可见光辅助酶催化级联降解有机磷农药

IF 9.1 Q1 ENGINEERING, CHEMICAL Green Chemical Engineering Pub Date : 2023-03-01 DOI:10.1016/j.gce.2022.02.001
Ying Zhang , Xue Cao , Yufeng Yang , Sumin Guan , Xiaotian Wang , Heyu Li , Xiaobing Zheng , Liya Zhou , Yanjun Jiang , Jing Gao
{"title":"可见光辅助酶催化级联降解有机磷农药","authors":"Ying Zhang ,&nbsp;Xue Cao ,&nbsp;Yufeng Yang ,&nbsp;Sumin Guan ,&nbsp;Xiaotian Wang ,&nbsp;Heyu Li ,&nbsp;Xiaobing Zheng ,&nbsp;Liya Zhou ,&nbsp;Yanjun Jiang ,&nbsp;Jing Gao","doi":"10.1016/j.gce.2022.02.001","DOIUrl":null,"url":null,"abstract":"<div><p>The worldwide application of organophosphorus pesticides (OPs) has promoted agricultural development, but their gradual accumulation in soil and water can seriously affect the central nervous system of humans and other mammals. Organophosphorus hydrolase (OPH) is an effective enzyme that can catalyze the degradation of the residual OPs. However, the degradation products such as <em>p</em>-nitrophenol (<em>p</em>-NP) is still toxic. Thus, it is of great significance to develop a multi-functional support that can be simultaneously used for the immobilization of OPH and the further degradation of <em>p</em>-NP. Herein, a visible light assisted enzyme-photocatalytic integrated catalyst was constructed by immobilizing OPH on hollow structured Au-TiO<sub>2</sub> (named OPH@H-Au-TiO<sub>2</sub>) for the degradation of OPs. The obtained OPH@H-Au-TiO<sub>2</sub> can degrade methyl parathion to <em>p</em>-NP by OPH and then degrade <em>p</em>-NP to hydroquinone with low toxicity by using H-Au-TiO<sub>2</sub> under visible light. OPH molecules were immobilized on H-Au-TiO<sub>2</sub> through adsorption method to prepare OPH@H-Au-TiO<sub>2</sub>. After 2.5 h of reaction, methyl parathion is completely degraded, and about 82.64% of the generated <em>p</em>-NP is further degraded into hydroquinone. After reused for 4 times, the OPH@H-Au-TiO<sub>2</sub> retains more than 80% of the initial degradation activity. This research presents a new insight in designing and constructing multi-functional biocatalyst, which greatly expands the application scenarios and industrial value of enzyme catalysis.</p></div>","PeriodicalId":66474,"journal":{"name":"Green Chemical Engineering","volume":null,"pages":null},"PeriodicalIF":9.1000,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"8","resultStr":"{\"title\":\"Visible light assisted enzyme-photocatalytic cascade degradation of organophosphorus pesticides\",\"authors\":\"Ying Zhang ,&nbsp;Xue Cao ,&nbsp;Yufeng Yang ,&nbsp;Sumin Guan ,&nbsp;Xiaotian Wang ,&nbsp;Heyu Li ,&nbsp;Xiaobing Zheng ,&nbsp;Liya Zhou ,&nbsp;Yanjun Jiang ,&nbsp;Jing Gao\",\"doi\":\"10.1016/j.gce.2022.02.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The worldwide application of organophosphorus pesticides (OPs) has promoted agricultural development, but their gradual accumulation in soil and water can seriously affect the central nervous system of humans and other mammals. Organophosphorus hydrolase (OPH) is an effective enzyme that can catalyze the degradation of the residual OPs. However, the degradation products such as <em>p</em>-nitrophenol (<em>p</em>-NP) is still toxic. Thus, it is of great significance to develop a multi-functional support that can be simultaneously used for the immobilization of OPH and the further degradation of <em>p</em>-NP. Herein, a visible light assisted enzyme-photocatalytic integrated catalyst was constructed by immobilizing OPH on hollow structured Au-TiO<sub>2</sub> (named OPH@H-Au-TiO<sub>2</sub>) for the degradation of OPs. The obtained OPH@H-Au-TiO<sub>2</sub> can degrade methyl parathion to <em>p</em>-NP by OPH and then degrade <em>p</em>-NP to hydroquinone with low toxicity by using H-Au-TiO<sub>2</sub> under visible light. OPH molecules were immobilized on H-Au-TiO<sub>2</sub> through adsorption method to prepare OPH@H-Au-TiO<sub>2</sub>. After 2.5 h of reaction, methyl parathion is completely degraded, and about 82.64% of the generated <em>p</em>-NP is further degraded into hydroquinone. After reused for 4 times, the OPH@H-Au-TiO<sub>2</sub> retains more than 80% of the initial degradation activity. This research presents a new insight in designing and constructing multi-functional biocatalyst, which greatly expands the application scenarios and industrial value of enzyme catalysis.</p></div>\",\"PeriodicalId\":66474,\"journal\":{\"name\":\"Green Chemical Engineering\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":9.1000,\"publicationDate\":\"2023-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Green Chemical Engineering\",\"FirstCategoryId\":\"1089\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2666952822000164\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, CHEMICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Green Chemical Engineering","FirstCategoryId":"1089","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666952822000164","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}
引用次数: 8

