Experimental Study on Targeting Brain Tumor with Ultrasound Nanoparticles Across Blood Brain Barrier

Glioma is a malignant tumor that originates in the brain and accounts for more than half of all brain tumors. The disease is associated with a high fatality rate. Satisfactory treatment cannot be achieved through conventional craniotomy. We use carboxymethyl chitosan-coated Fe3 O4 composite nanoparticles (CMCS-Fe3 O4 NPs) as a drug delivery platform, and installed transferrin (TRF) with a targeting function on the platform. The anticancer drug methotrexate (MTX) is loaded onto the composite nanomaterial to complete a new delivery system for nano-drug targeted treatment of glioma. The composite nanoparticle is not toxic to cells in vitro, but after loading with it is loaded with MTX, it significantly inhibits proliferation of C6 cells. In vivo experiments show that CMCS-Fe3 O4NPs-TRF-MTX has a marked therapeutic effect on glioma in mouse models. The nano complex quickly penetrates the blood-brain barrier and enters brain tissues. An aggregation effect is not obvious. MRI monitoring for up to 4.0 h shows that CMCS-Fe3 O4 NPs-TRF-MTX slowly releases drug molecules over an extended period, thus increasing the duration of drug activity.