Application of innovative technology of biolayer interferometry in basic research in the development of new vaccines

One of the effective methods for real-time protein analysis is based on the phenomenon of light interference and is called "biolayer interferometry". The patented Bio-Layer Interferometry (BLI) technology, which underlies ForteBio Octet platform, provides surface interaction analysis for disposable fiber optic biosensors. The article presents the technology of interferometry of the biolayer of BLI molecules, which can be used to determine the kinetics and affinity, for the specific quantitative determination of molecules bound to the biosensor. The purpose of the review was to summarize scientific data on the use of the interferometry technology of the biolayer of BLI molecules in determining the concentration of proteins and studying the kinetics of interaction using Octet systems when creating new vaccines in many ways. BLI biolayer interferometry technology allows protein concentration measurements to be performed without using of labels or auxiliary reagents, even in untreated media. It is characterized by a sensitivity up to several ng/cm3, provides accurate quantitative changes in a few seconds, and not in many hours like traditional methods (ELISA, HPL Coder). The affinity, concentration, and binding kinetics of the studied proteins can be measured in 4- ml drop samples directly on the laboratory table. The technology of interferometry of the biolayer of BLI molecules can be used by researchers to select the most immunogenic epitopes in the molecular structure of a pathogen, it is also possible to conduct studies on the characterization and recognition, diversity and distribution of pathogens, affinity for antibodies, it is possible to characterize the immune response of the host, to study molecular interactions between the pathogen and the host, and to carry out therapeutic and clinical research. Currently, the technology of interferometry of the biolayer of BLI molecules has already been used in fundamental studies of pathogens that cause HIV, herpes, Ebola, influenza A H7N9, Dengue, malaria, Zika virus, diphtheria, tuberculosis, listeriosis, gastroenteritis, respiratory pathology, including the COVID outbreak. -19.