印尼斑节对虾(Penaeus monodon)上对虾病毒的同时病原检测

Q4 Environmental Science Indonesian Aquaculture Journal Pub Date : 2023-06-30 DOI:10.15578/iaj.18.1.2023.79-86
I. Koesharyani, P. E. Sudaryatma, L. Gardenia, Y. Aryati, K. Mahardika, T. Mufidah
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引用次数: 0

摘要

多种病原体感染对印度尼西亚和世界各地的虾业造成了严重的经济影响,因为受感染虾的大量死亡和存活下来的多种异常。然而,多种病原体检测工具在对虾疾病中尚未得到广泛应用。本研究旨在开发和应用多重聚合酶链式反应(PCR)同时检测系统,检测由白斑综合征病毒(WSSV)、感染性皮下和造血坏死病毒(IHHNV)和单核杆状病毒(MBV)引起的自然感染。为了分析对虾的多种病原体感染,本研究设计并使用了三对针对对虾疾病DNA病毒的特异性引物。所有扩增均使用用于多重PCR测定的特定主混合物和从样品中标准化提取的核酸。这种mPCR检测通过对每种病毒进行多重PCR,在单管中成功扩增了三种病毒的DNA。基于这些结果,该研究证实,多病原体感染导致的群体死亡率最高,而不是WSSV、IHHNV或MBV的单一感染。这项研究还证实,mPCR检测是一种更快、更便宜、有效的方法,可以检测并随后防止多种病原体虾疾病的传播。
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SIMULTANEOUS PATHOGEN DETECTION OF SHRIMP VIRUSES ON CULTURED TIGER SHRIMPS (Penaeus monodon) IN INDONESIA
The multiple-pathogen infection causes severe economic impact to shrimp industry in Indonesia and worldwide due to mass mortality and multiple abnormalities of the survived infected shrimps. However, multiple-pathogen detection tools in shrimp diseases have not yet widely used. The purpose in this study was to develop and applied simultaneous detection system using multiplex polymerase chain reaction (PCR) assay from natural infections caused by white spot syndrome virus (WSSV), infectious hypodermal and haematopoietic necrosis virus (IHHNV) and monodon baculovirus (MBV) in Black tiger shrimp culture. To analyze multiple-pathogen infections in the shrimp, the study designed and used three pairs of specific primers targeting DNA virus from the shrimp diseases. All amplifications used a specific master mix for multiplex PCR assay and standardized extracted nucleic acid from the samples. This mPCR assay successfully amplified the DNA of three viruses in a single tube-run by multiplex PCR for each virus. Based on the results, the study confirms that multiple-pathogen infection contributes the highest mass mortality rather than from single infection by either WSSV, IHHNV or MBV. This study also confirms that the mPCR assay is a faster, cheaper, and efficient method to detect and subsequently prevent the spreading of multi-pathogen shrimp diseases.
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来源期刊
Indonesian Aquaculture Journal
Indonesian Aquaculture Journal Environmental Science-Water Science and Technology
CiteScore
0.50
自引率
0.00%
发文量
9
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