植物生物辅助家鸡肠道细菌进化印记的分析

Q4 Biochemistry, Genetics and Molecular Biology Animal Gene Pub Date : 2023-08-01 DOI:10.1016/j.angen.2023.200153
Soundararajan Sowmiya , Ragothaman Prathiviraj , Joseph Selvin , R. Jasmine
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引用次数: 0

摘要

肠道微生物群是新陈代谢的重要组成部分,有助于分解进入消化道的复杂碳水化合物、蛋白质和脂质。由此产生的许多微生物代谢产物可能具有局部和全身影响,这可能对健康产生积极或消极的影响。微生物种群在肠道中的优势取决于器官中存在的化合物的数量。因此,我们专注于分析辣木作为植物生物补充剂在增强鸡模型中有益肠道微生物群方面的作用。益生菌通过多种机制改善鸡的肠道健康,包括紧密连接增强、营养吸收、生态位定植和与肠道病原体的共聚集。对从M.oleifera处理的鸡获得的肠道样本中的细菌进行了各种标准形态和生化测试、使用16S rRNA基因测序的基因型分类以及肠道细菌进化标记的评估,以确定它们是否符合益生菌特征的标准。有益肠道微生物对宿主的各种后果可能归因于细菌群落的过程和宿主影响相同过程的能力。根据我们的研究,宿主的系统发育分析和分类学在一系列宿主分类水平上显著影响了肠道菌群。然而,宿主生态学(即饮食)可以进一步改变这些肠道菌群,特别是在许多密切相关的宿主物种的情况下。
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Analysis of evolutionary imprints among the gut bacteria in phytobiotic supplemented Gallus gallus domesticus

The gut microbiota is an essential part of metabolism, assists in the breakdown of complex carbohydrates, proteins and lipids that enter into the digestive tract. Numerous microbial metabolites thus produced can have local and systemic effects which may influence health positively or negatively. The microbial population's dominance in the gut depends on the number of compounds present in the organ. Hence we have focused on analyzing the role of Moringa oleifera as phytobiotic supplementation in enhancing the beneficial gut microbiota in chicken models. Probiotics improve gut health in chickens through several mechanisms, including tight junction enhancement, nutrient uptake, niche colonization and co-aggregation with enteropathogens. The bacteria from the gut samples obtained from M.oleifera treated chickens were analyzed for various standard morphological and biochemical tests, genotypic classification using 16S rRNA gene sequencing and evaluation of evolutionary marks among the gut bacteria to determine whether they fit the criteria for probiotic traits. A variety of the consequences of beneficial gut microorganisms for hosts could attribute to bacterial communities' processes and the host's capacity for influencing the same processes. According to our study, phylogenetic analysis and taxonomy of the host significantly shape the intestinal flora across a range of host taxonomic levels. However, host ecology (i.e., diet) can further alter these gut flora, particularly in the case of many closely related host species.

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来源期刊
Animal Gene
Animal Gene Agricultural and Biological Sciences-Insect Science
自引率
0.00%
发文量
16
期刊介绍: Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.
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