{"title":"接受放疗和化疗的口腔癌症患者的口腔粘膜炎和微生物定植：三级牙科医院的前瞻性分析。","authors":"Nandhini Subramaniam, Arvind Muthukrishnan","doi":"10.1111/jicd.12454","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Aim</h3>\n \n <p>The ulcerative phase of oral mucositis following radiotherapy/chemotherapy for oral cancer colonizes bacteria, fungi and viruses. The role of a microbiota, specifically bacterial colonization in oral mucositis, is still unclear, and there is no existing data that correlates the shift in the bacterial colonization with mucositis severity. The aim of this study was to assess the bacterial colonization and study the <i>MCR-1</i> (mobilized colistin resistance), <i>VIM2</i> (β-lactam resistance), <i>TET(K) </i>(tetracycline resistance) and <i>bla</i><sub><i>KPC</i></sub> (carbapenem resistance) genes’ expression in isolated facultative anaerobes at 3 time points in oral mucositis patients undergoing radiotherapy and concomitant radiochemotherapy.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>A total of 24 oral cancer patients were divided into 2 groups: A (N = 12) undergoing radiotherapy; and B (N = 12) undergoing radiochemotherapy. Saliva was collected from all patients at 3 time intervals during the treatment. The isolated bacterial colonies were subjected to gene expression and analysis.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p><i>Staphylococcus aureus</i> (22%), <i>Staphylococcus epidermidis</i> (29%), <i>Pseudomonas aeruginosa</i> (28%), <i>Escherichia coli</i> (25%) and <i>Klebsiella pneumoniae</i> (26%) are the facultative anaerobes isolated from saliva. The bacterial isolates obtained during and at the end of therapy appeared to express a higher level of antibiotic-resistance genes (<i>VIM2</i>, <i>MCR-1</i>, <i>TET[K]</i>, <i>bla</i><sub><i>KPC</i></sub>) than those isolated at the onset of therapy.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>Bacterial colonization and gene expression varied during different stages of mucositis.</p>\n </section>\n </div>","PeriodicalId":16204,"journal":{"name":"Journal of Investigative and Clinical Dentistry","volume":"10 4","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/jicd.12454","citationCount":"76","resultStr":"{\"title\":\"Oral mucositis and microbial colonization in oral cancer patients undergoing radiotherapy and chemotherapy: A prospective analysis in a tertiary care dental hospital\",\"authors\":\"Nandhini Subramaniam, Arvind Muthukrishnan\",\"doi\":\"10.1111/jicd.12454\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Aim</h3>\\n \\n <p>The ulcerative phase of oral mucositis following radiotherapy/chemotherapy for oral cancer colonizes bacteria, fungi and viruses. The role of a microbiota, specifically bacterial colonization in oral mucositis, is still unclear, and there is no existing data that correlates the shift in the bacterial colonization with mucositis severity. The aim of this study was to assess the bacterial colonization and study the <i>MCR-1</i> (mobilized colistin resistance), <i>VIM2</i> (β-lactam resistance), <i>TET(K) </i>(tetracycline resistance) and <i>bla</i><sub><i>KPC</i></sub> (carbapenem resistance) genes’ expression in isolated facultative anaerobes at 3 time points in oral mucositis patients undergoing radiotherapy and concomitant radiochemotherapy.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>A total of 24 oral cancer patients were divided into 2 groups: A (N = 12) undergoing radiotherapy; and B (N = 12) undergoing radiochemotherapy. Saliva was collected from all patients at 3 time intervals during the treatment. The isolated bacterial colonies were subjected to gene expression and analysis.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p><i>Staphylococcus aureus</i> (22%), <i>Staphylococcus epidermidis</i> (29%), <i>Pseudomonas aeruginosa</i> (28%), <i>Escherichia coli</i> (25%) and <i>Klebsiella pneumoniae</i> (26%) are the facultative anaerobes isolated from saliva. The bacterial isolates obtained during and at the end of therapy appeared to express a higher level of antibiotic-resistance genes (<i>VIM2</i>, <i>MCR-1</i>, <i>TET[K]</i>, <i>bla</i><sub><i>KPC</i></sub>) than those isolated at the onset of therapy.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>Bacterial colonization and gene expression varied during different stages of mucositis.</p>\\n </section>\\n </div>\",\"PeriodicalId\":16204,\"journal\":{\"name\":\"Journal of Investigative and Clinical Dentistry\",\"volume\":\"10 4\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-08-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1111/jicd.12454\",\"citationCount\":\"76\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Investigative and Clinical Dentistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jicd.12454\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Investigative and Clinical Dentistry","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jicd.12454","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
Oral mucositis and microbial colonization in oral cancer patients undergoing radiotherapy and chemotherapy: A prospective analysis in a tertiary care dental hospital
Aim
The ulcerative phase of oral mucositis following radiotherapy/chemotherapy for oral cancer colonizes bacteria, fungi and viruses. The role of a microbiota, specifically bacterial colonization in oral mucositis, is still unclear, and there is no existing data that correlates the shift in the bacterial colonization with mucositis severity. The aim of this study was to assess the bacterial colonization and study the MCR-1 (mobilized colistin resistance), VIM2 (β-lactam resistance), TET(K) (tetracycline resistance) and blaKPC (carbapenem resistance) genes’ expression in isolated facultative anaerobes at 3 time points in oral mucositis patients undergoing radiotherapy and concomitant radiochemotherapy.
Methods
A total of 24 oral cancer patients were divided into 2 groups: A (N = 12) undergoing radiotherapy; and B (N = 12) undergoing radiochemotherapy. Saliva was collected from all patients at 3 time intervals during the treatment. The isolated bacterial colonies were subjected to gene expression and analysis.
Results
Staphylococcus aureus (22%), Staphylococcus epidermidis (29%), Pseudomonas aeruginosa (28%), Escherichia coli (25%) and Klebsiella pneumoniae (26%) are the facultative anaerobes isolated from saliva. The bacterial isolates obtained during and at the end of therapy appeared to express a higher level of antibiotic-resistance genes (VIM2, MCR-1, TET[K], blaKPC) than those isolated at the onset of therapy.
Conclusion
Bacterial colonization and gene expression varied during different stages of mucositis.
期刊介绍：
Journal of Investigative and Clinical Dentistry (JICD) aims to publish quality, peer reviewed original research and topical reviews on all aspects of investigative and clinical dentistry and craniofacial research, including molecular studies related to oral health and disease. Although international in outlook, the Editor especially encourages papers from the Asia Pacific. The journal also aims to provide clinicians, scientists and students of dentistry with a knowledge transfer platform for rapid publication of reports through an international journal, which will be available free online until 2012. Its scope, therefore, is broad, inclusive and international, but with a particular focus on Asia Pacific. The Editor welcomes manuscripts in the following key thematic areas in oral and maxillofacial sciences: Community Dentistry and Oral Epidemiology, Conservative Dentistry, Dental Biomaterials, Dental Pedagogy, Endodontics and Traumatology, Implant Dentistry, Oral Biosciences, Oral and Maxillofacial Surgery, Oral Medicine, Oral Pathology and Oral Microbiology, Orthodontics, Oral Radiology, Oral Rehabilitation, Paedodontics, Periodontology and Periodontal Medicine.
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