{"title":"LINC00665靶向miR-379-5p对肝癌细胞增殖、凋亡、迁移和侵袭的影响","authors":"Xiting Zhao, R. Ji","doi":"10.3760/CMA.J.ISSN.1007-8118.2020.03.008","DOIUrl":null,"url":null,"abstract":"Objective \nTo investigate the effect and mechanism of LINC00665 on hepatocellular carcinoma cell proliferation, migration, invasion and apoptosis. \n \n \nMethods \nFrom June 2013 to June 2018, 126 liver cancer tissue and adjacent tissue (more than 2 cm from the edge of liver cancer tissue) specimens were collected in the Third People's Hospital of Jinan, 86 male and 40 female were included, aged 25.0-72.0 (48.2±9.9) years. The expression level of LINC00665 in 126 liver cancer tissues and adjacent tissues were detected by qRT-PCR. The survival rate of hepatocellular carcinoma HCC9204 cells was determined by CCK8 assay. The apoptosis of HCC9204 cells was detected by flow cytometry, cell migration and invasion were detected by Transwell assay. And the dual-luciferase reporter assay system was implemented to investigate the correlations between LINC00665 and miR-379-5p. \n \n \nResults \nCompared with the adjacent tissues group, the expression level of LINC00665 in liver cancer tissues group was increased (1.00±0.10 vs. 1.82±0.18), with statistically significant difference (P 0.05); The level of miR-379-5p in the LINC00665 overexpression group (pcDNA3.1-LINC00665) was decreased [(1.01±0.10) vs (0.37±0.04)]; but was increased in the LINC00665 inhibition group (si-LINC00665)[(0.98±0.10) vs (1.66±0.17)], with statistically significant differences (P<0.05); Decreasing the content of LINC00665 and miR-379-5p, The cell survival rate was increased [(46.53±4.72)% vs. (82.26±8.34)%], the apoptosis rate was decreased (23.51±2.44)% vs. (12.07±1.21)%], and the number of migrating cells and invasive cells were increased [(54±6) vs. (92±9); (48±5) vs. (88±9)] when LINC00665 and miR-379-5p were inhibited the difference were statistically significant (all P<0.05). \n \n \nConclusions \nIn hepatocellular carcinoma HCC9204, LINC00665 targets the regulation of miR-379-5p expression, thereby regulating the proliferation, migration, invasion, and apoptosis of hepatocellular carcinoma HCC9204, which is a potential molecular target for liver cancer. \n \n \nKey words: \nLiver neoplasms; MiR-379-5p; Proliferation; Migration; Invasion; Apoptosis","PeriodicalId":10021,"journal":{"name":"中华肝胆外科杂志","volume":"26 1","pages":"187-191"},"PeriodicalIF":0.0000,"publicationDate":"2020-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of LINC00665 on hepatocellular carcinoma cell proliferation, apoptosis, migration and invasion by targeting miR-379-5p\",\"authors\":\"Xiting Zhao, R. Ji\",\"doi\":\"10.3760/CMA.J.ISSN.1007-8118.2020.03.008\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective \\nTo investigate the effect and mechanism of LINC00665 on hepatocellular carcinoma cell proliferation, migration, invasion and apoptosis. \\n \\n \\nMethods \\nFrom June 2013 to June 2018, 126 liver cancer tissue and adjacent tissue (more than 2 cm from the edge of liver cancer tissue) specimens were collected in the Third People's Hospital of Jinan, 86 male and 40 female were included, aged 25.0-72.0 (48.2±9.9) years. The expression level of LINC00665 in 126 liver cancer tissues and adjacent tissues were detected by qRT-PCR. The survival rate of hepatocellular carcinoma HCC9204 cells was determined by CCK8 assay. The apoptosis of HCC9204 cells was detected by flow cytometry, cell migration and invasion were detected by Transwell assay. And the dual-luciferase reporter assay system was implemented to investigate the correlations between LINC00665 and miR-379-5p. \\n \\n \\nResults \\nCompared with the adjacent tissues group, the expression level of LINC00665 in liver cancer tissues group was increased (1.00±0.10 vs. 1.82±0.18), with statistically significant difference (P 0.05); The level of miR-379-5p in the LINC00665 overexpression group (pcDNA3.1-LINC00665) was decreased [(1.01±0.10) vs (0.37±0.04)]; but was increased in the LINC00665 inhibition group (si-LINC00665)[(0.98±0.10) vs (1.66±0.17)], with statistically significant differences (P<0.05); Decreasing the content of LINC00665 and miR-379-5p, The cell survival rate was increased [(46.53±4.72)% vs. (82.26±8.34)%], the