摘要

有机磷农药在世界范围内的应用促进了农业发展,但其在土壤和水中的逐渐积累会严重影响人类和其他哺乳动物的中枢神经系统。有机磷水解酶(OPH)是一种有效的酶,可以催化降解残留的有机磷。然而,降解产物如对硝基苯酚(p-NP)仍然有毒。因此,开发一种可同时用于固定OPH和进一步降解p-NP的多功能载体具有重要意义。本文通过将OPH固定在中空结构的Au-TiO2(命名为OPH@H-Au-TiO2)用于OP的降解。获得的OPH@H-Au-TiO2在可见光条件下,可以用OPH将甲基对硫磷降解为p-NP,再用H-Au-TiO2将p-NP降解为低毒对苯二酚。采用吸附法将OPH分子固定在H-Au-TiO2上制备OPH@H-Au-TiO2.反应2.5小时后,甲基对硫磷被完全降解,约82.64%的生成的p-NP被进一步降解为对苯二酚。重复使用4次后OPH@H-Au-TiO2保留了80%以上的初始降解活性。本研究为设计和构建多功能生物催化剂提供了新的见解,极大地拓展了酶催化的应用场景和工业价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Visible light assisted enzyme-photocatalytic cascade degradation of organophosphorus pesticides

The worldwide application of organophosphorus pesticides (OPs) has promoted agricultural development, but their gradual accumulation in soil and water can seriously affect the central nervous system of humans and other mammals. Organophosphorus hydrolase (OPH) is an effective enzyme that can catalyze the degradation of the residual OPs. However, the degradation products such as p-nitrophenol (p-NP) is still toxic. Thus, it is of great significance to develop a multi-functional support that can be simultaneously used for the immobilization of OPH and the further degradation of p-NP. Herein, a visible light assisted enzyme-photocatalytic integrated catalyst was constructed by immobilizing OPH on hollow structured Au-TiO2 (named OPH@H-Au-TiO2) for the degradation of OPs. The obtained OPH@H-Au-TiO2 can degrade methyl parathion to p-NP by OPH and then degrade p-NP to hydroquinone with low toxicity by using H-Au-TiO2 under visible light. OPH molecules were immobilized on H-Au-TiO2 through adsorption method to prepare OPH@H-Au-TiO2. After 2.5 h of reaction, methyl parathion is completely degraded, and about 82.64% of the generated p-NP is further degraded into hydroquinone. After reused for 4 times, the OPH@H-Au-TiO2 retains more than 80% of the initial degradation activity. This research presents a new insight in designing and constructing multi-functional biocatalyst, which greatly expands the application scenarios and industrial value of enzyme catalysis.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Green Chemical Engineering
Green Chemical Engineering Process Chemistry and Technology, Catalysis, Filtration and Separation
CiteScore
11.60
自引率
0.00%
发文量
58
审稿时长
51 days
期刊最新文献
OFC: Outside Front Cover Outside Back Cover Outside Back Cover OFC: Outside Front Cover Integration of physical information and reaction mechanism data for surrogate prediction model and multi-objective optimization of glycolic acid production
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1