越南社会主义共和国艾滋病毒感染者中乙型和丁型肝炎病毒的患病率

Y. Ostankova, A. Semenov, E. B. Zueva, E. Serikova, A. Shchemelev, D. Valutite, H. K. T. Huynh, A. Totolian
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引用次数: 0

摘要

本研究的目的是估计南越HIV感染者中乙型肝炎和丁型肝炎病毒的流行率。材料和方法。研究材料以从接受抗逆转录病毒治疗的越南社会主义共和国感染艾滋病毒的居民身上采集的316份血清样本为代表。通过对HBsAg、HBs IgG、HBcore IgG、抗HDV、DNA HBV和RNA HDV的定性检测,检查受试者是否存在HBV标志物。获得了23例HIV+HBV+HDV共感染患者的HBV和HDV全基因组核苷酸序列。HBV和HDV的扩增和随后的测序是使用嵌套PCR进行的,这对重叠引物分别位于完整的HBV和HD病毒基因组的两侧。后果HBV和HDV的血清学标志物的检出率分别为:HBsAg-9.17%、抗-HBs-Ig G-10.44%、抗-HBcre-Ig G-42.08%、总抗-HDV 9.81%。HBsAg阳性者和HBsAg阴性者HDV RNA检出率分别为24.13%和21.62%,分别占HBV阳性者和总组的22.33%和7.27%。在系统发育分析中,HBV亚型B4(60.89%)在HIV感染患者中占主导地位,而C1(21.73%)、B2(8.7%)、C2(4.34%)和C5(4.34%,以及血清阴性HDV在OBI患者中的流行率表明,需要在肝炎高发区使用PCR对普通人群,特别是高危人群进行乙型肝炎和丁型肝炎筛查。
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Prevalence of hepatitis B and D viruses in HIV-infected persons in the Socialist Republic of Vietnam
The aim of this study was to estimate the prevalence of hepatitis B and D viruses among HIV-infected residents of South Vietnam.Materials and methods. The study material was represented by 316 blood serum samples collected from HIV-infected residents of the Socialist Republic of Vietnam taking antiretroviral therapy. The subjects were examined for the presence of HBV markers with a qualitative detection of HBsAg, HBs IgG, HBcore IgG, anti-HDV, DNA HBV, and RNA HDV. HBV and HDV complete genomes nucleotide sequences were obtained for 23 samples from HIV+HBV+HDV co-infected patients. Amplification and subsequent sequencing of HBV and HDV were performed using nested PCR with pair’s overlapping primers jointly flanking the complete HBV and HDV genomes, respectively.Results. Serological markers of HBV and HDV were presented in the following ratios: HBsAg — 9.17%, anti-HBs Ig G — 10.44%, anti-HBcore Ig G — 42.08%, total anti-HDV — 9.81%. HBV DNA was detected in 32.58% of cases, including 23.41% of HBsAg-negative individuals. HDV RNA was detected in 24.13% of HBsAg-positive individuals and 21.62% of HBsAg-negative, which amounted to 22.33% of HBV-positive individuals and 7.27% of the total group, respectively. In phylogenetic analysis, HBV subgenotype B4 (60.89%) prevailed among HIV-infected patients compared to C1 (21.73%), B2 (8.7%), C2 (4.34%) and C5 (4.34%). Phylogenetic analysis of HDV nucleotide sequences showed the prevalence of HDV genotype 1 (78.26%) compared to genotype 2 (21.74%). The hepatitis Delta virus prevalence in patients with HIV+HBV coinfection, and the prevalence of seronegative HDV in patients with OBI indicate the need to use PCR in hepatitis highly endemic regions for hepatitis B and hepatitis D screening of the general population and especially those at-risk groups. 
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来源期刊
HIV Infection and Immunosuppressive Disorders
HIV Infection and Immunosuppressive Disorders Medicine-Infectious Diseases
CiteScore
0.70
自引率
0.00%
发文量
37
期刊介绍: In the scientific-practical journal "HIV Infection and Immunosuppressive Disorders", published various issues of HIV medicine (epidemiology, molecular mechanisms of pathogenesis to the development of educational programs) leading scientists of Russia and countries of CIS, USA, as well as practical healthcare professionals working in research centers, research institutes, universities, clinics where done basic medical work. A special place on the pages of the publication is given to basic and clinical research, analytical reviews of contemporary and foreign reports, the provision of medical care for various diseases.
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