apoptosis rate was decreased (23.51±2.44)% vs. (12.07±1.21)%], and the number of migrating cells and invasive cells were increased [(54±6) vs. (92±9); (48±5) vs. (88±9)] when LINC00665 and miR-379-5p were inhibited the difference were statistically significant (all P<0.05). \\n \\n \\nConclusions \\nIn hepatocellular carcinoma HCC9204, LINC00665 targets the regulation of miR-379-5p expression, thereby regulating the proliferation, migration, invasion, and apoptosis of hepatocellular carcinoma HCC9204, which is a potential molecular target for liver cancer. \\n \\n \\nKey words: \\nLiver neoplasms; MiR-379-5p; Proliferation; Migration; Invasion; Apoptosis\",\"PeriodicalId\":10021,\"journal\":{\"name\":\"中华肝胆外科杂志\",\"volume\":\"26 1\",\"pages\":\"187-191\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-03-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华肝胆外科杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.1007-8118.2020.03.008\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华肝胆外科杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1007-8118.2020.03.008","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
Effect of LINC00665 on hepatocellular carcinoma cell proliferation, apoptosis, migration and invasion by targeting miR-379-5p
Objective
To investigate the effect and mechanism of LINC00665 on hepatocellular carcinoma cell proliferation, migration, invasion and apoptosis.
Methods
From June 2013 to June 2018, 126 liver cancer tissue and adjacent tissue (more than 2 cm from the edge of liver cancer tissue) specimens were collected in the Third People's Hospital of Jinan, 86 male and 40 female were included, aged 25.0-72.0 (48.2±9.9) years. The expression level of LINC00665 in 126 liver cancer tissues and adjacent tissues were detected by qRT-PCR. The survival rate of hepatocellular carcinoma HCC9204 cells was determined by CCK8 assay. The apoptosis of HCC9204 cells was detected by flow cytometry, cell migration and invasion were detected by Transwell assay. And the dual-luciferase reporter assay system was implemented to investigate the correlations between LINC00665 and miR-379-5p.
Results
Compared with the adjacent tissues group, the expression level of LINC00665 in liver cancer tissues group was increased (1.00±0.10 vs. 1.82±0.18), with statistically significant difference (P 0.05); The level of miR-379-5p in the LINC00665 overexpression group (pcDNA3.1-LINC00665) was decreased [(1.01±0.10) vs (0.37±0.04)]; but was increased in the LINC00665 inhibition group (si-LINC00665)[(0.98±0.10) vs (1.66±0.17)], with statistically significant differences (P<0.05); Decreasing the content of LINC00665 and miR-379-5p, The cell survival rate was increased [(46.53±4.72)% vs. (82.26±8.34)%], the apoptosis rate was decreased (23.51±2.44)% vs. (12.07±1.21)%], and the number of migrating cells and invasive cells were increased [(54±6) vs. (92±9); (48±5) vs. (88±9)] when LINC00665 and miR-379-5p were inhibited the difference were statistically significant (all P<0.05).
Conclusions
In hepatocellular carcinoma HCC9204, LINC00665 targets the regulation of miR-379-5p expression, thereby regulating the proliferation, migration, invasion, and apoptosis of hepatocellular carcinoma HCC9204, which is a potential molecular target for liver cancer.
Key words:
Liver neoplasms; MiR-379-5p; Proliferation; Migration; Invasion; Apoptosis
期刊介绍：
Chinese Journal of Hepatobiliary Surgery is an academic journal organized by the Chinese Medical Association and supervised by the China Association for Science and Technology, founded in 1995. The journal has the following columns: review, hot spotlight, academic thinking, thesis, experimental research, short thesis, case report, synthesis, etc. The journal has been recognized by Beida Journal (Chinese Journal of Humanities and Social Sciences).
Chinese Journal of Hepatobiliary Surgery has been included in famous databases such as Peking University Journal (Chinese Journal of Humanities and Social Sciences), CSCD Source Journals of China Science Citation Database (with Extended Version) and so on, and it is one of the national key academic journals under the supervision of China Association for Science and Technology.
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