
审稿时长
51 days
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